Chiral separation with gradient elution isotachophoresis for futurein situextraterrestrial analysis

2008 ◽  
Vol 29 (19) ◽  
pp. 4036-4044 ◽  
Author(s):  
Grégoire Danger ◽  
David Ross
Keyword(s):  
Chiral Separation ◽  
Gradient Elution ◽  
Gradient Elution Isotachophoresis

DNA purification from crude samples for human identification using gradient elution isotachophoresis

2013 ◽  
Vol 34 (17) ◽  
pp. 2522-2530 ◽  
Author(s):  
Elizabeth A. Strychalski ◽  
Christopher Konek ◽  
Erica L. R. Butts ◽  
Peter M. Vallone ◽  
Alyssa C. Henry ◽  
...  
Keyword(s):  
Human Identification ◽  
Gradient Elution ◽  
Dna Purification ◽  
Gradient Elution Isotachophoresis

Amino Acid Measurements from a High Conductivity Matrix by Gradient Elution Isotachophoresis

2009 ◽  
Vol 70 (1-2) ◽  
pp. 151-156 ◽  
Author(s):  
Chandni A. Vyas ◽  
Manasa Mamunooru ◽  
Jonathan G. Shackman
Keyword(s):  
Amino Acid ◽  
Gradient Elution ◽  
High Conductivity ◽  
Gradient Elution Isotachophoresis ◽  
Conductivity Matrix

Gradient Elution Isotachophoresis for Enrichment and Separation of Biomolecules

2007 ◽  
Vol 79 (17) ◽  
pp. 6641-6649 ◽  
Author(s):  
Jonathan G. Shackman ◽  
David Ross
Keyword(s):  
Gradient Elution ◽  
Gradient Elution Isotachophoresis

Gradient elution isotachophoresis with direct ultraviolet absorption detection for sensitive amino acid analysis

2008 ◽  
Vol 1202 (2) ◽  
pp. 203-211 ◽  
Author(s):  
Manasa Mamunooru ◽  
Ronald J. Jenkins ◽  
Nejea I. Davis ◽  
Jonathan G. Shackman
Keyword(s):  
Amino Acid ◽  
Amino Acid Analysis ◽  
Gradient Elution ◽  
Ultraviolet Absorption ◽  
Gradient Elution Isotachophoresis

Development and Validation of HPLC/UV-Spectrophotometric Procedures for Metronidazole Quantitative Determination

2018 ◽  
Vol 9 (3) ◽  
Author(s):  
Klimenko Lina Yu ◽  
Shkarlat Galyna L ◽  
Shovkova Zoia V ◽  
Yaremenko Vitaliy D ◽  
Shpychak Oleg S
Keyword(s):  
Quantitative Determination ◽  
Sodium Hydroxide Solution ◽  
Gradient Elution ◽  
Calibration Model ◽  
Column Temperature ◽  
Medical Laboratories ◽  
Accuracy And Precision ◽  
Development And Validation ◽  
Gradient Elution Mode ◽  
Potential Object

Metronidazole is the most popular representative of antiprotozoal medicines from the group of 5-nitroimidazoles. Metronidazole blocks the enzymes of alcohol dehydrogenase and acetaldehyde dehydrogenase, therefore when its joint taking with alcohol it is observed the strong intoxication syndrome and fatal poisonings too. Therefore metronidazole can be a potential object of chemical toxicological investigations. The purpose of our paper is to develop HPLC/UV-procedure of metronidazole quantification with application of the system of HPLC-analyzer MiLiChrome® A-0230 implemented in practice of forensic medical laboratories in Russia and Ukraine and carry out step-by-step validation of the developed procedure. Chromatographic conditions: Eluent A (0.2 M LiClO4 – 0.005 M HClO4) and Eluent B (acetonitrile) wereused as the mobile phase components; HPLC microcolumn Ø2×75 mm and ProntoSIL 120-5-C18 AQ, 5 μm were used as an analytical column; temperature was 40°С; flow rate was 100 μl/min; gradient elution mode was from 5% to 100% Eluent B for 40 min, then 100% Eluent B for 3 min; detection was performed at 277 nm. Retention time for metronidazole is 5.95 min. Since metronidazole is easy soluble and stable enough in the solutions of diluted alkalis 0.001 M sodium hydroxide solution has been proposed for preparation of the solutions in developing HPLC/UV-procedure of metronidazole quantification. Validation of the procedure has been carried out in the variants of the method of calibration curve and method of standard by such parameters as in process stability, linearity/calibration model, accuracy and precision within 3 different analytical runs using different batches of reagents and different glassware; experiments have been performed by three different analysts. New procedure of metronidazole quantitative determination by the method of HPLC/UV has been developed. Its validation has been carried out and acceptability for application has been shown.

Reversed-Phase High Performance Liquid Chromatographic Analysis of Three First Line Anti-Tuberculosis Drugs

2019 ◽  
Vol 69 (12) ◽  
pp. 3590-3592
Author(s):  
Nela Bibire ◽  
Romeo Iulian Olariu ◽  
Luminita Agoroaei ◽  
Madalina Vieriu ◽  
Alina Diana Panainte ◽  
...  
Keyword(s):  
High Performance ◽  
Biological Fluids ◽  
Gradient Elution ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Assay Method ◽  
Active Pharmaceutical Ingredients ◽  
First Line ◽  
Pharmaceutical Ingredients ◽  
Liquid Chromatographic

Active pharmaceutical ingredients such as isoniazid, pyrazinamide and rifampicin are among the most important first-line anti-tuberculosis drugs. A simple, rapid and sensitive reversed phase-high performance liquid chromatographic assay method for the simultaneous determination of isoniazid, pyrazinamide and rifampicin has been developed. Separation of the interest compounds was achieved in a 10 min chromatographic run in gradient elution mode on a Zorbax SB-C18 stainless steel column (150 � 4 mm, 5 mm) using a guard column containing the same stationary phase. The gradient elution was carried out with a mobile phase of 10% CH3CN aqueous solution for channel A and 50% CH3CN in pH = 6.8 phosphate buffer (20 mM), to which 1.5 mL triethylamine were added for channel B. Quantification of the analyzed substances was carried out spectrophotometrically at 269 nm. Detection limits of 0.48 mg/L for isoniazid, 0.52 mg/L for pyrazinamide and 0.48 mg/L for rifampicin were established for the developed assay method. The present work showed that the proposed analysis method was advantageous for simple and rapid analysis of the active pharmaceutical ingredients in pharmaceuticals and biological fluids.

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