A microfluidic chip-based fluorescent biosensor for the sensitive and specific detection of label-free single-base mismatch via magnetic beads-based “sandwich” hybridization strategy

2013 ◽  
Vol 34 (15) ◽  
pp. 2177-2184 ◽  
Author(s):  
ZongWen Wang ◽  
YingWei Fan ◽  
JinFa Chen ◽  
Ying Guo ◽  
WeiHua Wu ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Magnetic Beads ◽  
Label Free ◽  
Specific Detection ◽  
Single Base ◽  
Fluorescent Biosensor ◽  
Sandwich Hybridization ◽  
Single Base Mismatch ◽  
Base Mismatch

Label-free visual detection of nucleic acids in biological samples with single-base mismatch detection capability

2012 ◽  
Vol 48 (4) ◽  
pp. 576-578 ◽  
Author(s):  
Yanling Song ◽  
Weiting Zhang ◽  
Yuan An ◽  
Liang Cui ◽  
Chundong Yu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Biological Samples ◽  
Visual Detection ◽  
Label Free ◽  
Detection Capability ◽  
Single Base ◽  
Mismatch Detection ◽  
Single Base Mismatch ◽  
Base Mismatch

Simultaneous Discrimination of Single-Base Mismatch and Full Match Using a Label-Free Single-Molecule Strategy

2018 ◽  
Vol 90 (13) ◽  
pp. 8102-8107 ◽  
Author(s):  
Qiufang Yang ◽  
Tingting Ai ◽  
You Lv ◽  
Yuqin Huang ◽  
Jia Geng ◽  
...  
Keyword(s):  
Single Molecule ◽  
Simultaneous Discrimination ◽  
Label Free ◽  
Single Base ◽  
Single Base Mismatch ◽  
Base Mismatch

Aligner mediated cleavage of nucleic acids for site-specific detection of single base mismatch

Talanta ◽  
2019 ◽  
Vol 201 ◽  
pp. 358-363
Author(s):  
Wufan Pan ◽  
Dongdong Yu ◽  
Yazhou Qin ◽  
Wanghua Wu ◽  
Yuxiang Lu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Specific Detection ◽  
Site Specific ◽  
Single Base ◽  
Single Base Mismatch ◽  
Base Mismatch

Lanthanum-Based Metal–Organic Frameworks for Specific Detection of Sudan Virus RNA Conservative Sequences down to Single-Base Mismatch

2017 ◽  
Vol 56 (24) ◽  
pp. 14880-14887 ◽  
Author(s):  
Shui-Ping Yang ◽  
Wei Zhao ◽  
Pei-Pei Hu ◽  
Ke-Yang Wu ◽  
Zhi-Hong Jiang ◽  
...  
Keyword(s):  
Metal Organic Frameworks ◽  
Specific Detection ◽  
Single Base ◽  
Virus Rna ◽  
Single Base Mismatch ◽  
Base Mismatch ◽  
Metal Organic

Label-free detection of polynucleotide single-base mismatch via pyrene probe excimer emission

2011 ◽  
Vol 78 (2) ◽  
pp. 747-752 ◽  
Author(s):  
Dan Tang ◽  
Ping Lu ◽  
Dongli Liao ◽  
Xiangyu Yang ◽  
Yujing Zhang ◽  
...  
Keyword(s):  
Label Free ◽  
Excimer Emission ◽  
Single Base ◽  
Single Base Mismatch ◽  
Label Free Detection ◽  
Base Mismatch

Detection of p53 Gene Mutation (Single-Base Mismatch) Using a Fluorescent Silver Nanoclusters

2017 ◽  
Vol 27 (4) ◽  
pp. 1443-1448 ◽  
Author(s):  
Morteza Hosseini ◽  
Shiva Mohammadi ◽  
Yasaman-Sadat Borghei ◽  
Mohammad Reza Ganjali
Keyword(s):  
Gene Mutation ◽  
P53 Gene ◽  
Silver Nanoclusters ◽  
P53 Gene Mutation ◽  
Single Base ◽  
Single Base Mismatch ◽  
Base Mismatch

scholarly journals Digital-resolution detection of microRNA with single-base selectivity by photonic resonator absorption microscopy

2019 ◽  
Vol 116 (39) ◽  
pp. 19362-19367 ◽  
Author(s):  
Taylor D. Canady ◽  
Nantao Li ◽  
Lucas D. Smith ◽  
Yi Lu ◽  
Manish Kohli ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Signal To Noise Ratio ◽  
Resonant Wavelength ◽  
High Signal ◽  
Single Base ◽  
Reflected Light ◽  
Single Base Mismatch ◽  
Base Mismatch ◽  
Digital Resolution ◽  
Target Probe

Circulating exosomal microRNA (miR) represents a new class of blood-based biomarkers for cancer liquid biopsy. The detection of miR at a very low concentration and with single-base discrimination without the need for sophisticated equipment, large volumes, or elaborate sample processing is a challenge. To address this, we present an approach that is highly specific for a target miR sequence and has the ability to provide “digital” resolution of individual target molecules with high signal-to-noise ratio. Gold nanoparticle tags are prepared with thermodynamically optimized nucleic acid toehold probes that, when binding to a target miR sequence, displace a probe-protecting oligonucleotide and reveal a capture sequence that is used to selectively pull down the target-probe–nanoparticle complex to a photonic crystal (PC) biosensor surface. By matching the surface plasmon-resonant wavelength of the nanoparticle tag to the resonant wavelength of the PC nanostructure, the reflected light intensity from the PC is dramatically and locally quenched by the presence of each individual nanoparticle, enabling a form of biosensor microscopy that we call Photonic Resonator Absorption Microscopy (PRAM). Dynamic PRAM imaging of nanoparticle tag capture enables direct 100-aM limit of detection and single-base mismatch selectivity in a 2-h kinetic discrimination assay. The PRAM assay demonstrates that ultrasensitivity (<1 pM) and high selectivity can be achieved on a direct readout diagnostic.

Sign in / Sign up

Export Citation Format

Share Document