scholarly journals Low cell surface hydrophobicity is one of the key factors for high butanol tolerance of Lactic acid bacteria

2018 ◽  
Vol 19 (2) ◽  
pp. 133-142 ◽  
Author(s):  
Penka Petrova ◽  
Flora Tsvetanova ◽  
Kaloyan Petrov
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Cell Surface ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Key Factors ◽  
Butanol Tolerance

scholarly journals Development of Selenized Lactic Acid Bacteria and their Selenium Bioaccummulation Capacity

Fermentation ◽  
2020 ◽  
Vol 6 (3) ◽  
pp. 91
Author(s):  
Gabriela Krausova ◽  
Antonin Kana ◽  
Ivana Hyrslova ◽  
Iva Mrvikova ◽  
Miloslava Kavkova
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Cell Surface ◽  
Sodium Selenite ◽  
Antioxidant Properties ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
In Vivo Studies ◽  
Dpph Method

Selenized lactic acid bacteria (LAB) represent potentially safe and effective sources of selenium (Se), essential for human health, as lactic acid fermentation improves Se bioavailability and reduces its toxicity. LAB are generally recognized as safe (GRAS) and widely used in fermented dairy products. To facilitate selenized LAB implementation as a functional food, we developed and characterized new Se-enriched strains based on the food industry commercial strains Streptococcus thermophilus CCDM 144 and Enterococcus faecium CCDM 922A as representatives of two LAB genera. We evaluated Se bioaccumulation capacity, Se biotransformation and growth ability in the presence of different sodium selenite concentrations (0–50 mg/L), and antioxidant properties (2, 2-diphenyl-1-picrylhydrazyl (DPPH) method) and cell surface hydrophobicity between Se-enriched and parental strains in vitro. Sodium selenite addition did not negatively influence growth of either strain; thus, 50 mg/L was chosen as the optimal concentration based on strain accumulation capacity. Selenization improved the antioxidant properties of both strains and significantly increased their cell surface hydrophobicity (p < 0.05). To our knowledge, this represents the first report of Se-enriched strain hydrophobicity as well as the first on Se speciation in families Enterococcaceae and Streptococcaceae. Moreover, both tested strains demonstrated good potential for Se-enrichment, providing a foundation for further in vitro and in vivo studies to confirm the suitability of these Se-enriched strains for industrial applications.

scholarly journals Probiotic and Antioxidant Properties of Lactic Acid Bacteria Isolated from Indigenous Fermented Tea Leaves (Miang) of North Thailand and Promising Application in Synbiotic Formulation

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 195
Author(s):  
Kridsada Unban ◽  
Wirunya Chaichana ◽  
Sasitorn Baipong ◽  
Aliyu Dantani Abdullahi ◽  
Apinun Kanpiengjai ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Cell Surface ◽  
Antioxidant Properties ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Rrna Gene ◽  
High Tolerance ◽  
Effective Response ◽  
Gastrointestinal Conditions

Miang, a traditional fermented tea from Northern Thailand, potentially hosts beneficial probiotic bacteria. A total of 133 isolates of lactic acid bacteria (LAB) isolated from Miang were evaluated for probiotic potential. Among them, 5 strains showed high tolerance to bile and acidic conditions and were selected for further evaluation. All selected strains showed inhibitory activity against human pathogens, including Bacillus cereus, Staphylococcus aureus, and Salmonella ser. Typhimurium. Nucleotide sequences analysis of the 16S rRNA gene revealed that 3 isolates were identified as Lactobacillus pentosus; the remaining were L. plantarum and Pediococcus pentosaceus, respectively. All 5 strains showed a high survival rate of more than 90% when exposed to simulated gastrointestinal conditions and were also susceptible to antibiotics such as erythromycin, tetracycline, and gentamycin, and resistant to vancomycin, streptomycin, and polymycin. In addition, the selected isolates exhibited different degrees of cell surface hydrophobicity (58.3–92.9%) and auto-aggregation (38.9–46.0%). The antioxidant activity reflected in DPPH scavenging activities of viable cells and their cell-free culture supernatants (CFCS) were also found in selected LAB isolates. Moreover, selected LAB isolates showed ability to grow on commercial prebiotics (GOS, FOS or XOS). The preliminary study of spray-drying using cyclodextrin as thermoprotectant suggested that all strains can be designed as a powdered formulation. L. pentosus A14-6 was the best strain, with high tolerance against simulated gastrointestinal conditions, high cell surface hydrophobicity, effective response to tested commercial oligosaccharides, especially XOS, and the highest cell antioxidant properties. L. pentosus A14-6 was therefore targeted for further applications in food and synbiotic applications.

