Synthesis of Poly(3-hydroxybutyrate-co-4-hydrobutyrate) with a Target Mole Fraction of 4-Hydroxybutyric Acid Units by Two-Stage Continuous Cultivation ofDelftia acidovorans P4a

2005 ◽  
Vol 5 (1) ◽  
pp. 58-62 ◽  
Author(s):  
G. Mothes ◽  
J.-U. Ackermann
Keyword(s):  
Mole Fraction ◽  
Continuous Cultivation ◽  
Hydroxybutyric Acid ◽  
Two Stage

Production of poly-β-hydroxybutyric acid by microorganisms accumulated from river water using a two-stage perfusion culture system

2000 ◽  
Vol 89 (1) ◽  
pp. 97-99 ◽  
Author(s):  
Toshiaki Morimoto ◽  
Fumio Tashiro ◽  
Hideyuki Nagashima ◽  
Kaori Nishizawa ◽  
Fukue Nagata ◽  
...  
Keyword(s):  
River Water ◽  
Culture System ◽  
Perfusion Culture ◽  
Hydroxybutyric Acid ◽  
Two Stage

Fermentative production of poly(β-hydroxybutyric acid) from xylose via L-lactate by a two-stage culture method employing Lactococcus lactis IO-1 and Alcaligenes eutrophus

1995 ◽  
Vol 41 (13) ◽  
pp. 257-261 ◽  
Author(s):  
Kenji Tanaka ◽  
Kouji Katamune ◽  
Ayaaki Ishizaki
Keyword(s):  
Growth Rate ◽  
Lactic Acid ◽  
Lactococcus Lactis ◽  
Batch Culture ◽  
Lactate Concentration ◽  
Culture Method ◽  
Alcaligenes Eutrophus ◽  
Hydroxybutyric Acid ◽  
Two Stage ◽  
Two Stage Culture

A two-stage culture method employing Lactococcus lactis IO-1 and Alcaligenes eutrophus was developed for the production of polyhydroxyalkanoic acid (PHA) from xylose via L-lactate. In this culture method, xylose was converted into L-lactic acid and acetic acid by a culture of L. lactis IO-1, and the organic acids were then converted into PHA by a culture of A. eutrophus. Alcaligenes eutrophus grew at a specific growth rate of 0.61/h; however, the growth rate decreased as the lactate concentration in the medium increased. When the supernatant of the IO-1 culture broth containing 10-g/L L-lactate was used as a medium for A. eutrophus in batch culture, the cell concentration increased to 8.5 g/L in 24 h and 55% (w/w) of the content of the cells was found to be poly(β-hydroxybutyric acid), P(3HB). Furthermore, fed-batch culture of A. eutrophus was carried out with feeding of L-lactic acid to maintain the L-lactate concentration around 3.0 g/L. As a result, 41.0 g/L of cells and 28.7 g/L of P(3HB) were produced after 17 h of cultivation.Key words: polyhydroxybutyric acid, xylose, L-lactic acid, Alcaligenes eutrophus, Lactococcus lactis.

Two-stage self-seeding method for preparation of single crystals of poly(phenylene sulfide)

1989 ◽  
Vol 47 ◽  
pp. 368-369
Author(s):  
Sengshiu Chung ◽  
Peggy Cebe
Keyword(s):  
Single Crystals ◽  
High Performance ◽  
Dilute Solution ◽  
Two Stage ◽  
Annealing Behavior ◽  
High Performance Polymers ◽  
Seeding Method ◽  
Phenylene Sulfide

We are studying the crystallization and annealing behavior of high performance polymers, like poly(p-pheny1ene sulfide) PPS, and poly-(etheretherketone), PEEK. Our purpose is to determine whether PPS, which is similar in many ways to PEEK, undergoes reorganization during annealing. In an effort to address the issue of reorganization, we are studying solution grown single crystals of PPS as model materials.Observation of solution grown PPS crystals has been reported. Even from dilute solution, embrionic spherulites and aggregates were formed. We observe that these morphologies result when solutions containing uncrystallized polymer are cooled. To obtain samples of uniform single crystals, we have used two-stage self seeding and solution replacement techniques.

364: Two stage "Progressive Elongation of Spermatic Cord" Technique in Criptorchidism with very short Spermatic Vessels

2007 ◽  
Vol 177 (4S) ◽  
pp. 121-121
Author(s):  
Antonio Dessanti ◽  
Diego Falchetti ◽  
Marco Iannuccelli ◽  
Susanna Milianti ◽  
Gian P. Strusi ◽  
...  
Keyword(s):  
Spermatic Cord ◽  
Two Stage
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