Investigation of hormone activity in butterfly imaginal wing discs by protein expression pattern changes

2007 ◽  
Vol 28 (4) ◽  
pp. 535-544 ◽  
Author(s):  
Alexandra Cieslak ◽  
Alfried P. Vogler ◽  
Nathalie Lafranchi-Tristem ◽  
Michael J. Dunn ◽  
Jules A. Westbrook
Keyword(s):  
Protein Expression ◽  
Expression Pattern ◽  
Protein Expression Pattern ◽  
Hormone Activity ◽  
Wing Discs

scholarly journals Doublesexmediates the development of sex-specific pheromone organs inBicyclusbutterflies via multiple mechanisms

2019 ◽  
Author(s):  
Anupama Prakash ◽  
Antónia Monteiro
Keyword(s):  
Protein Expression ◽  
Expression Pattern ◽  
Chemical Communication ◽  
Molecular Basis ◽  
Organ Development ◽  
Protein Expression Pattern ◽  
Bicyclus Anynana ◽  
Spatial Regulation ◽  
Wing Discs ◽  
Male Specific

AbstractTheBicycluslineage of satyrid butterflies exhibits male-specific traits, the scent organ complex, used for chemical communication during courtship. This complex consists of tightly packed brush-like scales (hair-pencils) that rub against scent patches to disperse pheromones, but the evolution and molecular basis of the organ’s male-limited development remains unknown. Here, we examine the evolution of the number and location of the scent patches and hair-pencils within 53 species ofBicyclusbutterflies, and the involvement of the sex determinant genedoublesex (dsx)in scent organ development inBicyclus anynanausing CRISPR/Cas9. We show that scent patches and hair-pencils arose via multiple, independent gains, in a correlated manner. Further, an initially non-sex-specific Dsx protein expression pattern in developing wing discs becomes male-specific and spatially refined to areas that develop the scent organ complex over the course of development. Functional perturbations ofdsxshow that this gene is required for male patch development whereas hair-pencils can develop in both sexes without Dsx input. Dsx in females is, instead, required to repress hair-pencils. These findings suggest that the patches and hair-pencils evolve as correlated composite organs that are sex-limited via the spatial regulation ofdsx. Divergence in the function ofdsxisoforms occurs in both sexes, where the male isoform promotes patch development in males and the female isoform represses hair-pencil development in females, both leading to the development of male-limited traits. Furthermore, evolution in number and location of patches, but not of hair-pencils, appears to be regulated by spatial regulation ofdsx.

scholarly journals Doublesex Mediates the Development of Sex-Specific Pheromone Organs in Bicyclus Butterflies via Multiple Mechanisms

2020 ◽  
Vol 37 (6) ◽  
pp. 1694-1707 ◽  
Author(s):  
Anupama Prakash ◽  
Antónia Monteiro
Keyword(s):  
Protein Expression ◽  
Expression Pattern ◽  
Chemical Communication ◽  
Molecular Basis ◽  
Functional Integration ◽  
Protein Expression Pattern ◽  
Bicyclus Anynana ◽  
Spatial Regulation ◽  
Wing Discs ◽  
Male Specific

Abstract The Bicyclus lineage of satyrid butterflies exhibits male-specific traits, the scent organs, used for chemical communication during courtship. These organs consist of tightly packed brush-like scales (hair-pencils) that rub against scent patches to disperse pheromones, but the evolution and molecular basis of these organ’s male-limited development remains unknown. Here, we examine the evolution of the number and location of the scent patches and hair-pencils within 53 species of Bicyclus butterflies, and the involvement of the sex determinant gene doublesex (dsx) in scent organ development in Bicyclus anynana using CRISPR/Cas9. We show that scent patches and hair-pencils arose via multiple, independent gains, in a correlated manner. Further, an initially nonsex-specific Dsx protein expression pattern in developing wing discs becomes male-specific and spatially refined to areas that develop the scent patches. Functional perturbations of dsx show that this gene activates patch development in males whereas hair-pencils develop in both sexes without Dsx input. Dsx in females is, instead, required to repress hair-pencils whereas Dsx in males regulates minor aspects of its development. These findings suggest that the patches and hair-pencils evolve as correlated composite organs presumably due to their functional integration. Divergence in the function of dsx isoforms occurred across the sexes, where the male isoform promotes patch development in males and the female isoform represses hair-pencil development in females, both leading to the development of male-limited traits. Furthermore, evolution in number of patches in males is due to the evolution of spatial regulation of dsx.

Protein expression pattern of CDK11p58 during testicular development in the mouse

2005 ◽  
Vol 270 (1-2) ◽  
pp. 99-106 ◽  
Author(s):  
Ziyue Niu ◽  
Aiguo Shen ◽  
Hailian Shen ◽  
Jianhai Jiang ◽  
Hongliang Zong ◽  
...  
Keyword(s):  
Protein Expression ◽  
Expression Pattern ◽  
Testicular Development ◽  
Protein Expression Pattern

scholarly journals Matrix Gla Protein expression pattern in the early avian embryo

2016 ◽  
Vol 60 (1-2-3) ◽  
pp. 71-76 ◽  
Author(s):  
Elizabeth Correia ◽  
Natércia Conceição ◽  
M. Leonor Cancela ◽  
José A. Belo
Keyword(s):  
Protein Expression ◽  
Expression Pattern ◽  
Matrix Gla Protein ◽  
Avian Embryo ◽  
Protein Expression Pattern

Protein expression pattern of PAWP in bull spermatozoa is associated with sperm quality and fertility following artificial insemination

2014 ◽  
Vol 81 (5) ◽  
pp. 436-449 ◽  
Author(s):  
Chelsey E. Kennedy ◽  
Kari Beth Krieger ◽  
Miriam Sutovsky ◽  
Wei Xu ◽  
Peter Vargovič ◽  
...  
Keyword(s):  
Protein Expression ◽  
Expression Pattern ◽  
Artificial Insemination ◽  
Sperm Quality ◽  
Protein Expression Pattern

Protein expression pattern in experimental pneumococcal meningitis

2006 ◽  
Vol 8 (4) ◽  
pp. 974-983 ◽  
Author(s):  
Matthias Klein ◽  
Robert Paul ◽  
Barbara Angele ◽  
Bernadette Popp ◽  
Hans-Walter Pfister ◽  
...  
Keyword(s):  
Protein Expression ◽  
Expression Pattern ◽  
Pneumococcal Meningitis ◽  
Protein Expression Pattern ◽  
Experimental Pneumococcal Meningitis

Protein expression pattern distinguishes different lymphoid neoplasms

PROTEOMICS ◽  
2005 ◽  
Vol 5 (16) ◽  
pp. 4274-4286 ◽  
Author(s):  
Kazuyasu Fujii ◽  
Tadashi Kondo ◽  
Hideki Yokoo ◽  
Tesshi Yamada ◽  
Yoshihiro Matsuno ◽  
...  
Keyword(s):  
Protein Expression ◽  
Expression Pattern ◽  
Protein Expression Pattern ◽  
Lymphoid Neoplasms
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