A proteomic approach to investigate potential biomarkers directed against membrane-associated breast cancer proteins

2006 ◽  
Vol 27 (8) ◽  
pp. 1609-1616 ◽  
Author(s):  
Ludovic Canelle ◽  
Jordane Bousquet ◽  
Cedric Pionneau ◽  
Julie Hardouin ◽  
Genevieve Choquet-Kastylevsky ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Proteomic Approach ◽  
Potential Biomarkers

Identification of differentially expressed plasma lncRNAs as potential biomarkers for breast cancer

2021 ◽  
Author(s):  
Minghui Wang ◽  
Huilin Liu ◽  
Wenyao Wu ◽  
Jinxia Zhao ◽  
Guanghui Song ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Differentially Expressed ◽  
Potential Biomarkers

scholarly journals Proteotranscriptomic Profiling of 231-BR Breast Cancer Cells: Identification of Potential Biomarkers and Therapeutic Targets for Brain Metastasis

2015 ◽  
Vol 14 (9) ◽  
pp. 2316-2330 ◽  
Author(s):  
Matthew D. Dun ◽  
Robert J. Chalkley ◽  
Sam Faulkner ◽  
Sheridan Keene ◽  
Kelly A. Avery-Kiejda ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Brain Metastasis ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Therapeutic Targets ◽  
Potential Biomarkers

scholarly journals Whole blood microRNAs as potential biomarkers in post-operative early breast cancer patients

BMC Cancer ◽  
2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Marianna Alunni-Fabbroni ◽  
◽  
Leonie Majunke ◽  
Elisabeth K. Trapp ◽  
Marie Tzschaschel ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Early Breast Cancer ◽  
Whole Blood ◽  
Breast Cancer Patients ◽  
Potential Biomarkers

scholarly journals The Differential Expression of Aqueous Soluble Proteins in Breast Normal and Cancerous Tissues in Relation to Ethnicity of the Patients; Chinese, Malay and Indian

2010 ◽  
Vol 28 (3) ◽  
pp. 149-165 ◽  
Author(s):  
Seng Liang ◽  
Manjit Singh ◽  
Lay-Harn Gam
Keyword(s):  
Breast Cancer ◽  
Ductal Carcinoma ◽  
Protein Profile ◽  
Female Breast Cancer ◽  
Female Mortality ◽  
Normal Tissues ◽  
Infiltrating Ductal Carcinoma ◽  
Female Breast ◽  
Proteomic Approach ◽  
Breast Tissues

Female breast cancer is one of the leading causes of female mortality worldwide. In Malaysia, breast cancer is the most commonly diagnosed cancer in women. Of the women in Malaysia, the Chinese have the highest number of breast cancer cases, followed by the Indian and the Malay. The most common type of breast cancer is infiltrating ductal carcinoma (IDC). A proteomic approach was applied in this study to identify changes in the protein profile of cancerous tissues compared with normal tissues from 18 patients; 8 Chinese, 6 Malay and 4 Indian were analysed. Twenty-four differentially expressed hydrophilic proteins were identified. We evaluated the potential of these proteins as biomarkers for infiltrating ductal carcinoma based on their ethnic-specific expressions. Three of the upregulated proteins, calreticulin, 14-3-3 protein zeta and 14-3-3 protein eta, were found to be expressed at a significantly higher level in the cancerous breast tissues when compared with the normal tissues in cases of infiltrating ductal carcinoma. The upregulation in expression was particularly dominant in the Malay cohort.

scholarly journals Sulforaphane: Expected to Become a Novel Antitumor Compound

2020 ◽  
Vol 28 (4) ◽  
pp. 439-446 ◽  
Author(s):  
Geting Wu ◽  
Yuanliang Yan ◽  
Yangying Zhou ◽  
Yumei Duan ◽  
Shuangshuang Zeng ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Antitumor Effect ◽  
Malignant Tumors ◽  
Inhibitory Effects ◽  
Traditional Chinese Herbal Medicine ◽  
Chinese Herbal ◽  
Alternative Systems ◽  
Antitumor Compound ◽  
Functional Mechanisms ◽  
Potential Biomarkers

