Better management of Western blotting results using professional photo management software

2013 ◽  
Vol 34 (8) ◽  
pp. 1219-1222
Author(s):  
Christian Iorio-Morin ◽  
Pascale Germain ◽  
Jean-Luc Parent
Keyword(s):  
Western Blotting ◽  
Management Software ◽  
Photo Management

Cross-Linked αsγt-Chain Hybrids in Plasma Clots Studied by 1D- and 2D Electrophoresis and Western Blotting

1993 ◽  
Vol 70 (03) ◽  
pp. 438-442 ◽  
Author(s):  
B Grøn ◽  
C Filion-Myklebust ◽  
S Bjørnsen ◽  
P Haidaris ◽  
F Brosstad
Keyword(s):  
Molecular Weight ◽  
Monoclonal Antibodies ◽  
Human Plasma ◽  
Western Blotting ◽  
Isoelectric Point ◽  
Factor Xiii ◽  
Cross Linking ◽  
2D Electrophoresis ◽  
Complex Phenomenon ◽  
Fibrinopeptide A

SummaryFibrinogen and fibrin related chains in reduced human plasma as well as the bonds interlinking partially cross-linked fibrin from plasma clots have been studied by means of 1D- and 2D electrophoresis and Western blotting. Immunovisualization of reduced plasma or partially cross-linked fibrin with monoclonal antibodies specific for the α-chains or the γ-chains have shown that several bands represent material belonging to both chains. In order to decide whether these bands constitute αγ-chain hybrids or superimposed α- and γ-chain dimers, the cross-linked material was separated according to both isoelectric point (pI) and molecular weight (MW) using Pharmacia’s Multiphor II system. Western blotting of the second dimension gels revealed that partially cross-linked fibrin contains αsγt-chain hybrids and γ- polymers, in addition to the well-known γ-dimers and α-polymers. The main αsγt-chain hybrid has a pI between that of the α- and the γ-chains, a MW of about 200 kDa and contains Aα-chains with intact fibrinopeptide A (FPA). It was also observed that soluble fibrinogen/fibrin complexes as well as partially cross-linked fibrin contain degraded α-dimers with MWs close to the γ-dimers. These findings demonstrate that factor XIII-catalyzed cross-linking of fibrin is a more complex phenomenon than earlier recognized.

Plasma Factor V Activation Is Prevented by Activated Protein C in the Presence of Phospholipid Vesicles, Not Platelets

1993 ◽  
Vol 69 (02) ◽  
pp. 124-129 ◽  
Author(s):  
Susan Solymoss ◽  
Kim Thi Phu Nguyen
Keyword(s):  
Western Blotting ◽  
Protein C ◽  
Thrombin Generation ◽  
Blood Platelets ◽  
Activated Protein C ◽  
Phospholipid Vesicles ◽  
Factor V ◽  
Two Stage ◽  
One Stage ◽  
Plasma Factor

SummaryActivated protein C (APC) is a vitamin K dependent anticoagulant which catalyzes the inactivation of factor Va and VIIIa, in a reaction modulated by phospholipid membrane surface, or blood platelets. APC prevents thrombin generation at a much lower concentration when added to recalcified plasma and phospholipid vesicles, than recalcified plasma and platelets. This observation was attributed to a platelet associated APC inhibitor. We have performed serial thrombin, factor V one stage and two stage assays and Western blotting of dilute recalcified plasma containing either phospholipid vesicles or platelets and APC. More thrombin was formed at a given APC concentration with platelets than phospholipid. One stage factor V values increased to higher levels with platelets and APC than phospholipid and APC. Two stage factor V values decreased substantially with platelets and 5 nM APC but remained unchanged with phospholipid and 5 nM APC. Western blotting of plasma factor V confirmed factor V activation in the presence of platelets and APC, but lack of factor V activation with phospholipid and APC. Inclusion of platelets or platelet membrane with phospholipid enhanced rather than inhibited APC catalyzed plasma factor V inactivation. Platelet activation further enhanced factor V activation and inactivation at any given APC concentration.Plasma thrombin generation in the presence of platelets and APC is related to ongoing factor V activation. No inhibition of APC inactivation of FVa occurs in the presence of platelets.

PENGELOLAAN DATA ANGGOTA BERBASIS WEBSITE DI YAMAHA VIXION CLUB CABANG BANDUNG

2020 ◽  
Vol 2 (2) ◽  
pp. 113-127
Author(s):  
Nova Indrayana Yusman
Keyword(s):  
Data Management ◽  
Organizational Structure ◽  
Human Resource Development ◽  
Human Resource ◽  
Programming Language ◽  
The Internet ◽  
Web Based ◽  
Management Software ◽  
The City ◽  
Data Management Software

Yamaha Vixion Club Bandung (YVCB) was formed on July 7, 2007 in the city of Bandung, as a place of friendship between Yamaha Vixion motorcyclists. In its organizational structure, YVCB has a Human Resource Development (HRD) division. Until now, there are more than 800 Yamaha Vixion Club Bandung members. This software is made to facilitate the work of the Yamaha Vixion Club Bandung HRD Division in processing member data. Created using Microsoft Webmatrix as an editor with the PHP programming language. The database uses MySQL with PHPMyAdmin as the software. The method used in making this software is prototyping so that between developers and customers can understand each other what the customer wants. The purpose of making web-based member data management software is that in terms of managing member data it can be done anytime and anywhere by just accessing the internet. In the use of the program, the author chose to use PHP, because PHP is the best and easiest to use in website programming language. Based on the last paragraph, the author intends to make aplication based computerized attendance so that become effective and efficient in terms of time.

Citation Tools and Reference Management Software for Academic Writing

GIS Business ◽  
2019 ◽  
Vol 14 (6) ◽  
pp. 586-596
Author(s):  
Dr. C. Vijai ◽  
Dr. K. Natarajan ◽  
Mr. M. Elayaraja
Keyword(s):  
Academic Writing ◽  
Journal Article ◽  
Management Software ◽  
Reference Tools

The correct citation of references is an honor to original ideas of previous authors and to avoid plagiarism. Currently, researchers can easily find, cite and store references using citation management software. This paper is discussed how to use popular citation management software (Zotero •Mendeley •EndNote •RefWorks •Citavi •PaperPile •JabRef •Papers •Docear) and reference tools in academic writing. The citation management software is very helpful for academic writing. Exactly what the name suggests: Reference management software used to manage the references in a document and information about a publication,book, journal article, video, etc. This study is based on the available sources from various university online libraries worldwide. This study very uses full for researchers, authors, reviewers, editors and the researcher community.

ARCHITECTURE OF THE REPAIR AND MAINTENANCE MANAGEMENT SOFTWARE FOR A SERVICE ENTERPRISE

2020 ◽  
Vol 2020 (4) ◽  
pp. 53-59
Author(s):  
Dmitriy Titarev ◽  
Andrey Serikov ◽  
Sergei Krivtsanov
Keyword(s):  
Asset Management ◽  
Software Package ◽  
Service Management ◽  
Research Work ◽  
Maintenance Management ◽  
Management Software ◽  
Advantages And Disadvantages

The paper provides an overview of the architectures for the repair and maintenance management software package for a service enterprise. As part of the research work, asset management (EAM) and service management (ITSM) methodologies were studied. Three different architectures for the designed software package are proposed, their descriptions, advantages and disadvantages are given.

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