Effect of background electrolyte on the estimation of protein hydrodynamic radius and net charge through capillary zone electrophoresis

2005 ◽  
Vol 26 (17) ◽  
pp. 3232-3246 ◽  
Author(s):  
Maria V. Piaggio ◽  
Marta B. Peirotti ◽  
Julio A. Deiber
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Hydrodynamic Radius ◽  
Background Electrolyte ◽  
Net Charge ◽  
Zone Electrophoresis ◽  
Capillary Zone

Rapid optimized separation of bromide in serum samples with capillary zone electrophoresis by using glycerol as additive to the background electrolyte

2009 ◽  
Vol 1216 (15) ◽  
pp. 3349-3352 ◽  
Author(s):  
Jennifer P. Pascali ◽  
Eloisa Liotta ◽  
Rossella Gottardo ◽  
Federica Bortolotti ◽  
Franco Tagliaro
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Background Electrolyte ◽  
Serum Samples ◽  
Zone Electrophoresis ◽  
Capillary Zone

scholarly journals Green, Cost-Effective Simultaneous Assay of Chloramphenicol, Methylparaben, and Propylparaben in Eye-Drops by Capillary Zone Electrophoresis

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Thi Thanh Vuong Tong ◽  
Thi Thoa Cao ◽  
Nguyen Ha Tran ◽  
Thi Kim Van Le ◽  
Dinh Chi Le
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Background Electrolyte ◽  
Cost Effective ◽  
Complete Separation ◽  
Sodium Tetraborate ◽  
Eye Drops ◽  
Solution Ph ◽  
Zone Electrophoresis ◽  
Capillary Zone

A green, cost-effective, and simple capillary zone electrophoresis (CZE) method was developed and validated for simultaneous determination of chloramphenicol, methylparaben, and propylparaben in eye-drops. With sodium tetraborate as background electrolyte (BGE), the apparent mobilities of chloramphenicol, methylparaben, and propylparaben increased and analysis time reduced when pH of BGE increased from 8.5 to 10.0 and concentration of BGE decreased from 40 mM to 15 mM, but complete separation of chloramphenicol from other matrix components was achieved only with sodium tetraborate concentration at 30 mM or higher and at pH = 9.3 or lower. The most suitable electrophoretic conditions for the intended application were a 30 mM sodium tetraborate solution, pH 9.3 as BGE, working voltage set at 25 kV, and UV detection at 280 nm at the cathodic extremity of the capillary. The final method was validated and proved to be reliable for assay of chloramphenicol, methylparaben, and propylparaben in eye-drops.

A Capillary Zone Electrophoresis Method for Analysis of Glycosylated Recombinant Human Follicle-Stimulating Hormone

2019 ◽  
Vol 35 (4) ◽  
pp. 55-64
Author(s):  
M.E. Sushkin ◽  
A.S. Simbirtsev
Keyword(s):  
Sodium Chloride ◽  
Capillary Zone Electrophoresis ◽  
Protein Separation ◽  
Follicle Stimulating Hormone ◽  
Background Electrolyte ◽  
Sialic Acids ◽  
Zone Electrophoresis ◽  
Electrophoresis Method ◽  
Capillary Zone ◽  
Stimulating Hormone

Conditions for analyzing sialylated isoforms of recombinant follicle-stimulating hormone (FSH) by the method of capillary zone electrophoresis have been selected. Follicle-stimulating hormone is a glycosylated heterodimer with the large number of sialic acids that affect its basic biological and physicochemical properties. The optimum protein separation into 6 isoforms was achieved using as a background electrolyte of a solution with pH 4.3 containing 0.01 M trycin, 0.01 sodium chloride, 7 M urea and 2.5 mM 1,4-diaminobutane. follicle-stimulating hormone isoforms, capillary zone electrophoresis, sialic acids, follicle-stimulating hormone.

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