scholarly journals Erratum: High-throughput negative detection of SDS-PAGE separated proteins and its application for proteomics

2010 ◽  
Vol 31 (16) ◽  
pp. 2858-2858
Author(s):  
Wei-Tao Cong ◽  
Sun-Young Hwang ◽  
Li-Tai Jin ◽  
Hong-Zhang He ◽  
Jung-Kap Choi
Keyword(s):  
High Throughput ◽  
Sds Page ◽  
Negative Detection

scholarly journals High-throughput negative detection of SDS-PAGE separated proteins and its application for proteomics

2010 ◽  
Vol 31 (2) ◽  
pp. 411-420 ◽  
Author(s):  
Wei-Tao Cong ◽  
Sun-Young Hwang ◽  
Li-Tai Jin ◽  
Hong-Zhang He ◽  
Jung-Kap Choi
Keyword(s):  
High Throughput ◽  
Sds Page ◽  
Negative Detection

Advanced negative detection method comparable to silver stain for SDS-PAGE separated proteins detection

2016 ◽  
Vol 510 ◽  
pp. 21-25 ◽  
Author(s):  
Xu Wang ◽  
Sun-Young Hwang ◽  
Wei-Tao Cong ◽  
Li-Tai Jin ◽  
Jung-Kap Choi
Keyword(s):  
Detection Method ◽  
Silver Stain ◽  
Sds Page ◽  
Negative Detection

3-D organization of fibrinogen receptors using computer reconstruction and whole-mount microscopy

1988 ◽  
Vol 46 ◽  
pp. 30-31
Author(s):  
E. T. O'Toole ◽  
R. R. Hantgan ◽  
J. C. Lewis
Keyword(s):  
Whole Blood ◽  
Colloidal Gold ◽  
Blood Platelets ◽  
Cell Contact ◽  
Computer Assisted ◽  
Adherent Cells ◽  
Differential Centrifugation ◽  
Computer Reconstruction ◽  
Sds Page ◽  
Whole Mount

Thrombocytes (TC), the avian equivalent of blood platelets, support hemostasis by aggregating at sites of injury. Studies in our lab suggested that fibrinogen (fib) is a requisite cofactor for TC aggregation but operates by an undefined mechanism. To study the interaction of fib with TC and to identify fib receptors on cells, fib was purified from pigeon plasma, conjugated to colloidal gold and used both to facilitate aggregation and as a receptor probe. Described is the application of computer assisted reconstruction and stereo whole mount microscopy to visualize the 3-D organization of fib receptors at sites of cell contact in TC aggregates and on adherent cells.Pigeon TC were obtained from citrated whole blood by differential centrifugation, washed with Ca++ free Hank's balanced salts containing 0.3% EDTA (pH 6.5) and resuspended in Ca++ free Hank's. Pigeon fib was isolated by precipitation with PEG-1000 and the purity assessed by SDS-PAGE. Fib was conjugated to 25nm colloidal gold by vortexing and the conjugates used as the ligand to identify fib receptors.

154: Integration of TPSA and High-Throughput Mass Spectrometry Data Improves Prostate Cancer Prediction

2007 ◽  
Vol 177 (4S) ◽  
pp. 52-53
Author(s):  
Stefano Ongarello ◽  
Eberhard Steiner ◽  
Regina Achleitner ◽  
Isabel Feuerstein ◽  
Birgit Stenzel ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Mass Spectrometry ◽  
High Throughput ◽  
Mass Spectrometry Data ◽  
Cancer Prediction
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