Enhanced cell trapping throughput using DC-biased AC electric field in a dielectrophoresis-based fluidic device with densely packed silica beads

2018 ◽  
Vol 39 (5-6) ◽  
pp. 878-886 ◽  
Author(s):  
Nuttawut Lewpiriyawong ◽  
Guolin Xu ◽  
Chun Yang
Keyword(s):  
Electric Field ◽  
Cell Trapping ◽  
Ac Electric Field ◽  
Silica Beads ◽  
Fluidic Device

Contactless cell trapping by the use of a uniform AC electric field

Biorheology ◽  
2013 ◽  
Vol 50 (5-6) ◽  
pp. 283-303 ◽  
Author(s):  
Shigeru Tada ◽  
Tomoyuki Natsuya ◽  
Akira Tsukamoto ◽  
Yudai Santo
Keyword(s):  
Electric Field ◽  
Cell Trapping ◽  
Ac Electric Field

Line Patterning with Microparticles at Different Positions in a Single Device Based on Negative Dielectrophoresis

2013 ◽  
Vol 25 (4) ◽  
pp. 650-656 ◽  
Author(s):  
Tomoyuki Yasukawa ◽  
◽  
Yusuke Yoshida ◽  
Hironobu Hatanaka ◽  
Fumio Mizutani
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
Indium Tin Oxide ◽  
Strong Electric Field ◽  
Electric Signal ◽  
Electric Field Line ◽  
Peak Voltage ◽  
Ac Electric Field ◽  
Fluidic Device ◽  
Line Patterns

We report on control of line pattern positioning with particles fabricated by negative dielectrophoresis (n-DEP) using the applied intensity and phase of an AC electric field. Line patterns were fabricated in a microfluidic device consisting of upper conductive indium-tin-oxide (ITO) substrates and lower ITOinterdigitated microband array (IDA) electrodes used as the template. A 6-µm-diameter polystyrene particles suspension was introduced into the device between upper ITO and the bottom ITO-IDA substrate. An AC electric signal of a typically 20 peak-to-peak voltage and 1.0 MHz was then applied to upper ITO and bands on lower IDA, resulting in the formation of line patterns with low electric-field gradient regions. AC voltage was applied to bands A and B on lower IDA with the opposite phase and the same frequency and intensity. When the signal identical to band A was applied to upper ITO, particles were aligned above band A because relatively lower electric fields were produced in these regions. In contrast, the application of a signal identical to band B formed line patterns with particles aligned above band B due to the generation of a strong electric field between band A and upper ITO and the disappearance of the strong electric field between band B and upper ITO. The decrease in applied intensity to upper ITO shifted the accumulated position of particles to the center between bands A and B because of the balance of electric fields generated between band A or B and upper ITO. We thus fabricated line patterns with particles at desired positions in the fluidic device.

Simulation of TiO 2 particle trajectory in AC electric field

2015 ◽  
Vol 108 ◽  
pp. 183-191 ◽  
Author(s):  
Reza Riahifar ◽  
Babak Raissi ◽  
Cyrus Zamani ◽  
Ehsan Marzbanrad
Keyword(s):  
Electric Field ◽  
Particle Trajectory ◽  
Ac Electric Field

Electrically induced reversible viscosity change in hectorite aqueous dispersion under an AC electric field

2014 ◽  
Vol 99 ◽  
pp. 160-163 ◽  
Author(s):  
Hiroshi Kimura ◽  
Mao Ueno ◽  
Shinya Takahashi ◽  
Akira Tsuchida ◽  
Keiichi Kurosaka
Keyword(s):  
Electric Field ◽  
Aqueous Dispersion ◽  
Viscosity Change ◽  
Ac Electric Field

Mechanism of Cell Lysis in Microfluidic Channel With Integrated Nanocomposite Electrodes

2013 ◽  
Author(s):  
Madhusmita Mishra ◽  
Anil Krishna Koduri ◽  
Aman Chandra ◽  
D. Roy Mahapatra ◽  
G. M. Hegde
Keyword(s):  
Electric Field ◽  
Microfluidic Channel ◽  
Cell Lysis ◽  
Bacterial Cells ◽  
Nano Composite ◽  
Time Resolved ◽  
Ac Electric Field ◽  
Cell Dispersion ◽  
Electrical Lysis ◽  
Nanocomposite Electrodes

This paper reports on the characterization of an integrated micro-fluidic platform for controlled electrical lysis of biological cells and subsequent extraction of intracellular biomolecules. The proposed methodology is capable of high throughput electrical cell lysis facilitated by nano-composite coated electrodes. The nano-composites are synthesized using Carbon Nanotube and ZnO nanorod dispersion in polymer. Bacterial cells are used to demonstrate the lysis performance of these nanocomposite electrodes. Investigation of electrical lysis in the microchannel is carried out under different parameters, one with continuous DC application and the other under DC biased AC electric field. Lysis in DC field is dependent on optimal field strength and governed by the cell type. By introducing the AC electrical field, the electrokinetics is controlled to prevent cell clogging in the micro-channel and ensure uniform cell dispersion and lysis. Lysis mechanism is analyzed with time-resolved fluorescence imaging which reveal the time scale of electrical lysis and explain the dynamic behavior of GFP-expressing E. coli cells under the electric field induced by nanocomposite electrodes. The DNA and protein samples extracted after lysis are compared with those obtained from a conventional chemical lysis method by using a UV–Visible spectroscopy and fluorimetry. The paper also focuses on the mechanistic understanding of the nano-composite coating material and the film thickness on the leakage charge densities which lead to differential lysis efficiency.

Deformation of liquid crystal droplets under the action of an external ac electric field

2001 ◽  
Vol 64 (2) ◽  
Author(s):  
B. I. Lev ◽  
V. G. Nazarenko ◽  
A. B. Nych ◽  
D. Schur ◽  
P. M. Tomchuk ◽  
...  
Keyword(s):  
Liquid Crystal ◽  
Electric Field ◽  
Ac Electric Field

Electrostatic Dispensing of Dry Dielectric Materials

2001 ◽  
Vol 700 ◽  
Author(s):  
Malinda M. Tupper ◽  
Marjorie E. Chopinaud ◽  
Takamichi Ogawa ◽  
Michael J. Cima
Keyword(s):  
Electric Field ◽  
Field Intensity ◽  
Electric Field Intensity ◽  
Dielectric Materials ◽  
Nonuniform Electric Field ◽  
Sodium Fluorescein ◽  
Silica Spheres ◽  
Electrode Geometry ◽  
Wide Range ◽  
Silica Beads

AbstractDispensing micron-scale dielectric materials can be achieved through the use of dielectrophoresis. Electrodes are designed to create a nonuniform electric field. This method is expected to be applicable for transfer of a wide range of dielectric powders as well as small, shaped components. Small, 150 μm diameter silica spheres, as well as sodium fluorescein powder have been dispensed by this method. Selecting the appropriate electrode geometry and electric field intensity controls the amount collected. As little as 1.0 μg of sodium fluorescein powder, and as much as 16 mg of silica beads have been collected, and repeatability within 10 % of the total amount dispensed has been achieved.

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