Amylose-3,5-dimethylphenylcarbamate immobilized on monolithic silica stationary phases for chiral separations in capillary electrochromatography

2012 ◽  
Vol 33 (11) ◽  
pp. 1613-1623 ◽  
Author(s):  
Yueqi Liu ◽  
Yvan Vander Heyden ◽  
Debby Mangelings
Keyword(s):  
Capillary Electrochromatography ◽  
Stationary Phases ◽  
Chiral Separations ◽  
Monolithic Silica

scholarly journals Recent Advances in Chiral Analysis of Proteins and Peptides

Separations ◽  
2021 ◽  
Vol 8 (8) ◽  
pp. 112
Author(s):  
Marine Morvan ◽  
Ivan Mikšík
Keyword(s):  
Amino Acids ◽  
Stationary Phases ◽  
Enzymatic Digestion ◽  
Chiral Separations ◽  
Biological Compounds ◽  
Health And Disease ◽  
Electrophoretic Techniques ◽  
A Site ◽  
Future Work ◽  
Proteins And Peptides

Like many biological compounds, proteins are found primarily in their homochiral form. However, homochirality is not guaranteed throughout life. Determining their chiral proteinogenic sequence is a complex analytical challenge. This is because certain D-amino acids contained in proteins play a role in human health and disease. This is the case, for example, with D-Asp in elastin, β-amyloid and α-crystallin which, respectively, have an action on arteriosclerosis, Alzheimer's disease and cataracts. Sequence-dependent and sequence-independent are the two strategies for detecting the presence and position of D-amino acids in proteins. These methods rely on enzymatic digestion by a site-specific enzyme and acid hydrolysis in a deuterium or tritium environment to limit the natural racemization of amino acids. In this review, chromatographic and electrophoretic techniques, such as LC, SFC, GC and CE, will be recently developed (2018–2020) for the enantioseparation of amino acids and peptides. For future work, the discovery and development of new chiral stationary phases and derivatization reagents could increase the resolution of chiral separations.

Recent progress in adsorbed stationary phases for capillary electrochromatography

2002 ◽  
Vol 23 (22-23) ◽  
pp. 3954-3972 ◽  
Author(s):  
Zhen Liu ◽  
Ren'an Wu ◽  
Hanfa Zou
Keyword(s):  
Capillary Electrochromatography ◽  
Recent Progress ◽  
Stationary Phases
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