Use of quasi-isoelectric buffers to limit protein adsorption in capillary zone electrophoresis

2008 ◽  
Vol 29 (15) ◽  
pp. 3164-3167 ◽  
Author(s):  
Martine Poitevin ◽  
Karim Hammad ◽  
Ichraf Ayed ◽  
Pier Giorgio Righetti ◽  
Gabriel Peltre ◽  
...  
Keyword(s):  
Protein Adsorption ◽  
Capillary Zone Electrophoresis ◽  
Zone Electrophoresis ◽  
Capillary Zone

scholarly journals PREVENTION OF PROTEIN ADSORPTION ON BARE FUSED-SILICA CAPILLARY BY PEG IN CAPILLARY ZONE ELECTROPHORESIS

2010 ◽  
Vol 9 (3) ◽  
pp. 410-413
Author(s):  
Adhitasari Suratman ◽  
Hermann Waetzig
Keyword(s):  
Protein Adsorption ◽  
Capillary Zone Electrophoresis ◽  
Protein Separation ◽  
Fused Silica ◽  
Buffer Solution ◽  
Relative Standard ◽  
Zone Electrophoresis ◽  
Fused Silica Capillary ◽  
Capillary Zone ◽  
Silica Capillary

The protein separation was studied in capillary zone electrophoresis for preventing protein adsorption on the capillary wall. ß-lactoglobulin (pI: 4.83-5.4, Mr: 18.4 kDa), cytochrome c (pI: 9.59, Mr: 11.7 kDa) and ß-casein (pI: 4.6, Mr: 24 kDa) were used as protein models. Strong adsorption of the proteins occurred onto the capillary at a pH around their pIs. In order to prevent protein adsorption, PEG (Poly(ethylene glycol)) was investigated as an effective substance to stabilize the proteins native state and coat the bare fused-silica capillary surface. The presence of 32 mg/mL PEG in buffer solution in a pH range of 6.0 to 4.0 was successful to suppress protein adsorption during the separation. It can also be confirmed with the reproducibility of apparent EOF mobility with percentile RSD (Relative Standard Deviation) less than 2% in long-term measurement.   Keywords: PEG, protein adsorption, CZE

Zoledronate and Related Impurities Analysis by Capillary Zone Electrophoresis

2014 ◽  
Vol 10 (2) ◽  
pp. 231-234 ◽  
Author(s):  
Gisela Alvarez ◽  
María Foglia ◽  
Daniela Camporotondi ◽  
S. Giorgieri ◽  
Martín Desimone ◽  
...  
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Related Impurities ◽  
Zone Electrophoresis ◽  
Capillary Zone
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