Highly sensitive multistage mass spectrometry enables small-scale analysis of protein glycosylation from two-dimensional polyacrylamide gels

2006 ◽  
Vol 27 (7) ◽  
pp. 1394-1406 ◽  
Author(s):  
Nobuaki Takemori ◽  
Naoka Komori ◽  
Hiroyuki Matsumoto
Keyword(s):  
Mass Spectrometry ◽  
Protein Glycosylation ◽  
Small Scale ◽  
Two Dimensional ◽  
Scale Analysis ◽  
Polyacrylamide Gels ◽  
Highly Sensitive

Identification of Trichloroethanol Visualized Proteins from Two-Dimensional Polyacrylamide Gels by Mass Spectrometry

2006 ◽  
Vol 78 (7) ◽  
pp. 2388-2396 ◽  
Author(s):  
Carol L. Ladner ◽  
Robert A. Edwards ◽  
David C. Schriemer ◽  
Raymond J. Turner
Keyword(s):  
Mass Spectrometry ◽  
Two Dimensional ◽  
Polyacrylamide Gels

Highly sensitive infrared detector based on a two-dimensional heterojunction

2021 ◽  
Vol 14 (1) ◽  
pp. 1-13
Author(s):  
ZHANG Jin-yue ◽  
◽  
LU Jun-peng ◽  
NI Zhen-hua
Keyword(s):  
Infrared Detector ◽  
Two Dimensional ◽  
Highly Sensitive

A Novel Simultaneous Estimation of Sofosbuvir and Velpatasvir in Human Plasma by Liquid Chromatography Tandem-Mass Spectrometry after Protein Precipitation Method

2019 ◽  
Vol 15 (7) ◽  
pp. 710-715
Author(s):  
S.T. Narenderan ◽  
Basuvan Babu ◽  
T. Gokul ◽  
Subramania Nainar Meyyanathan
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Human Plasma ◽  
Multiple Reaction Monitoring ◽  
Simultaneous Estimation ◽  
Pharmacokinetic Studies ◽  
Tandem Mass ◽  
Mass Spectrometric Method ◽  
Highly Sensitive ◽  
Tandem Mass Spectrometric

Objective: The aim of the present work is to achieve a novel highly sensitive chromatographic method for the simultaneous determination of hepatitis C agents, sofosbuvir and velpatasvir from human plasma using ritonavir as an internal standard. Methods: Chromatographic separation was achieved using Hypersil C18 column (50mm x 4.6mm, 3μm) with an isocratic elution mode using the mobile phase composition 10 mM ammonium formate buffer (pH 5.0): acetonitrile (20:80 v/v) pumped at a flow rate of 0.5 ml/min. The detection was carried out by tandem mass spectrometry using Multiple Reaction Monitoring (MRM) positive Electrospray Ionization (ESI) with proton adducts at m/z 530.10 > 243.10, 883.40 > 114.0 and 721.25 > 197.0. Results: The method validated as per USFDA guidelines with respect to linearity, accuracy, and precision was found to be acceptable over the concentration range of 0.2–2000 ng/ml and 5-2000 ng/ml for sofosbuvir and velpatasvir respectively and the method was found to be highly sensitive and selective. Conclusion: The developed tandem mass spectrometric method is robust and can be applied for the monitoring of plasma levels of the analyzed drug in preclinical and clinical pharmacokinetic studies.

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