A capillary electrophoretic system based on a novel microemulsion for the analysis of coenzyme Q10 in human plasma by electrokinetic chromatography

2009 ◽  
Vol 30 (11) ◽  
pp. 1899-1905 ◽  
Author(s):  
Silvia Lucangioli ◽  
Flor Sabrina ◽  
Contin Mario ◽  
Tripodi Valeria
Keyword(s):  
Human Plasma ◽  
Coenzyme Q10 ◽  
Electrokinetic Chromatography ◽  
Capillary Electrophoretic

Capillary electrophoretic and micellar electrokinetic separations of asymmetric dimethyl-L-arginine and structurally related amino acids: Quantitation in human plasma

2004 ◽  
Vol 27 (17-18) ◽  
pp. 1483-1490 ◽  
Author(s):  
Gabriele Trapp ◽  
Karsten Sydow ◽  
Maria T. Dulay ◽  
Tina Chou ◽  
John P. Cooke ◽  
...  
Keyword(s):  
Amino Acids ◽  
Human Plasma ◽  
Electrokinetic Separations ◽  
Capillary Electrophoretic

scholarly journals HPLC Analysis of Reduced and Oxidized Coenzyme Q10 in Human Plasma

2001 ◽  
Vol 47 (2) ◽  
pp. 256-265 ◽  
Author(s):  
Peter H Tang ◽  
Michael V Miles ◽  
Antonius DeGrauw ◽  
Andrew Hershey ◽  
Amadeo Pesce
Keyword(s):  
Human Plasma ◽  
Coenzyme Q10 ◽  
Venous Blood ◽  
Clinical Importance ◽  
Healthy Individuals ◽  
Reduction Procedure ◽  
Coulometric Detection ◽  
Evacuated Tubes ◽  
Reliable Quantification ◽  
Apparently Healthy

Abstract Background: The percentage of reduced coenzyme Q10 (CoQ10H2) in total coenzyme Q10 (TQ10) is decreased in plasma of patients with prematurity, hyperlipidemia, and liver disease. CoQ10H2 is, however, easily oxidized and difficult to measure, and therefore reliable quantification of plasma CoQ10H2 is of clinical importance. Methods: Venous blood was collected into evacuated tubes containing heparin, which were immediately placed on ice and promptly centrifuged at 4 °C. The plasma was harvested and stored in screw-top polypropylene tubes at −80 °C until analysis. After extraction with 1-propanol and centrifugation, the supernatant was injected directly into an HPLC system with coulometric detection. Results: The in-line reduction procedure permitted transformation of CoQ10 into CoQ10H2 and avoided artifactual oxidation of CoQ10H2. The electrochemical reduction yielded 99% CoQ10H2. Only 100 μL of plasma was required to simultaneously measure CoQ10H2 and CoQ10 over an analytical range of 10 μg/L to 4 mg/L. Intra- and interassay CVs for CoQ10 in human plasma were 1.2–4.9% across this range. Analytical recoveries were 95.8–101.0%. The percentage of CoQ10H2 in TQ10 was ∼96% in apparently healthy individuals. The method allowed analysis of up to 40 samples within an 8-h period. Conclusions: This optimized method for CoQ10H2 analysis provides rapid and precise results with the potential for high throughput. This method is specific and sufficiently sensitive for use in both clinical and research laboratories.

Characterization of antithrombin III from human plasma by two-dimensional gel electrophoresis and capillary electrophoretic methods

2003 ◽  
Vol 24 (24) ◽  
pp. 4282-4290 ◽  
Author(s):  
Leopold Kremser ◽  
Andrea Brückner ◽  
Andrea Heger ◽  
Tom Grunert ◽  
Andrea Buchacher ◽  
...  
Keyword(s):  
Human Plasma ◽  
Gel Electrophoresis ◽  
Antithrombin Iii ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Capillary Electrophoretic

scholarly journals Electrokinetic chromatography for drug analysis. Separation and determination of cefpiramide in human plasma.

1989 ◽  
Vol 37 (3) ◽  
pp. 707-711 ◽  
Author(s):  
Terumichi NAKAGAWA ◽  
Yoshiya ODA ◽  
Akimasa SHIBUKAWA ◽  
Hisako FUKUDA ◽  
Hisashi TANAKA
Keyword(s):  
Human Plasma ◽  
Drug Analysis ◽  
Electrokinetic Chromatography
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