scholarly journals Isolation and probiotic potential of lactic acid bacteria from swine feces for feed additive composition

2021 ◽  
Vol 204 (1) ◽  
Author(s):  
Katarzyna Marchwińska ◽  
Daniela Gwiazdowska
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bacterial Resistance ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Feed Additive ◽  
Rrna Gene ◽  
Probiotic Properties ◽  
Probiotic Potential ◽  
High Viability

AbstractAnimal microbiota is becoming an object of interest as a source of beneficial bacteria for commercial use. Moreover, the escalating problem of bacterial resistance to antibiotics is threatening animals and humans; therefore, in the last decade intensive search for alternative antimicrobials has been observed. In this study, lactic acid bacteria (LAB) were isolated from suckling and weaned pigs feces (376) and characterized to determine their functional properties and usability as pigs additives. Selection of the most promising LAB was made after each stage of research. Isolates were tested for their antimicrobial activity (376) and susceptibility to antibiotics (71). Selected LAB isolates (41) were tested for the production of organic acids, enzymatic activity, cell surface hydrophobicity and survival in gastrointestinal tract. Isolates selected for feed additive (5) were identified by MALDI-TOF mass spectrometry and partial sequence analysis of 16S rRNA gene, represented by Lentilactobacillus, Lacticaseibacillus (both previously classified as Lactobacillus) and Pediococcus genus. Feed additive prototype demonstrated high viability after lyophilization and during storage at 4 °C and − 20 °C for 30 days. Finally, feed additive was tested for survival in simulated alimentary tract of pigs, showing viability at the sufficient level to colonize the host. Studies are focused on obtaining beneficial strains of LAB with probiotic properties for pigs feed additive.

scholarly journals Screening for Probiotic Potential of Lactobacillus Rhamnosus Strain CRD4

2020 ◽  
Vol 11 (3) ◽  
pp. 10174-10184
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Rhamnosus ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Zone Of Inhibition ◽  
Probiotic Potential ◽  
16S Rna ◽  
Acid And Bile Tolerance ◽  
Gene Bank Accession

Lactic acid bacteria are the foremost used probiotic worldwide. Its successful application in manufacturing dairy products and probiotic foods makes it a promising industrial prerequisite. The objective of the present investigation was to isolate, identify, and molecularly characterize Lactic acid bacteria from local dairy samples of Odisha state of India and explores its probiotic traits. One potential strain was isolated using a selective Lactobacillus-MRS agar medium. The biochemical studies illustrated the bacteria were gram-positive, catalyze (-ve), and non-motile. The taxonomical diversity of the bacterium was analyzed by 16s RNA sequencing and classified as Lactobacillus rhamnosus strain CRD4 with gene bank accession no [MG573074]. Further, the selected strain was screened for its probiotic competence of lower acid and bile tolerance. The result confirmed that lactobacillus strain successfully defended the low pH and bile stress and acclaimed 70% cell surface hydrophobicity. Antibiotic studies obtained confirmed the possible resistance of the strain. The maximum zone of inhibition was expressed in diameter 42mm against Ciprofloxacin. In conclusion, based upon the above results, Lactobacillus rhamnosus can be a profound probiotic candidate.

scholarly journals How Capsular Exopolysaccharides Affect Cell Surface Properties of Lactic Acid Bacteria

2020 ◽  
Vol 8 (12) ◽  
pp. 1904
Author(s):  
Carsten Nachtigall ◽  
Cordula Vogel ◽  
Harald Rohm ◽  
Doris Jaros
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Cell Surface ◽  
Surface Properties ◽  
Cell Damage ◽  
Cell Surface Hydrophobicity ◽  
Textural Properties ◽  
Fermented Foods ◽  
Cell Surface Properties ◽  
The Impact

