Front Cover: Discrimination of single‐stranded DNA homopolymers by sieving out G‐quadruplex using tiny solid‐state nanopores

2019 ◽  
Vol 40 (16-17) ◽  
Author(s):  
Wei Si ◽  
Haojie Yang ◽  
Jingjie Sha ◽  
Yin Zhang ◽  
Yunfei Chen
Keyword(s):  
Solid State ◽  
Single Stranded Dna ◽  
Front Cover ◽  
G Quadruplex

Discrimination of single‐stranded DNA homopolymers by sieving out G‐quadruplex using tiny solid‐state nanopores

2019 ◽  
Vol 40 (16-17) ◽  
pp. 2117-2124 ◽  
Author(s):  
Wei Si ◽  
Haojie Yang ◽  
Jingjie Sha ◽  
Yin Zhang ◽  
Yunfei Chen
Keyword(s):  
Solid State ◽  
Single Stranded Dna ◽  
G Quadruplex

scholarly journals Monitoring G-quadruplex formation with DNA carriers and solid-state nanopores

2019 ◽  
Author(s):  
Filip Bošković ◽  
Jinbo Zhu ◽  
Kaikai Chen ◽  
Ulrich F. Keyser
Keyword(s):  
Solid State ◽  
Single Molecule ◽  
Drug Targets ◽  
Gene Expression Regulation ◽  
Single Stranded Dna ◽  
Single Molecule Level ◽  
G Quadruplex ◽  
Human Viruses ◽  
Kinetics Of ◽  
Dna Carriers

ABSTRACTG-quadruplexes (Gq) are guanine-rich DNA structures formed by single-stranded DNA. They are of paramount significance to gene expression regulation, but also drug targets for cancer and human viruses. Current ensemble and single-molecule methods require fluorescent labels, which can affect Gq folding kinetics. Here we introduce, a single-molecule Gq nanopore assay (smGNA) to detect Gqs and kinetics of Gq formation. We use ~5 nm solid-state nanopores to detect various Gq structural variants attached to designed DNA carriers. Gqs can be identified by localizing their positions along designed DNA carriers establishing smGNA as a tool for Gq mapping. In addition, smGNA allows for discrimination of (un-)folded Gq structures, provides insights into single-molecule kinetics of G-quadruplex folding, and probes quadruplex-to-duplex structural transitions. smGNA can elucidate the formation of G-quadruplexes at the single-molecule level without labelling and has potential implications on the study of these structures both in single-stranded DNA and in genomic samples.

scholarly journals Discrimination of three types of homopolymers in single-stranded DNA with solid-state nanopores through external control of the DNA motion

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Rena Akahori ◽  
Itaru Yanagi ◽  
Yusuke Goto ◽  
Kunio Harada ◽  
Takahide Yokoi ◽  
...  
Keyword(s):  
Solid State ◽  
External Control ◽  
Single Stranded Dna ◽  
Dna Motion

Front Cover: Stiffness measurement of nanosized liposomes using solid‐state nanopore sensor with automated recapturing platform

2019 ◽  
Vol 40 (9) ◽  
Author(s):  
Jung Soo Lee ◽  
Jugal Saharia ◽  
Y. M. Nuwan D. Y. Bandara ◽  
Buddini Iroshika Karawdeniya ◽  
Gaurav Goyal ◽  
...  
Keyword(s):  
Solid State ◽  
Front Cover ◽  
Stiffness Measurement

scholarly journals Detecting Single Stranded DNA with a Solid State Nanopore

Nano Letters ◽  
2005 ◽  
Vol 5 (10) ◽  
pp. 1905-1909 ◽  
Author(s):  
Daniel Fologea ◽  
Marc Gershow ◽  
Bradley Ledden ◽  
David S. McNabb ◽  
Jene A. Golovchenko ◽  
...  
Keyword(s):  
Solid State ◽  
Single Stranded Dna

scholarly journals Human Origin Recognition Complex Binds Preferentially to G-quadruplex-preferable RNA and Single-stranded DNA

2013 ◽  
Vol 288 (42) ◽  
pp. 30161-30171 ◽  
Author(s):  
Shoko Hoshina ◽  
Kei Yura ◽  
Honami Teranishi ◽  
Noriko Kiyasu ◽  
Ayumi Tominaga ◽  
...  
Keyword(s):  
Origin Recognition Complex ◽  
Human Origin ◽  
Single Stranded Dna ◽  
G Quadruplex ◽  
Origin Recognition

scholarly journals Common Aspects of G-Quadruplex Destabilization among Helicases and Single Stranded DNA Binding Proteins

2015 ◽  
Vol 108 (2) ◽  
pp. 399a-400a
Author(s):  
Jagat B. Budhathoki ◽  
Sujay Ray ◽  
Pavel Janscak ◽  
Jaya Yodh ◽  
Hamza Balci
Keyword(s):  
Dna Binding ◽  
Binding Proteins ◽  
Dna Binding Proteins ◽  
Single Stranded Dna ◽  
G Quadruplex
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