scholarly journals 3Rs friendly study designs facilitate rat liver and blood micronucleus assays andPig‐agene mutation assessments: Proof‐of‐concept with 13 reference chemicals

2019 ◽  
Vol 60 (8) ◽  
pp. 704-739 ◽  
Author(s):  
Stephen D. Dertinger ◽  
Svetlana L. Avlasevich ◽  
Dorothea K. Torous ◽  
Priyanka Singh ◽  
Sumee Khanal ◽  
...  
Keyword(s):  
Rat Liver ◽  
Proof Of Concept ◽  
Study Designs ◽  
Rat Liver And Blood

Proof of concept studies for tissue-protective agents in multiple sclerosis

2009 ◽  
Vol 15 (5) ◽  
pp. 542-546 ◽  
Author(s):  
LR Mehta ◽  
SR Schwid ◽  
DL Arnold ◽  
GR Cutter ◽  
S Aradhye ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Final Visit ◽  
Proof Of Concept ◽  
Eligibility Criteria ◽  
Double Blind ◽  
Protective Agents ◽  
Parallel Group ◽  
Acute Exacerbations ◽  
Study Designs ◽  
Size Estimates

Background There is considerable interest in tissue-protective treatments for multiple sclerosis (MS). Methods and Objectives We convened a group of MS clinical trialists and related researchers to discuss designs for proof of concept studies utilizing currently available data and assessment methods. Results Our favored design was a randomized, double-blind, parallel-group study of active treatment versus placebo focusing on changes in brain volume from a post-baseline scan (3–6 months after starting treatment) to the final visit 1 year later. Study designs aimed at reducing residual deficits following acute exacerbations are less straightforward, depending greatly on the anticipated rapidity of treatment effect onset. Conclusions The next step would be to perform one or more studies of potential tissue-protective agents with these designs in mind, creating the longitudinal data necessary to refine endpoint selection, eligibility criteria, and sample size estimates for future trials.

N-Acetyltransferases of Rat Liver and Blood: Substrate Specificities

Enzyme ◽  
1980 ◽  
Vol 25 (5) ◽  
pp. 309-315 ◽  
Author(s):  
R. Gollamudi ◽  
B. Muniraju ◽  
E.C. Schreiber
Keyword(s):  
Rat Liver ◽  
Substrate Specificities ◽  
Rat Liver And Blood

Concentrations of Acetoacetate and d-(−)-3-Hydroxybutyrate in Rat Liver and Blood

1965 ◽  
Vol 94 (2) ◽  
pp. 17C-19C ◽  
Author(s):  
M. N. Berry ◽  
D. H. Williamson ◽  
M. B. Wilson
Keyword(s):  
Rat Liver ◽  
Rat Liver And Blood

Effect of Bioregulators Isolated from Rat Liver and Blood Serum on the State of Murine Liver in Roller Organotypic Culture after CCl4-Induced Fibrosis

2016 ◽  
Vol 161 (4) ◽  
pp. 604-609 ◽  
Author(s):  
D. S. Nalobin ◽  
M. S. Krasnov ◽  
S. I. Alipkina ◽  
M. S. Syrchina ◽  
V. P. Yamskova ◽  
...  
Keyword(s):  
Blood Serum ◽  
Rat Liver ◽  
Organotypic Culture ◽  
The State ◽  
Murine Liver ◽  
Rat Liver And Blood

Initial Polymerization Sites Indicated During Mitochondrial Oxidation of Diaminobenzidine after Toluene Treatment

1977 ◽  
Vol 35 ◽  
pp. 408-409
Author(s):  
W. A. Shannon ◽  
M. A. Matlib
Keyword(s):  
Membrane Permeability ◽  
Cytochrome Oxidase ◽  
Rat Liver ◽  
Precise Definition ◽  
Cytochemical Localization ◽  
Oxidative Reaction ◽  
Mitochondrial Oxidation ◽  
Definition Of ◽  
Exogenous Substrates

Numerous studies have dealt with the cytochemical localization of cytochrome oxidase via cytochrome c. More recent studies have dealt with indicating initial foci of this reaction by altering incubation pH (1) or postosmication procedure (2,3). The following study is an attempt to locate such foci by altering membrane permeability. It is thought that such alterations within the limits of maintaining morphological integrity of the membranes will ease the entry of exogenous substrates resulting in a much quicker oxidation and subsequently a more precise definition of the oxidative reaction.The diaminobenzidine (DAB) method of Seligman et al. (4) was used. Minced pieces of rat liver were incubated for 1 hr following toluene treatment (5,6). Experimental variations consisted of incubating fixed or unfixed tissues treated with toluene and unfixed tissues treated with toluene and subsequently fixed.

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