Influence of image-analysis software on quantitation of two-dimensional gel electrophoresis data

2009 ◽  
Vol 30 (2) ◽  
pp. 325-328 ◽  
Author(s):  
Martina Stessl ◽  
Christian R. Noe ◽  
Bodo Lachmann
Keyword(s):  
Image Analysis ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Analysis Software ◽  
Two Dimensional Gel Electrophoresis ◽  
Image Analysis Software

Advances in two-dimensional gel matching technology

2004 ◽  
Vol 32 (3) ◽  
pp. 511-516 ◽  
Author(s):  
S. Church
Keyword(s):  
Nonlinear Dynamics ◽  
Image Analysis ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Analysis Software ◽  
Two Dimensional Gel Electrophoresis ◽  
Software Products ◽  
Large Numbers ◽  
Noise Contamination ◽  
The Impact

For many years, two-dimensional gel electrophoresis has been the method of choice for the investigation of complex mixtures of proteins. Although there are a number of emerging technologies that can be applied to proteomics, none can yet yield routinely the breadth of information available from two-dimensional gels. To be able to obtain instant information regarding molecular mass and pI, as well as to highlight quickly the expression changes or unique proteins across a gel series requires sophisticated and powerful image analysis software. The range of software products offered by Nonlinear Dynamics covers all levels of user application and throughput, from the user-guided Phoretix two-dimensional approach, when working with a small number of gels, to the automatic processing of large numbers of gels with minimal user intervention with Progenesis. Integration of the analysis software with powerful database components allows advanced gel comparisons and data mining to be performed with statistical verification of the results. Spot pick lists can be quickly created and automatically linked to a number of commercially available spot picking robots further increasing the support for proteomics research. The importance of image analysis for accurate, reliable and meaningful results will be discussed. Recent advances in development, with particular attention placed on the impact of noise contamination within gels, are illustrated and how the Progenesis product from Nonlinear Dynamics can be utilized to get the most from two-dimensional gel electrophoresis is shown.

scholarly journals Automated three-dimensional measurements of version, inclination, and subluxation

2019 ◽  
Vol 12 (1) ◽  
pp. 31-37
Author(s):  
Dave R Shukla ◽  
Richard J McLaughlin ◽  
Julia Lee ◽  
Ngoc Tram V Nguyen ◽  
Joaquin Sanchez-Sotelo
Keyword(s):  
Computed Tomography ◽  
Image Analysis ◽  
Three Dimensional ◽  
Automated Image Analysis ◽  
Two Dimensional ◽  
Analysis Software ◽  
Glenoid Version ◽  
Image Analysis Software ◽  
Planning Software ◽  
Dimensional Measurements

Background Preoperative planning software has been developed to measure glenoid version, glenoid inclination, and humeral head subluxation on computed tomography (CT) for shoulder arthroplasty. However, most studies analyzing the effect of glenoid positioning on outcome were done prior to the introduction of planning software. Thus, measurements obtained from the software can only be extrapolated to predict failure provided they are similar to classic measurements. The purpose of this study was to compare measurements obtained using classic manual measuring techniques and measurements generated from automated image analysis software. Methods Ninety-five two-dimensional computed tomography scans of shoulders with primary glenohumeral osteoarthritis were measured for version according to Friedman method, inclination according to Maurer method, and subluxation according to Walch method. DICOM files were loaded into an image analysis software (Blueprint, Wright Medical) and the output was compared with values obtained manually using a paired sample t-test. Results Average manual measurements included 13.8° version, 13.2° inclination, and 56.2% subluxation. Average image analysis software values included 17.4° version (3.5° difference, p < 0.0001), 9.2° inclination (3.9° difference, p < 0.001), and 74.2% for subluxation (18% difference, p < 0.0001). Conclusions Glenoid version and inclination values from the software and manual measurement on two-dimensional computed tomography were relatively similar, within approximately 4°. However, subluxation measurements differed by approximately 20%.

scholarly journals Two-dimensional gel electrophoresis (2D-GE) image analysis based on CellProfiler

Medicine ◽  
2020 ◽  
Vol 99 (49) ◽  
pp. e23373
Author(s):  
Jose Arturo Molina-Mora ◽  
Diana Chinchilla-Montero ◽  
Carolina Castro-Peña ◽  
Fernando García
Keyword(s):  
Image Analysis ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis
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