scholarly journals Acetate formation during recombinant protein production in Escherichia coli K‐12 with an elevated NAD(H) pool

2019 ◽  
Vol 19 (11) ◽  
pp. 770-780 ◽  
Author(s):  
Qi Han ◽  
Mark A. Eiteman
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Acetate Formation ◽  
K 12

scholarly journals Expression of an Anaplerotic Enzyme, Pyruvate Carboxylase, Improves Recombinant Protein Production in Escherichia coli

2002 ◽  
Vol 68 (11) ◽  
pp. 5620-5624 ◽  
Author(s):  
J. C. March ◽  
M. A. Eiteman ◽  
E. Altman
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein ◽  
Pyruvate Carboxylase ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Tricarboxylic Acid ◽  
Acetate Concentration ◽  
Glucose Carbon ◽  
Model Protein ◽  
Acetate Formation

ABSTRACT Anaplerotic enzyme reactions are those which replenish tricarboxylic acid intermediates that are withdrawn for the synthesis of biomass. In this study, we examined recombinant protein production in Escherichia coli containing activity in an additional anaplerotic enzyme, pyruvate carboxylase. In batch fermentations, the presence of pyruvate carboxylase resulted in 68% greater production of the model protein, β-galactosidase, 41% greater cell yield, and 57% lower acetate concentration. We discuss why these results indicate that acetate concentration does not limit cell growth and protein synthesis, as predicted by other researchers, and suggest instead that the rate of acetate formation represents an inefficient consumption of glucose carbon, which is reduced by the presence of pyruvate carboxylase.

scholarly journals Isolating Escherichia coli strains for recombinant protein production

2016 ◽  
Vol 74 (5) ◽  
pp. 891-908 ◽  
Author(s):  
Susan Schlegel ◽  
Pierre Genevaux ◽  
Jan-Willem de Gier
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Protein Production

scholarly journals Decoupling of recombinant protein production from Escherichia coli cell growth enhances functional expression of plant Leloir glycosyltransferases

2019 ◽  
Vol 116 (6) ◽  
pp. 1259-1268 ◽  
Author(s):  
Martin Lemmerer ◽  
Juergen Mairhofer ◽  
Alexander Lepak ◽  
Karin Longus ◽  
Rainer Hahn ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Growth ◽  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Functional Expression ◽  
Coli Cell

scholarly journals Recombinant protein production in an Escherichia coli reduced genome strain

2007 ◽  
Vol 9 (2) ◽  
pp. 133-141 ◽  
Author(s):  
Shamik S. Sharma ◽  
Frederick R. Blattner ◽  
Sarah W. Harcum
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Reduced Genome
Sign in / Sign up

Export Citation Format

Share Document