Microfluidic droplet trapping array as nanoliter reactors for gas-liquid chemical reaction

2009 ◽  
Vol 30 (18) ◽  
pp. 3181-3188 ◽  
Author(s):  
Qingquan Zhang ◽  
Shaojiang Zeng ◽  
Jianhua Qin ◽  
Bingcheng Lin
Keyword(s):  
Chemical Reaction ◽  
Liquid Chemical ◽  
Droplet Trapping

scholarly journals Errata: Microfluidic droplet trapping array as nanoliter reactors for gas-liquid chemical reaction

2010 ◽  
Vol 31 (8) ◽  
pp. 1431-1431
Author(s):  
Qingquan Zhang ◽  
Shaojiang Zeng ◽  
Jianhua Qin ◽  
Bingcheng Lin
Keyword(s):  
Chemical Reaction ◽  
Liquid Chemical ◽  
Droplet Trapping

Activation of a solid-liquid chemical reaction by ultrasound

1995 ◽  
Vol 50 (3) ◽  
pp. 554-558 ◽  
Author(s):  
Ratoarinoro ◽  
F. Contamine ◽  
A.M. Wilhelm ◽  
J. Berlan ◽  
H. Delmas
Keyword(s):  
Chemical Reaction ◽  
Liquid Chemical ◽  
Solid Liquid

Directional observation on lipid of male nectary of Lindera megaphylla hemsl. by the rapid embedding method with polyethylene glycol (PEG)

1990 ◽  
Vol 48 (3) ◽  
pp. 718-719
Author(s):  
Dai Dalin ◽  
Guo Jianmin
Keyword(s):  
Electron Microscope ◽  
Polyethylene Glycol ◽  
Chemical Reaction ◽  
Traditional Method ◽  
Osmium Tetroxide ◽  
Unsaturated Lipid ◽  
Embedding Method ◽  
Long Period ◽  
New Methods

Lipid cytochemistry has not yet advanced far at the EM level. A major problem has been the loss of lipid during dehydration and embedding. Although the adoption of glutaraldehyde and osmium tetroxide accelerate the chemical reaction of lipid and osmium tetroxide can react on the double bouds of unsaturated lipid to from the osmium black, osmium tetroxide can be reduced in saturated lipid and subsequently some of unsaturated lipid are lost during dehydration. In order to reduce the loss of lipid by traditional method, some researchers adopted a few new methods, such as the change of embedding procedure and the adoption of new embedding media, to solve the problem. In a sense, these new methods are effective. They, however, usually require a long period of preparation. In this paper, we do research on the fiora nectary strucure of lauraceae by the rapid-embedding method wwith PEG under electron microscope and attempt to find a better method to solve the problem mentioned above.

Immunocytochemistry after fast freeze fixation-freeze substitution: Advantages and limits

1990 ◽  
Vol 48 (3) ◽  
pp. 42-43
Author(s):  
Marie-Thérèse Nicolas
Keyword(s):  
Osmium Tetroxide ◽  
Freeze Substitution ◽  
Acrylic Resin ◽  
Expected Improvement ◽  
List Type ◽  
Chemical Fixation ◽  
Freeze Fixation ◽  
Liquid Chemical ◽  
Luciferase Enzyme ◽  
Lower Background

An alternative to aqueous chemical fixation consists in immobilizing physically the specimen by freezing it as fast as possible without using any cryoprotectant. This Fast Freeze Fixation (FFF) followed by Freeze Substitution (FS) avoids osmotic artefacts due to the slow penetration of liquid chemical fixative. Associated with Immuno-Gold labeling (IGS), FFF-FS allows a more precise localization of antigens.Using the bioluminescent bacteria Vibrio harveyi, a comparison of IGS with an antibody directed against its luciferase (enzyme of the luminescent reaction) has been done after liquid chemical fixation versus FFFFS. This later technique, beside an expected improvement of the ultrastructure always shows a better preservation of antigenicity and a lower background. In the case of FFF-FS technique (Figure 3):–labeling in acrylic resin (LRWhite) is 2 to 4 fold more intense than in epoxy resin (Epon),–but the ultrastructure is always better in Epon.–but the ultrastructure is always better in Epon.–The addition of fixatives in the substitution medium, results in a decrease of labeling which is more important in the case of a mixture of fixatives than with osmium tetroxide alone; with one exception: the substitution with glutaraldehyde which produces a dramatic increase in the density of the labeling but also, at the same time, a swelling of the cells of about 30%.

Sign in / Sign up

Export Citation Format

Share Document