Step width, spacing, and resolution in gradient elution moving boundary electrophoresis. Part 1. Theory and comparison with zone electrophoresis

2010 ◽  
Vol 31 (22) ◽  
pp. 3650-3657 ◽  
Author(s):  
David Ross
Keyword(s):  
Moving Boundary ◽  
Gradient Elution ◽  
Step Width ◽  
Zone Electrophoresis

2D separations on a 1D chip: gradient elution moving boundary electrophoresis—chiral capillary zone electrophoresis

Lab on a Chip ◽  
2010 ◽  
Vol 10 (22) ◽  
pp. 3139 ◽  
Author(s):  
David Ross ◽  
Jonathan G. Shackman ◽  
Jason G. Kralj ◽  
Javier Atencia
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Moving Boundary ◽  
Gradient Elution ◽  
Zone Electrophoresis ◽  
Capillary Zone

Microchip analysis of lithium in blood using moving boundary electrophoresis and zone electrophoresis

2005 ◽  
Vol 26 (15) ◽  
pp. 3032-3042 ◽  
Author(s):  
Elwin X. Vrouwe ◽  
Regina Luttge ◽  
Wouter Olthuis ◽  
Albert van den Berg
Keyword(s):  
Moving Boundary ◽  
Zone Electrophoresis

Expanding the Capabilities of Microfluidic Gradient Elution Moving Boundary Electrophoresis for Complex Samples

2011 ◽  
Vol 83 (16) ◽  
pp. 6316-6322 ◽  
Author(s):  
Elizabeth A. Strychalski ◽  
Alyssa C. Henry ◽  
David Ross
Keyword(s):  
Moving Boundary ◽  
Gradient Elution ◽  
Complex Samples

Sex and sex-cycle differences in rat serum protein concentrations

1959 ◽  
Vol 197 (1) ◽  
pp. 135-137 ◽  
Author(s):  
Werner G. Heim
Keyword(s):  
Moving Boundary ◽  
Serum Proteins ◽  
Female Rats ◽  
Estrus Cycle ◽  
Rat Serum ◽  
Male And Female ◽  
Male And Female Rats ◽  
Blood Serum Proteins ◽  
Zone Electrophoresis ◽  
Sex Cycle

The relative concentrations of the various blood serum proteins, and especially of albumin, were found to be significantly different in male and female rats when examined by moving boundary or zone electrophoresis. However, no significant differences were noted in the sera obtained from rats at different points in the estrus cycle.

THE PROTEOLYTIC ENZYMES OF MICROORGANISMS: VI. THE SEPARATION OF PROTEASES FROM MORTIERELLA RENISPORA DIXON-STEWART BY ZONE ELECTROPHORESIS

1954 ◽  
Vol 32 (1) ◽  
pp. 20-26 ◽  
Author(s):  
L. R. Wetter
Keyword(s):  
Filter Paper ◽  
Active Component ◽  
Culture Medium ◽  
Moving Boundary ◽  
Proteolytic Enzymes ◽  
Potato Starch ◽  
Paper Electrophoresis ◽  
Active Components ◽  
Zone Electrophoresis ◽  
The Difference

Moving-boundary electrophoresis indicated that a protease concentrate obtained from the culture medium of Mortierella renispora Dixon-Stewart (PRL 26) was made up of a number of proteins. Filter-paper electrophoresis demonstrated that two of the proteins were capable of hydrolyzing denatured hemoglobin. One active component had a negative mobility, the other a positive mobility in phosphate buffer pH 6.8, ionic strength 0.1. Because of the difference in electrical properties it was possible to separate the two active components by zone electrophoresis. Though yields were low when filter paper was employed, the use of potato starch as the supporting medium resulted in excellent recoveries.

THE PROTEOLYTIC ENZYMES OF MICROORGANISMS: VI. THE SEPARATION OF PROTEASES FROM MORTIERELLA RENISPORA DIXON-STEWART BY ZONE ELECTROPHORESIS

1954 ◽  
Vol 32 (1) ◽  
pp. 20-26 ◽  
Author(s):  
L. R. Wetter
Keyword(s):  
Filter Paper ◽  
Active Component ◽  
Culture Medium ◽  
Moving Boundary ◽  
Proteolytic Enzymes ◽  
Potato Starch ◽  
Paper Electrophoresis ◽  
Active Components ◽  
Zone Electrophoresis ◽  
The Difference

Moving-boundary electrophoresis indicated that a protease concentrate obtained from the culture medium of Mortierella renispora Dixon-Stewart (PRL 26) was made up of a number of proteins. Filter-paper electrophoresis demonstrated that two of the proteins were capable of hydrolyzing denatured hemoglobin. One active component had a negative mobility, the other a positive mobility in phosphate buffer pH 6.8, ionic strength 0.1. Because of the difference in electrical properties it was possible to separate the two active components by zone electrophoresis. Though yields were low when filter paper was employed, the use of potato starch as the supporting medium resulted in excellent recoveries.

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