Optimization of protein sample preparation for two-dimensional electrophoresis

2012 ◽  
Vol 33 (13) ◽  
pp. 1947-1957 ◽  
Author(s):  
Kristin N. Valente ◽  
Leila H. Choe ◽  
Abraham M. Lenhoff ◽  
Kelvin H. Lee
Keyword(s):  
Sample Preparation ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Protein Sample ◽  
Dimensional Electrophoresis ◽  
Protein Sample Preparation

scholarly journals Standardization of Sample Preparation for Two-Dimensional Electrophoresis of Cryptosporidium parvum Sporozoites

1970 ◽  
Vol 24 (2) ◽  
pp. 119-124
Author(s):  
AMAM Zonaed Siddiki
Keyword(s):  
Sample Preparation ◽  
Proteomic Analysis ◽  
Host Location ◽  
Protein Extraction ◽  
Therapeutic Agents ◽  
Automated Image Analysis ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis ◽  
Important Parasite

Cryptosporidium is an important parasite of human and animals that belongs to the group Apicomplexa. However, the organism diverges from other Apicomplexa in several important aspects such as its unusual host location, atypical developmental biology, unique metabolism and recently described relict mitochondria. All or some of these unique features may be responsible for the lack of effective therapeutic agents against this parasite. The recent completion of genome sequence projects for C. parvum and C. hominis facilitated postgenomic investigations including proteomic analysis. Sample preparation is the first important step for any proteomic analysis. In this study we have attempted to develop the suitable sample preparation protocol for successful two-dimensional electrophoresis (2-DE) of Cryptosporidium sporozoite proteins prior to mass spectrometry. The 2-DE gels were analysed by automated image analysis software and number of protein spots were used as the indicator for maximum protein extraction from Cryptosporidium sporozoite samples. Keywords: Proteomics, Cryptosporidium, Two-dimensional electrophoresis (2-DE), SolubilizationDOI: http://dx.doi.org/10.3329/bjm.v24i2.1255 Bangladesh J Microbiol, Volume 24, Number 2, December 2007, pp 119-124

Identification of human myocardial proteins separated by two-dimensional electrophoresis using an effective sample preparation for mass spectrometry

1996 ◽  
Vol 17 (10) ◽  
pp. 1643-1650 ◽  
Author(s):  
Albrecht Otto ◽  
Bernd Thiede ◽  
Eva-Christina Müller ◽  
Christian Scheler ◽  
Brigitte Wittmann-Liebold ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis
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