Enhanced sensitivity and resolution for the analysis of paralytic shellfish poisoning toxins in water using capillary electrophoresis with amperometric detection and field-amplified sample injection

2016 ◽  
Vol 37 (23-24) ◽  
pp. 3109-3117 ◽  
Author(s):  
Minsheng Li ◽  
Xiaoyan Chen ◽  
Yuan Guo ◽  
Bingyu Zhang ◽  
Fengxiang Tang ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Amperometric Detection ◽  
Paralytic Shellfish Poisoning ◽  
Shellfish Poisoning ◽  
Sample Injection ◽  
Enhanced Sensitivity ◽  
Paralytic Shellfish

Capillary electrophoresis for the analysis of paralytic shellfish poisoning toxins in shellfish: Comparison of detection methods

2014 ◽  
Vol 35 (10) ◽  
pp. 1496-1503 ◽  
Author(s):  
Aemi S. Abdul Keyon ◽  
Rosanne M. Guijt ◽  
Andras Gaspar ◽  
Artaches A. Kazarian ◽  
Pavel N. Nesterenko ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Paralytic Shellfish Poisoning ◽  
Detection Methods ◽  
Shellfish Poisoning ◽  
Paralytic Shellfish

Assessment of a Semiquantitative Liquid Chromatography- Fluorescence Detection Method for the Determination of Paralytic Shellfish Poisoning Toxin Levels in Bivalve Molluscs from Great Britain

2014 ◽  
Vol 97 (2) ◽  
pp. 492-497 ◽  
Author(s):  
Andrew D Turner ◽  
Monika Dhanji-Rapkova ◽  
Clothilde Baker ◽  
Myriam Algoet
Keyword(s):  
Great Britain ◽  
Fluorescence Detection ◽  
Detection Method ◽  
Reference Method ◽  
Paralytic Shellfish Poisoning ◽  
Official Method ◽  
Shellfish Poisoning ◽  
Bivalve Molluscs ◽  
Paralytic Shellfish

Abstract AOAC Official Method 2005.06 precolumn oxidation LC-fluorescence detection method has been used for many years for the detection and quantitation of paralytic shellfish poisoning (PSP) toxins in bivalve molluscs. After extensive single- and multiple-laboratory validation, the method has been slowly gaining acceptance worldwide as a useful and practical tool for official control testing. In Great Britain, the method has become routine since 2008, with no requirement since then for reverting back to the bioassay reference method. Although the method has been refined to be semiautomated, faster, and more reproducible, the quantitation step can be complex and time-consuming. An alternative approach was developed to utilize the qualitative screening results for generatinga semiquantitative results assessment. Data obtained over 5 years enabled the comparison of semiquantitative and fully quantitative PSP results in over 15 000 shellfish samples comprising eight different species showed that the semiquantitative approach resulted in over-estimated paralytic shellfish toxin levels by an average factor close to two in comparison with the fully quantified levels. No temporal trends were observed in the data or relating to species type, with the exception of surf clams. The comparison suggested a semiquantitative threshold of 800 μg saxitoxin (STX) eq/kg should provide a safe limitfor the determination of samples to be forwarded to full quantitation. However, the decision was taken to halve this limit to include an additional safety factor of 2, resulting in the use of a semiquantitative threshold of 400 μg STX eq/kg. Implementation of the semiquantitative method into routine testing would result in a significant reduction in the numbers of samples requiring quantitation and have a positive impact on the overall turnaround of reported PSP results. The refined method would be appropriate for any monitoring laboratory faced with high throughput requirements.

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