Determination of amino acids by microemulsion electrokinetic chromatography laser induced fluorescence method

2014 ◽  
Vol 35 (11) ◽  
pp. 1751-1755 ◽  
Author(s):  
Wei-Cheng Lin ◽  
Wan-Ling Liu ◽  
Wei-Ying Tang ◽  
Chin-Ping Huang ◽  
Hsi-Ya Huang ◽  
...  
Keyword(s):  
Amino Acids ◽  
Laser Induced Fluorescence ◽  
Fluorescence Method ◽  
Electrokinetic Chromatography ◽  
Microemulsion Electrokinetic Chromatography

scholarly journals Analysis of Seven Biogenic Amines and Two Amino Acids in Wines Using Micellar Electrokinetic Chromatography

2019 ◽  
Vol 9 (6) ◽  
pp. 1193 ◽  
Author(s):  
Chih-Ying Huang ◽  
Yu-Xian Wang ◽  
Xin-Zhi Wang ◽  
Cho-Chun Hu ◽  
Tai-Chia Chiu
Keyword(s):  
Amino Acids ◽  
Biogenic Amines ◽  
Micellar Electrokinetic Chromatography ◽  
Low Cost ◽  
Background Electrolyte ◽  
Sodium Dodecyl ◽  
Fast Method ◽  
Electrokinetic Chromatography ◽  
Optimal Separation

A low-cost, simple, and fast method utilizing micellar electrokinetic chromatography for the simultaneous determination of seven biogenic amines and two amino acids was developed. A background electrolyte containing 5 mM phosphate buffer (pH 3.7) and 20 mM sodium dodecyl sulfate was used. The optimal separation of nine investigated analytes was achieved in 11 min, with limits of detection (S/N = 3) ranging from 0.11 to 0.61 µM. The linear ranges for all analytes were observed between 0.55 and 10.0 μM (R2 > 0.990). The developed approach was extended to the analysis of analytes in commercial wine and beer samples. The recoveries of the proposed method ranged from 98.8% to 115.6%.

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