High-throughput process development methods for chromatography and precipitation of proteins: Advantages and precautions

2013 ◽  
Vol 13 (5) ◽  
pp. 446-455 ◽  
Author(s):  
Noriko Yoshimoto ◽  
Kazunobu Minakuchi ◽  
Daisuke Itoh ◽  
Yu Isakari ◽  
Shuichi Yamamoto
Keyword(s):  
High Throughput ◽  
Process Development ◽  
Development Methods

scholarly journals High-throughput microbioreactor provides a capable tool for early stage bioprocess development

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mathias Fink ◽  
Monika Cserjan-Puschmann ◽  
Daniela Reinisch ◽  
Gerald Striedner
Keyword(s):  
High Throughput ◽  
Process Development ◽  
Early Stage ◽  
Explanatory Power ◽  
Batch Mode ◽  
E Coli ◽  
Speed Up ◽  
Bioreactor System ◽  
Cell Densities ◽  
Microbial Systems

AbstractTremendous advancements in cell and protein engineering methodologies and bioinformatics have led to a vast increase in bacterial production clones and recombinant protein variants to be screened and evaluated. Consequently, an urgent need exists for efficient high-throughput (HTP) screening approaches to improve the efficiency in early process development as a basis to speed-up all subsequent steps in the course of process design and engineering. In this study, we selected the BioLector micro-bioreactor (µ-bioreactor) system as an HTP cultivation platform to screen E. coli expression clones producing representative protein candidates for biopharmaceutical applications. We evaluated the extent to which generated clones and condition screening results were transferable and comparable to results from fully controlled bioreactor systems operated in fed-batch mode at moderate or high cell densities. Direct comparison of 22 different production clones showed great transferability. We observed the same growth and expression characteristics, and identical clone rankings except one host-Fab-leader combination. This outcome demonstrates the explanatory power of HTP µ-bioreactor data and the suitability of this platform as a screening tool in upstream development of microbial systems. Fast, reliable, and transferable screening data significantly reduce experiments in fully controlled bioreactor systems and accelerate process development at lower cost.

scholarly journals Scale-down characterization of post-centrifuge flocculation processes for high-throughput process development

2014 ◽  
Vol 111 (12) ◽  
pp. 2486-2498 ◽  
Author(s):  
Georgina Espuny Garcia del Real ◽  
Jim Davies ◽  
Daniel G. Bracewell
Keyword(s):  
High Throughput ◽  
Process Development ◽  
Scale Down

Examination of a genetic algorithm for the application in high-throughput downstream process development

2012 ◽  
Vol 7 (10) ◽  
pp. 1203-1215 ◽  
Author(s):  
Katrin Treier ◽  
Annette Berg ◽  
Patrick Diederich ◽  
Katharina Lang ◽  
Anna Osberghaus ◽  
...  
Keyword(s):  
Genetic Algorithm ◽  
High Throughput ◽  
Process Development ◽  
Downstream Process

High-Throughput Automation in Chemical Process Development

2017 ◽  
Vol 8 (1) ◽  
pp. 525-547 ◽  
Author(s):  
Joshua A. Selekman ◽  
Jun Qiu ◽  
Kristy Tran ◽  
Jason Stevens ◽  
Victor Rosso ◽  
...  
Keyword(s):  
High Throughput ◽  
Chemical Process ◽  
Process Development

High-throughput process development for biopharmaceutical drug substances

2011 ◽  
Vol 29 (3) ◽  
pp. 127-135 ◽  
Author(s):  
Rahul Bhambure ◽  
Kaushal Kumar ◽  
Anurag S. Rathore
Keyword(s):  
High Throughput ◽  
Process Development ◽  
Drug Substances
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