Natural products are becoming increasingly popular in a variety of traditional, complementary, and alternative systems due to their potency and slight side effects. Natural compounds have been shown to be effective against many human diseases, especially cancers. Sulforaphane (SFE) is a traditional Chinese herbal medicine. In recent years, an increasing number of studies have been conducted to evaluate the antitumor effect of SFE. The roles of SFE in cancers are mainly through the regulation of potential biomarkers to activate or inhibit related signaling pathways. SFE has exhibited promising inhibitory effects on breast cancer, lung cancer, liver cancer, and other malignant tumors. In this review, we summarized the reports on the activity and functional mechanisms of SFE in cancer treatment and explored the efficacy and toxicity of SFE.

scholarly journals DJ-1 Proteoforms in Breast Cancer Cells: The Escape of Metabolic Epigenetic Misregulation

Cells ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1968
Author(s):  
Domenica Scumaci ◽  
Erika Olivo ◽  
Claudia Vincenza Fiumara ◽  
Marina La Chimia ◽  
Maria Teresa De Angelis ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Oxidative Stress ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Glycolytic Flux ◽  
Proliferative Potential ◽  
Moderate Increase ◽  
Reactive Carbonyl Species ◽  
Proteomic Approach ◽  
Age Related

Enhanced glycolysis is a hallmark of breast cancer. In cancer cells, the high glycolytic flux induces carbonyl stress, a damaging condition in which the increase of reactive carbonyl species makes DNA, proteins, and lipids more susceptible to glycation. Together with glucose, methylglyoxal (MGO), a byproduct of glycolysis, is considered the main glycating agent. MGO is highly diffusible, enters the nucleus, and can react with easily accessible lysine- and arginine-rich tails of histones. Glycation adducts on histones undergo oxidization and further rearrange to form stable species known as advanced glycation end-products (AGEs). This modification alters nucleosomes stability and chromatin architecture deconstructing the histone code. Formation of AGEs has been associated with cancer, diabetes, and several age-related diseases. Recently, DJ-1, a cancer-associated protein that protects cells from oxidative stress, has been described as a deglycase enzyme. Although its role in cell survival results still controversial, in several human tumors, its expression, localization, oxidation, and phosphorylation were found altered. This work aimed to explore the molecular mechanism that triggers the peculiar cellular compartmentalization and the specific post-translational modifications (PTM) that, occurring in breast cancer cells, influences the DJ-1 dual role. Using a proteomic approach, we identified on DJ-1 a novel threonine phosphorylation (T125) that was found, by the in-silico tool scansite 4, as part of a putative Akt consensus. Notably, this threonine is in addition to histidine 126, a key residue involved in the formation of catalytic triade (glu18-Cys106-His126) inside the glioxalase active site of DJ. Interestingly, we found that pharmacological modulation of Akt pathway induces a functional tuning of DJ-1 proteoforms, as well as their shuttle from cytosol to nucleus, pointing out that pathway as critical in the development of DJ-1 pro-tumorigenic abilities. Deglycase activity of DJ-1 on histones proteins, investigated by coupling 2D tau gel with LC-MS/MS and 2D-TAU (Triton-Acid-Urea)-Western blot, was found correlated with its phosphorylation status that, in turn, depends from Akt activation. In normal conditions, DJ-1 acts as a redox-sensitive chaperone and as an oxidative stress sensor. In cancer cells, glycolytic rewiring, inducing increased reactive oxygen species (ROS) levels, enhances AGEs products. Alongside, the moderate increase of ROS enhances Akt signaling that induces DJ-1-phosphorylation. When phosphorylated DJ-1 increases its glyoxalase activity, the level of AGEs on histones decreases. Therefore, phospho-DJ-1 prevents glycation-induced histones misregulation and its Akt-related hyperactivity represents a way to preserve the epigenome landscape sustaining proliferation of cancer cells. Together, these results shed light on an interesting mechanism that cancer cells might execute to escape the metabolic induced epigenetic misregulation that otherwise could impair their malignant proliferative potential.

Identification of co-expression modules and potential biomarkers of breast cancer by WGCNA

Gene ◽  
2020 ◽  
Vol 750 ◽  
pp. 144757 ◽  
Author(s):  
Ruikang Jia ◽  
Huaxu Zhao ◽  
Mengwen Jia
Keyword(s):  
Breast Cancer ◽  
Potential Biomarkers
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