Enhanced detection of gold nanoparticles in agarose gel electrophoresis

2012 ◽  
Vol 33 (8) ◽  
pp. 1251-1254 ◽  
Author(s):  
Carina Hasenoehrl ◽  
Colleen M. Alexander ◽  
Nicholas N. Azzarelli ◽  
James C. Dabrowiak
Keyword(s):  
Gold Nanoparticles ◽  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis

scholarly journals Connecting DNA Origami Structures into a Designed and Functionalized Network

2019 ◽  
Vol 8 (4) ◽  
pp. 14
Author(s):  
George Amoako
Keyword(s):  
Gold Nanoparticles ◽  
Gel Electrophoresis ◽  
Dna Origami ◽  
Nanometer Scale ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Attachment Sites ◽  
Vis Spectroscopy ◽  
Specific Affinity ◽  
Affinity Interaction

The versatility of the DNA origami approach of organizing nanoparticles at the nanometer scale, together with thiol chemistry have been used. These approaches were used to design DNA origami structures and to functionalize them with gold nanoparticles after designing attachment sites on the DNA origami structures. In all two structures were designed – a cross-like structure and a nanotube but only the nanotube structure was used to form the gold nanoparticle helices. Finally, use was made of the specific affinity interaction between biotin and streptavidin to connect the DNA origami templated AuNP helices to the cross-like structure. Agarose gel electrophoresis, UV-vis spectroscopy and TEM were used to characterize the structure.

Separation of different shape biosynthesized gold nanoparticles via agarose gel electrophoresis

2015 ◽  
Vol 151 ◽  
pp. 332-337 ◽  
Author(s):  
Yanmei Zheng ◽  
Yingling Hong ◽  
Weiwei Wu ◽  
Daohua Sun ◽  
Yuanpeng Wang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis

Luminography – an Alternative Assay for Detection of von Willebrand Factor Multimers

1988 ◽  
Vol 60 (02) ◽  
pp. 133-136 ◽  
Author(s):  
R Schneppenheim ◽  
H Plendl ◽  
U Budde
Keyword(s):  
Von Willebrand Factor ◽  
Gel Electrophoresis ◽  
Detection Methods ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Luminescence Assay ◽  
Von Willebrand ◽  
Willebrand Factor

SummaryA luminescence assay was adapted for detection of von Willebrand factor multimers subsequent to SDS-agarose gel electrophoresis and electroblotting onto nitrocellulose. The method is as fast as chromogenic detection methods and appears to be as sensitive as autoradiography without the disadvantages of the latter.

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