Some lactic acid bacteria are able to produce exopolysaccharides that, based on localization, can be distinguished in free and capsular or cell-bound exopolysaccharides (CPS). Up to now, the former were the focus of current research, mainly because of the technofunctional benefits they exhibit on fermented dairy products. On the other hand, CPS affect the surface properties of bacteria cells and thus also the textural properties of fermented foods, but data are very scarce. As the cell surface properties are strongly strain dependent, we present a new approach to investigate the impact of CPS on cell surface hydrophobicity and moisture load. CPS positive and negative Streptococcus thermophilus and Weissella cibaria were subjected to ultrasonication suitable to detach CPS without cell damage. The success of the method was verified by scanning electron and light microscopy as well as by cultivation experiments. Before applying ultrasonication cells with CPS exhibiting an increased hydrophilic character, enhanced moisture load, and faster water adsorption compared to the cells after CPS removal, emphasizing the importance of CPS on the textural properties of fermented products. The ultrasonic treatment did not alter the cell surface properties of the CPS negative strains.

scholarly journals Molecular identification of native lactic acid bacteria isolated from curd samples with probiotic potential

2019 ◽  
Vol 9 (6) ◽  
pp. 4591-4597
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Casei ◽  
Biochemical Study ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Fermented Foods ◽  
Bacterial Strains ◽  
Probiotic Potential ◽  
Acid And Bile Tolerance

Fermented foods are high in nutrient content than any other category of foods due to the presence of live microorganisms called probiotics. Its application in manufacturing of dairy foods and role in different types of disease prevention ranks it as the most exuberant. The aim of the present study was to isolate and identify lactic acid bacteria from native curd samples collected from dairy farms of Odisha state of India and explore its probiotic potential. Three morphologically distinct bacterial strains were isolated using MRS agar plates. The biochemical study confirmed that all the isolates were gram-positive. The molecular approaches were used to analyze the taxonomical diversity of isolates. 16 S rRNA sequencing was carried out and the bacterial isolates were taxonomically classified as Lactobacillus sp., Lactobacillus plantarum, Lactobacillus casei with NCBI Gene bank accession number [MG573071], [MG573072] and [MG573073] respectively. Further, the isolated bacterial strains were screened for their acid and bile tolerance competence as a principal criterion for probiotic. Among the isolates Lactobacillus casei (MG573073) was found to be highly tolerant of low pH and bile salts, posed strongest cell surface hydrophobicity of 75%. However, the maximum zone of inhibition was observed against Amoxilline/clavunic acid 44mm. The cell growth was found higher in presence of 2% inulin with cell viability 9.11 log 10 CFU/ml. In conclusion, based on the obtained results, Lactobacillus casei can act as a suitable probiotic candidate.

Autecology of scum producing bacteria

1998 ◽  
Vol 37 (4-5) ◽  
pp. 527-530 ◽  
Author(s):  
Hilde Lemmer ◽  
George Lind ◽  
Margit Schade ◽  
Birgit Ziegelmayer
Keyword(s):  
Wastewater Treatment ◽  
Cell Surface ◽  
Wastewater Treatment Plants ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Residence Times ◽  
Loading Conditions ◽  
Emulsifying Activity ◽  
Substrate Preferences

Non-filamentous hydrophobic scum bacteria were isolated from scumming wastewater treatment plants (WWTP) by means of adhesion to hydrocarbons. They were characterized with respect to taxonomy, substrate preferences, cell surface hydrophobicity, and emulsification capability. Their role during flotation events is discussed. Rhodococci are selected by hydrolysable substrates and contribute to flotation both by cell surface hydrophobicity and emulsifying activity at long mean cell residence times (MCRT). Saprophytic Acinetobacter strains are able to promote flotation by hydrophobicity and producing emulsifying agents under conditions when hydrophobic substrates are predominant. Hydrogenophaga and Acidovorax species as well as members of the Cytophaga/Flavobacterium group are prone to proliferate under low loading conditions and contribute to flotation mainly by emulsification.

Sign in / Sign up

Export Citation Format

Share Document