Highly sensitive detection of cancer cells based on the DNA barcode assay and microcapillary electrophoretic analysis

2014 ◽  
Vol 35 (10) ◽  
pp. NA-NA
Author(s):  
Soyi Chung ◽  
Minkyung Cho ◽  
Jae Hwan Jung ◽  
Tae Seok Seo
Keyword(s):  
Cancer Cells ◽  
Dna Barcode ◽  
Electrophoretic Analysis ◽  
Sensitive Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

scholarly journals Highly sensitive detection of cancer cells using femtosecond dual-wavelength near-IR two-photon imaging

2012 ◽  
Vol 3 (7) ◽  
pp. 1534 ◽  
Author(s):  
Jean R. Starkey ◽  
Nikolay S. Makarov ◽  
Mikhail Drobizhev ◽  
Aleksander Rebane
Keyword(s):  
Cancer Cells ◽  
Dual Wavelength ◽  
Sensitive Detection ◽  
Two Photon ◽  
Near Ir ◽  
Highly Sensitive ◽  
Photon Imaging ◽  
Two Photon Imaging ◽  
Highly Sensitive Detection

scholarly journals Aptamer Embedded Arch-Cruciform DNA Assemblies on 2-D VS2 Scaffolds for Sensitive Detection of Breast Cancer Cells

Biosensors ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 378
Author(s):  
Jinfeng Quan ◽  
Yihan Wang ◽  
Jialei Zhang ◽  
Kejing Huang ◽  
Xuemei Wang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Dna Sequences ◽  
Breast Cancer Cells ◽  
Limit Of Detection ◽  
Sensitive Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Cruciform Dna ◽  
Mcf 7

Arch-cruciform DNA are self-assembled on AuNPs/VS2 scaffold as a highly sensitive and selective electrochemical biosensor for michigan cancer foundation-7 (MCF-7) breast cancer cells. In the construction, arch DNA is formed using two single-strand DNA sequences embedded with the aptamer for MCF-7 cells. In the absence of MCF-7 cells, a cruciform DNA labeled with three terminal biotin is bound to the top of arch DNA, which further combines with streptavidin-labeled horseradish peroxidase (HRP) to catalyze the hydroquinone-H2O2 reaction on the electrode surface. The presence of MCF-7 cells can release the cruciform DNA and reduce the amount of immobilized HRP, thus effectively inhibiting enzyme-mediated electrocatalysis. The electrochemical response of the sensor is negatively correlated with the concentration of MCF-7 cells, with a linear range of 10 − 1 × 105 cells/mL, and a limit of detection as low as 5 cells/mL (S/N = 3). Through two-dimensional materials and enzyme-based dual signal amplification, this biosensor may pave new ways for the highly sensitive detection of tumor cells in real samples.

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