Intergeneric yeast fusants with efficient ethanol production from cheese whey powder solution: Construction of aKluyveromyces marxianusandSaccharomyces cerevisiaeAY-5 hybrid

2012 ◽  
Vol 12 (6) ◽  
pp. 656-661 ◽  
Author(s):  
Xuewu Guo ◽  
Ruisheng Wang ◽  
Yefu Chen ◽  
Dongguang Xiao
Energies ◽  
2019 ◽  
Vol 12 (23) ◽  
pp. 4495 ◽  
Author(s):  
Csilla Farkas ◽  
Judit M. Rezessy-Szabó ◽  
Vijai Kumar Gupta ◽  
Erika Bujna ◽  
Tuan M. Pham ◽  
...  

Eight yeast strains of Lachancea thermotolerans, Kluyveromyces marxianus, and Kluyveromyces waltii have been tested for their ability to ferment lactose into ethanol in mashes containing 10% (w/v) cheese whey powder (CWP). The K. marxianus NCAIM Y00963 achieved 3.5% (v/v) ethanol concentration at 96–120 h of fermentation. The ethanol production by the selected lactose-positive strains and the well-known ethanologenic Saccharomyces cerevisiae (Levuline Fb) in mixed culture was also investigated at different CWP concentrations and inoculation techniques in batch mode. The mixed culture in an equal ratio (1:1) of cell counts of K. marxianus and S. serevisiae showed an increase in lactose conversion rate. The two yeast strains in a ratio of 3:1 (three-quarters of K. marxianus and a quarter of S. cerevisiae in a total of 4.5 × 1010 cells) resulted in 72.33% efficiency of lactose bioconversion and 7.6% (v/v) ethanol production at 17.5% (w/v) of CWP concentration. In the repeated inoculation process, with the addition of three-quarter part of 3:1 ratio of mixed culture (3.3 × 1010 cells of K. marxianus) into 150 mL CWP mash at initiation and the rest quarter part (1.2 × 1010 cells of S. cerevisiae) at 24 h, 8.86% (v/v) ethanol content with 87.5% efficiency of lactose conversion was reached. Both the ethanol concentration and efficiency of bioconversion were increased to 10.34% (v/v) and 92%, respectively, by combination with fed-batch fermentation technology. Our results can serve a very good basis for the development of industrial technology for the utilization of cheese whey.


2011 ◽  
Vol 35 (5) ◽  
pp. 1977-1982 ◽  
Author(s):  
Giuliano Dragone ◽  
Solange I. Mussatto ◽  
João B. Almeida e Silva ◽  
José A. Teixeira

2019 ◽  
Vol 67 (3) ◽  
pp. 829-835 ◽  
Author(s):  
Zhaojuan Zheng ◽  
Jiaxiao Xie ◽  
Peng Liu ◽  
Xin Li ◽  
Jia Ouyang
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