Analysis of choline and atropine in hairy root cultures ofCannabis sativa L. by capillary electrophoresis-electrospray mass spectrometry

2006 ◽  
Vol 27 (11) ◽  
pp. 2208-2215 ◽  
Author(s):  
Imane Wahby ◽  
David Arráez-Román ◽  
Antonio Segura-Carretero ◽  
Francisco Ligero ◽  
Juan Manuel Caba ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Capillary Electrophoresis ◽  
Hairy Root ◽  
Electrospray Mass Spectrometry ◽  
Hairy Root Cultures ◽  
Root Cultures

Alkaloid Spectrum in Diploid and Tetraploid Hairy Root Cultures of Datura stramonium

2003 ◽  
Vol 58 (1-2) ◽  
pp. 42-46 ◽  
Author(s):  
Strahil Berkov ◽  
Atanas Pavlov ◽  
Petia Kovatcheva ◽  
Pepa Stanimirova ◽  
Stefan Philipov
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Hairy Root ◽  
Hairy Roots ◽  
Datura Stramonium ◽  
Gas Chromatography Mass Spectrometry ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Mass Spectral Data ◽  
Mass Spectral

Hairy root cultures were obtained from diploid and induced tetraploid plants of Datura stramonium and analyzed by gas chromatography/mass spectrometry. Twenty alkaloids (19 for diploid and 9 for tetraploid hairy root cultures) were identified. A new tropane ester 3-tigloyloxy-6-propionyloxy-7-hydroxytropane was identified on the basis of mass spectral data. Hyoscyamine was the main alkaloid in both diploid and tetraploid cultures. In contrast to diploid hairy roots, the percentage contributions of the alkaloids, with exceptions for hyoscyamine and apoatropine, were higher in the total alkaloid mixture of tetraploid hairy roots

scholarly journals Variability of Bioactive Glucosinolates, Isothiocyanates and Enzyme Patterns in Horseradish Hairy Root Cultures Initiated from Different Organs

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2828 ◽  
Author(s):  
Bertóti ◽  
Böszörményi ◽  
Alberti ◽  
Béni ◽  
M-Hamvas ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Horseradish Peroxidase ◽  
Hairy Root ◽  
Growth Index ◽  
Biologically Active ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Daily Growth ◽  
Hairy Root Clones ◽  
Enzyme Patterns

Horseradish hairy root cultures are suitable plant tissue organs to study the glucosinolate–myrosinase–isothiocyanate system and also to produce the biologically active isothiocyanates and horseradish peroxidase, widely used in molecular biology. Fifty hairy root clones were isolated after Agrobacterium rhizogenes infection of surface sterilized Armoracia rusticana petioles and leaf blades, from which 21 were viable after antibiotic treatment. Biomass properties (e.g. dry weight %, daily growth index), glucosinolate content (analyzed by liquid chromatography-electronspray ionization-mass spectrometry (LC-ESI-MS/MS)), isothiocyanate and nitrile content (analyzed by gas chromatography-mass spectrometry (GC-MS)), myrosinase (on-gel detection) and horseradish peroxidase enzyme patterns (on-gel detection and spectrophotometry), and morphological features were examined with multi-variable statistical analysis. In addition to the several positive and negative correlations, the most outstanding phenomenon was many parameters of the hairy root clones showed dependence on the organ of origin. Among others, the daily growth index, sinigrin, glucobrassicin, 3-phenylpropionitrile, indole-3-acetonitrile and horseradish peroxidase values showed significantly higher levels in horseradish hairy root cultures initiated from leaf blades.

Elicitor-enhanced Silymarin production in hairy root cultures of Silybum marianum L. Gaertn

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
T Hasanloo ◽  
H Rahnama ◽  
R Sepehrifar ◽  
MR Shams
Keyword(s):  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Silybum Marianum

Clone selection of in vitro horseradish hairy root cultures

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
R Bertóti ◽  
Á Alberti ◽  
A Böszörményi ◽  
R Könye ◽  
T Horváth ◽  
...  
Keyword(s):  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Clone Selection ◽  
Selection Of

scholarly journals Carrot hairy roots: factories for secondary metabolite production

2020 ◽  
Vol 71 (22) ◽  
pp. 6861-6864
Author(s):  
María A Pedreño ◽  
Lorena Almagro
Keyword(s):  
Phenolic Compounds ◽  
Secondary Metabolite ◽  
Hairy Root ◽  
Hairy Roots ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Black Carrot ◽  
Metabolite Production ◽  
Experimental Botany ◽  
Induced Changes

This article comments on: Barba-Espín G, Chen S-T, Agnolet S, Hegelund JN, Stanstrup J, Christensen JH, Müller R, Lütken H. 2020. Ethephon-induced changes in antioxidants and phenolic compounds in anthocyanin-producing black carrot hairy root cultures. Journal of Experimental Botany 71, 7030–7045.

Hairy root cultures of Cynara cardunculus L. as a valuable source of hydroxycinnamic acid compounds

2021 ◽  
Author(s):  
André Folgado ◽  
Ana Teresa Serra ◽  
Inês Prazeres ◽  
Andreia Bento-Silva ◽  
M. Rosário Bronze ◽  
...  
Keyword(s):  
Hairy Root ◽  
Hydroxycinnamic Acid ◽  
Hairy Root Cultures ◽  
Cynara Cardunculus ◽  
Root Cultures ◽  
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