Folate deficiency in human peripheral blood lymphocytes induces chromosome 8 aneuploidy but this effect is not modified by riboflavin

2009 ◽  
pp. NA-NA ◽  
Author(s):  
Juan Ni ◽  
Lin Lu ◽  
Michael Fenech ◽  
Xu Wang
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Folate Deficiency ◽  
Peripheral Blood Lymphocytes ◽  
Chromosome 8 ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes

scholarly journals In vitrofolate deficiency induces apoptosis by a p53, Fas (Apo-1, CD95) independent, bcl-2 related mechanism in phytohaemagglutinin-stimulated human peripheral blood lymphocytes

2006 ◽  
Vol 95 (5) ◽  
pp. 870-878 ◽  
Author(s):  
Hui-Li Lin ◽  
Chung-Jern Chen ◽  
Wen-Chan Tsai ◽  
Jeng-Hsien Yen ◽  
Hong-Wen Liu
Keyword(s):  
Folic Acid ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Folate Deficiency ◽  
Peripheral Blood Lymphocytes ◽  
S Phase ◽  
Fas Antigen ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Phase Accumulation

Invitrofolate deficiency is associated with S phase accumulation and apoptosis in various cell types. To investigate the role of p53 and two apoptosis-related molecules, bcl-2 and Fas antigen (Apo-1, CD95), in the mechanism whereby folate-deficient lymphocytes accumulate and undergo apoptosis in the S phase, normal human peripheral blood lymphocytes were cultured for 3–9 d in control medium or in specially ordered and formulated HAM’ F-10 medium lacking folic acid, thymidine and hypoxanthine. Cells were stimulated with phytohaemagglutinin for the final 72 h prior to harvesting. The results indicate that p53 expression was downregulated in folate-deficient lymphocytes when compared with the control lymphocytes during the relevant period of S phase accumulation and apoptosis. In addition, folate deficiency was also found to downregulate IL-2, Fas antigen and bcl-2 expression, in terms of either mRNA or protein levels. The downregulation of Fas antigen suggests that folate deficiency-induced apoptosis probably does not occur via the Fas pathway. As IL-2 is a known inducer of bcl-2, and the downregulation of bcl-2 induces apoptosis, the downregulation of IL-2 and bcl-2 is suggested to play an important role in apoptosis. The complete rescue of folate-deficient lymphocytes from apoptosis was achieved by folic acid, thymidine or hypoxanthine alone or thymidine and hypoxanthine in combination. These results suggest that IL-2 depletion by folate deficiency in lymphocytes reduces the bcl-2 level, thereby triggering deoxynucleoside triphosphate pool imbalance and p53-independent apoptosis.

scholarly journals Analysis of P-glycoprotein-mediated membrane transport in human peripheral blood lymphocytes using the UIC2 shift assay

Cytometry ◽  
2003 ◽  
Vol 53A (2) ◽  
pp. 67-78 ◽  
Author(s):  
Suk W. Park ◽  
Noureddine Lomri ◽  
Luiz A. Simeoni ◽  
John P. Fruehauf ◽  
Eugene Mechetner
Keyword(s):  
Membrane Transport ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
P Glycoprotein
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