scholarly journals In vitro genotoxicity of dibutyl phthalate on A549 lung cells at air‐liquid interface in exposure concentrations relevant at workplaces

2021 ◽  
Author(s):  
Stephanie Binder ◽  
Xin Cao ◽  
Stefanie Bauer ◽  
Narges Rastak ◽  
Evelyn Kuhn ◽  
...  
Keyword(s):  
Dibutyl Phthalate ◽  
Liquid Interface ◽  
Lung Cells ◽  
Air Liquid Interface ◽  
A549 Lung ◽  
In Vitro Genotoxicity

scholarly journals In vitro genotoxicity of airborne Ni-NP in air-liquid interface

2017 ◽  
Vol 37 (12) ◽  
pp. 1420-1427 ◽  
Author(s):  
Siiri Latvala ◽  
Daniel Vare ◽  
Hanna L. Karlsson ◽  
Karine Elihn
Keyword(s):  
Liquid Interface ◽  
Air Liquid Interface ◽  
In Vitro Genotoxicity

scholarly journals Exposure of silver-nanoparticles and silver-ions to lung cells in vitro at the air-liquid interface

2013 ◽  
Vol 10 (1) ◽  
pp. 11 ◽  
Author(s):  
Fabian Herzog ◽  
Martin JD Clift ◽  
Flavio Piccapietra ◽  
Renata Behra ◽  
Otmar Schmid ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Silver Ions ◽  
Liquid Interface ◽  
Lung Cells ◽  
Air Liquid Interface

Zwischen Luft und Wasser – Air-Liquid-Interface-Kulturen als In-vitro-Modell des Respirationstrakts

2020 ◽  
Author(s):  
S. Runft ◽  
L. Burigk ◽  
A. Lehmbecker ◽  
K. Schöne ◽  
D. Waschke ◽  
...  
Keyword(s):  
Liquid Interface ◽  
Air Liquid Interface

scholarly journals In vitro toxicity evaluation of single walled carbon nanotubes on human A549 lung cells

2007 ◽  
Vol 21 (3) ◽  
pp. 438-448 ◽  
Author(s):  
Maria Davoren ◽  
Eva Herzog ◽  
Alan Casey ◽  
Benjamin Cottineau ◽  
Gordon Chambers ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Single Walled Carbon Nanotubes ◽  
In Vitro Toxicity ◽  
Lung Cells ◽  
Toxicity Evaluation ◽  
Single Walled Carbon ◽  
Walled Carbon Nanotubes ◽  
Human A549 ◽  
A549 Lung

scholarly journals Genotoxic and inflammatory effects of spruce and brown coal briquettes combustion aerosols on lung cells at the air-liquid interface

2021 ◽  
pp. 150489
Author(s):  
Tuukka Ihantola ◽  
Maija-Riitta Hirvonen ◽  
Mika Ihalainen ◽  
Henri Hakkarainen ◽  
Olli Sippula ◽  
...  
Keyword(s):  
Brown Coal ◽  
Liquid Interface ◽  
Lung Cells ◽  
Combustion Aerosols ◽  
Air Liquid Interface

scholarly journals Real-time measurement of cellular bioenergetics in fully differentiated human nasal epithelial cells grown at air-liquid-interface

2020 ◽  
Vol 318 (6) ◽  
pp. L1158-L1164
Author(s):  
Emily Mavin ◽  
Bernard Verdon ◽  
Sean Carrie ◽  
Vinciane Saint-Criq ◽  
Jason Powell ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Real Time ◽  
Aerobic Glycolysis ◽  
Airway Epithelial Cells ◽  
Respiratory Epithelium ◽  
Liquid Interface ◽  
Human Nasal Epithelial Cells ◽  
Respiratory Epithelial ◽  
Air Liquid Interface

Shifts in cellular metabolic phenotypes have the potential to cause disease-driving processes in respiratory disease. The respiratory epithelium is particularly susceptible to metabolic shifts in disease, but our understanding of these processes is limited by the incompatibility of the technology required to measure metabolism in real-time with the cell culture platforms used to generate differentiated respiratory epithelial cell types. Thus, to date, our understanding of respiratory epithelial metabolism has been restricted to that of basal epithelial cells in submerged culture, or via indirect end point metabolomics readouts in lung tissue. Here we present a novel methodology using the widely available Seahorse Analyzer platform to monitor real-time changes in the cellular metabolism of fully differentiated primary human airway epithelial cells grown at air-liquid interface (ALI). We show increased glycolytic, but not mitochondrial, ATP production rates in response to physiologically relevant increases in glucose availability. We also show that pharmacological inhibition of lactate dehydrogenase is able to reduce glucose-induced shifts toward aerobic glycolysis. This method is timely given the recent advances in our understanding of new respiratory epithelial subtypes that can only be observed in vitro through culture at ALI and will open new avenues to measure real-time metabolic changes in healthy and diseased respiratory epithelium, and in turn the potential for the development of novel therapeutics targeting metabolic-driven disease phenotypes.

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