Optimization of background electrolyte composition for simultaneous contactless conductivity and fluorescence detection in capillary electrophoresis of biological samples

2019 ◽  
Author(s):  
Júlia Lačná ◽  
Jan Přikryl ◽  
Norio Teshima ◽  
Hiroya Murakami ◽  
Yukihiro Esaka ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Fluorescence Detection ◽  
Biological Samples ◽  
Background Electrolyte ◽  
Electrolyte Composition

scholarly journals Optimisation of analytical conditions for simultaneous determination of phenol and chlorophenols by capillary electrophoresis with indirect laser-induced fluorescence detection

2021 ◽  
Vol 63 (11) ◽  
pp. 51-55
Author(s):  
Manh Huy Nguyen ◽  
◽  
Hong Anh Duong ◽  
Hung Viet Pham ◽  
◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Phenolic Compounds ◽  
Simultaneous Determination ◽  
Fluorescence Detection ◽  
Background Electrolyte ◽  
Laser Induced Fluorescence ◽  
Laser Induced Fluorescence Detection ◽  
Electrophoresis Method ◽  
Wastewater Samples

This work presented a capillary electrophoresis method with indirect laser-induced fluorescence detection for the simultaneous determination of phenol and four chlorophenol derivatives. The separation was obtained within 20 minutes with a background electrolyte composed of 5 mM borax and 1 mM fluorescein (pH=9.75), 17 kV of applied voltage, and 120 s of hydrostatic injection. At the optimal conditions, the limit of detections for phenolic compounds was in the range of 0.08-0.23 mg/l, and the RSD data of repeatability and reproducibility were less than 8.0% for both migration times and peak areas. This developed method was applied to analyse concentrations of phenolic compounds in surface water and wastewater samples, with the recoveries ranging from 59.4 to 102.5%.

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