Microscale isolation of native forms of lysozyme from chicken egg white by gel isoelectric focusing

2018 ◽  
Vol 39 (8) ◽  
pp. 1054-1061 ◽  
Author(s):  
Youji Shimazaki ◽  
Yoshiko Ochi ◽  
Kennosuke Fujimura
Keyword(s):  
Isoelectric Focusing ◽  
Egg White ◽  
Chicken Egg ◽  
Gel Isoelectric Focusing

Separation of Ovotransferrin and Ovomucoid from Chicken Egg White

2016 ◽  
Author(s):  
Sandun Abeyrathne ◽  
Hyunyong Lee ◽  
Dong U. Ahn
Keyword(s):  
Egg White ◽  
Chicken Egg

scholarly journals Unconventional effects of long-term storage of microwave-modified chicken egg white lysozyme preparations

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Grzegorz Leśnierowski ◽  
Tianyu Yang ◽  
Renata Cegielska-Radziejewska
Keyword(s):  
Microwave Radiation ◽  
Egg White ◽  
Process Time ◽  
High Quality ◽  
Chicken Egg ◽  
Egg White Lysozyme ◽  
Physicochemical Changes ◽  
Long Term Storage ◽  
Term Storage

AbstractThermal modification is an effective method that induces significant expansion of the antimicrobial properties and other valuable properties of chicken egg white lysozyme. In our latest research, a new innovative method of enzyme modification was developed, in which microwave radiation was used as an energy source to process liquid lysozyme concentrate (LLC). After modification, high-quality preparations were obtained. However, long-term storage in a concentrated form initiated various processes that caused darkening over time and could also lead to other significant changes to their structure and, consequently, to their functional properties. This necessitated multidirectional research to explain this phenomenon. This paper presents the results of research aimed at assessing the physicochemical changes in the properties of microwave-modified lysozyme in the form of a liquid concentrate after long-term storage under refrigeration conditions. The assessment also considered the conditions under the acidity of the modifying medium and the duration of the microwave modification. The analysis showed that the values of the basic parameters determining the quality and usefulness of the modified enzyme significantly improved during long-term storage of the preparations. The greatest changes were observed in the preparations modified for the longest time and in the most acidic environment (process time 260 s, pH 2.0), the number of oligomers under these conditions increased by 18% after 12 months of holding, and the surface hydrophobicity increased by as much as 31%. In addition, microbiological tests showed that the preparations of microwave-modified lysozyme had an effect on gram-positive bacteria as well as on gram-negative, and this effect was significantly enhanced after 12 months. The results confirm that LLC modification with microwave radiation is a highly efficient method to prepare high-quality and high utility potential lysozyme. Notably, an interesting and important phenomenon was the observation of the unconventional behaviour of the preparations during their long-term storage, which increased their utility potential significantly.

The effect of solution thermal history on chicken egg white lysozyme nucleation

2001 ◽  
Vol 232 (1-4) ◽  
pp. 301-307 ◽  
Author(s):  
Michael W. Burke ◽  
Russell A. Judge ◽  
Marc L. Pusey
Keyword(s):  
Thermal History ◽  
Egg White ◽  
Chicken Egg ◽  
Egg White Lysozyme

Use of Magnetic Fe3O4 Nanoparticles Coated with Bovine Serum Albumin for the Separation OF Lysozyme from Chicken Egg white

2021 ◽  
pp. 129442
Author(s):  
Mariane Gonçalves Santos ◽  
Diailison Teixeira de Carvalho ◽  
Lucas Belga Caminitti ◽  
Bruna Bueno Alves de Lima ◽  
Marcello Henrique da Silva Cavalcanti ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Fe3o4 Nanoparticles ◽  
Bovine Serum ◽  
Egg White ◽  
Chicken Egg ◽  
Magnetic Fe3o4 Nanoparticles ◽  
Magnetic Fe3o4
Sign in / Sign up

Export Citation Format

Share Document