Characterization of rhinovirus subviral A particles via capillary electrophoresis, electron microscopy and gas-phase electrophoretic mobility molecular analysis: Part I

2012 ◽  
Vol 33 (12) ◽  
pp. 1833-1841 ◽  
Author(s):  
Victor U. Weiss ◽  
Xavier Subirats ◽  
Angela Pickl-Herk ◽  
Gerhard Bilek ◽  
Wolfgang Winkler ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Capillary Electrophoresis ◽  
Gas Phase ◽  
Electrophoretic Mobility ◽  
Molecular Analysis

Characterization of rhinovirus subviral A particles via capillary electrophoresis, electron microscopy and gas phase electrophoretic mobility molecular analysis: Part II

2013 ◽  
Vol 34 (11) ◽  
pp. 1600-1609 ◽  
Author(s):  
Xavier Subirats ◽  
Victor U. Weiss ◽  
Irene Gösler ◽  
Christoph Puls ◽  
Andreas Limbeck ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Capillary Electrophoresis ◽  
Gas Phase ◽  
Electrophoretic Mobility ◽  
Molecular Analysis

scholarly journals Analysis of a Common Cold Virus and Its Subviral Particles by Gas-Phase Electrophoretic Mobility Molecular Analysis and Native Mass Spectrometry

2015 ◽  
Vol 87 (17) ◽  
pp. 8709-8717 ◽  
Author(s):  
Victor U. Weiss ◽  
Jessica Z. Bereszcazk ◽  
Marlene Havlik ◽  
Peter Kallinger ◽  
Irene Gösler ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Phase ◽  
Electrophoretic Mobility ◽  
Molecular Analysis ◽  
Common Cold ◽  
Native Mass Spectrometry ◽  
Subviral Particles

scholarly journals Separation and Characterization of Highly Charged Polyelectrolytes Using Free-Solution Capillary Electrophoresis

Polymers ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 1331
Author(s):  
Isabelle Desvignes ◽  
Joseph Chamieh ◽  
Hervé Cottet
Keyword(s):  
Capillary Electrophoresis ◽  
Ionic Strength ◽  
Charge Density ◽  
Electrophoretic Mobility ◽  
Separation Mechanism ◽  
Free Solution ◽  
Counterion Condensation ◽  
Charge Densities ◽  
Effective Mobility

The characterization of statistical copolymers of various charge densities remains an important and challenging analytical issue. Indeed, the polyelectrolyte (PE) effective electrophoretic mobility tends to level off above a certain charge density, due to the occurrence of Manning counterion condensation. Surprisingly, we demonstrate in this work that it is possible to get highly resolutive separations of charged PE using free-solution capillary electrophoresis, even above the critical value predicted by the Manning counterion condensation theory. Full separation of nine statistical poly(acrylamide-co-2-acrylamido-2-methylpropanesulfonate) polymers of different charge densities varying between 3% and 100% was obtained by adjusting the ionic strength of the background electrolyte (BGE) in counter electroosmotic mode. Distributions of the chemical charge density could be obtained for the nine PE samples, showing a strong asymmetry of the distribution for the highest-charged PE. This asymmetry can be explained by the different reactivity ratios during the copolymerization. To shed more light on the separation mechanism, effective and apparent selectivities were determined by a systematic study and modeling of the electrophoretic mobility dependence according to the ionic strength. It is demonstrated that the increase in resolution with increasing BGE ionic strength is not only due to a closer matching of the electroosmotic flow magnitude with the PE electrophoretic effective mobility, but also to an increase of the dependence of the PE effective mobility according to the charge density.

scholarly journals Characterization of Diblock Copolymers by Capillary Electrophoresis: From Electrophoretic Mobility Distribution to Distribution of Composition

2019 ◽  
Vol 53 (1) ◽  
pp. 334-345
Author(s):  
Anthony Phimphachanh ◽  
Joseph Chamieh ◽  
Laurent Leclercq ◽  
Simon Harrisson ◽  
Mathias Destarac ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Electrophoretic Mobility ◽  
Diblock Copolymers

scholarly journals Nano electrospray gas-phase electrophoretic mobility molecular analysis (nES GEMMA) of liposomes: applicability of the technique for nano vesicle batch control

The Analyst ◽  
2016 ◽  
Vol 141 (21) ◽  
pp. 6042-6050 ◽  
Author(s):  
Victor U. Weiss ◽  
Carlos Urey ◽  
Andreas Gondikas ◽  
Monika Golesne ◽  
Gernot Friedbacher ◽  
...  
Keyword(s):  
Gas Phase ◽  
Electrophoretic Mobility ◽  
Molecular Analysis ◽  
Charged Particles ◽  
Capacity Determination ◽  
Batch Control

Gas-phase electrophoresis of single-charged particles enables liposome characterization and finally the resulting vesicle encapsulation capacity determination.

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