Rapid and sensitive double-label based immunochromatographic assay for zearalenone detection in cereals

2018 ◽  
Vol 39 (16) ◽  
pp. 2125-2130 ◽  
Author(s):  
Du Wang ◽  
Zhaowei Zhang ◽  
Qi Zhang ◽  
Zhongzheng Wang ◽  
Wen Zhang ◽  
...  
Keyword(s):  
Immunochromatographic Assay ◽  
Double Label

Development of a monoclonal antibody-based immunochromatographic strip for the rapid detection of tigecycline in human serum

2021 ◽  
Author(s):  
Chuanlai Xu ◽  
qianqian lu ◽  
Xin-Xin Xu ◽  
Shanshan Song ◽  
aihong wu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Human Serum ◽  
Rapid Detection ◽  
Immunochromatographic Assay ◽  
Immunochromatographic Strip

In this study, a gold labelled immunochromatographic assay was developed to detect tigecycline (TGC) in human serum. For this purpose, an anti-TGC monoclonal antibody, 2G7, was produced and characterized, and...

scholarly journals The evaluation of a novel digital immunochromatographic assay with silver amplification to detect SARS-CoV-2

2021 ◽  
Author(s):  
Yoko Kurihara ◽  
Yoshihiko Kiyasu ◽  
Yusaku Akashi ◽  
Yuto Takeuchi ◽  
Kenji Narahara ◽  
...  
Keyword(s):  
Immunochromatographic Assay ◽  
Silver Amplification

scholarly journals Development of a Colloidal Gold Immunochromatographic Assay for Duck Enteritis Virus Detection Using Monoclonal Antibodies

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 365
Author(s):  
Fengli Liu ◽  
Yanxin Cao ◽  
Maokai Yan ◽  
Mengxu Sun ◽  
Qingshui Zhang ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Colloidal Gold ◽  
Preventive Intervention ◽  
Virus Detection ◽  
Cross Reactivity ◽  
Duck Enteritis Virus ◽  
Detection Sensitivity ◽  
Immunochromatographic Assay ◽  
Efficient Detection ◽  
Enteritis Virus

Duck viral enteritis is a highly contagious and fatal disease of commercial waterfowl flocks. The disease occurs sporadically or epizootically in mainland China due to insufficient vaccinations. Early and rapid diagnosis is important for preventive intervention and the control of epizootic events in clinical settings. In this study, we generated two monoclonal antibodies (MAbs) that specifically recognized the duck enteritis virus (DEV) envelope glycoprotein B and tegument protein UL47, respectively. Using these MAbs, a colloidal gold-based immunochromatographic assay (ICA) was developed for the efficient detection of DEV antigens within 15 min. Our results showed that the detection limit of the developed ICA strip was 2.52 × 103 TCID50/mL for the virus infected cell culture suspension with no cross-reactivity with other pathogenic viruses commonly encountered in commercially raised waterfowl. Using samples from experimentally infected ducks, we demonstrated that the ICA detected the virus in cloacal swab samples on day three post-infection, demonstrating an 80% concordance with the PCR. For tissue homogenates from ducks succumbing to infection, the detection sensitivity was 100%. The efficient and specific detection by this ICA test provides a valuable, convenient, easy to use and rapid diagnostic tool for DVE under both laboratory and field conditions.

scholarly journals Simultaneous detection of diethylstilbestrol and estradiol residues with a single immunochromatographic assay strip

2021 ◽  
Author(s):  
Zehua Xu ◽  
Tieqiang Sun ◽  
Hongwei He ◽  
Wentao Liu ◽  
Longxing Fan ◽  
...  
Keyword(s):  
Simultaneous Detection ◽  
Immunochromatographic Assay

Chrysanthemum-like Au@Polydopamine Synthesized using One-Pot Method and Its Advantage in Immunochromatographic Assay

2021 ◽  
pp. 130097
Author(s):  
Gang-gang Zhang ◽  
Xiao-cui Lai ◽  
Neng-shui Ding ◽  
Qi-rong Xiong ◽  
Shuai Hou ◽  
...  
Keyword(s):  
Immunochromatographic Assay ◽  
One Pot

Immunochromatographic assay of T-2 toxin using labeled anti-species antibodies

2017 ◽  
Vol 53 (5) ◽  
pp. 594-599 ◽  
Author(s):  
A. E. Urusov ◽  
A. V. Petrakova ◽  
A. V. Bartosh ◽  
M. K. Gubaydullina ◽  
A. V. Zherdev ◽  
...  
Keyword(s):  
Immunochromatographic Assay
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