Two-stage Off-Gel? isoelectric focusing: Protein followed by peptide fractionation and application to proteome analysis of human plasma

2005 ◽  
Vol 26 (6) ◽  
pp. 1174-1188 ◽  
Author(s):  
Manfred Heller ◽  
Philippe E. Michel ◽  
Patrick Morier ◽  
David Crettaz ◽  
Christian Wenz ◽  
...  
Keyword(s):  
Human Plasma ◽  
Isoelectric Focusing ◽  
Proteome Analysis ◽  
Two Stage ◽  
Peptide Fractionation ◽  
Gel Isoelectric Focusing

Proteome analysis of human plasma and amniotic fluid by Off-Gel™ isoelectric focusing followed by nano-LC-MS/MS

2006 ◽  
Vol 27 (5-6) ◽  
pp. 1169-1181 ◽  
Author(s):  
Philippe E. Michel ◽  
David Crettaz ◽  
Patrick Morier ◽  
Manfred Heller ◽  
Denis Gallot ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Human Plasma ◽  
Isoelectric Focusing ◽  
Proteome Analysis

High pH reversed-phase chromatography as a superior fractionation scheme compared to off-gel isoelectric focusing for complex proteome analysis

PROTEOMICS ◽  
2013 ◽  
Vol 13 (20) ◽  
pp. 2956-2966 ◽  
Author(s):  
Derek R. Stein ◽  
Xiaojie Hu ◽  
Stuart J. McCorrister ◽  
Garrett R. Westmacott ◽  
Francis A. Plummer ◽  
...  
Keyword(s):  
Isoelectric Focusing ◽  
Proteome Analysis ◽  
Reversed Phase ◽  
Reversed Phase Chromatography ◽  
High Ph ◽  
Fractionation Scheme ◽  
Gel Isoelectric Focusing

Factor V from Human Plasma

1961 ◽  
Vol 05 (01) ◽  
pp. 001-020
Author(s):  
Douglas M. Surgenor ◽  
Nancy A. Wilson ◽  
Anne S. Henry
Keyword(s):  
Human Serum ◽  
Human Plasma ◽  
Kinetic Data ◽  
Human Factor ◽  
Kinetic Studies ◽  
Partial Purification ◽  
Factor V ◽  
Two Stage ◽  
Plasma Factor ◽  
Tissue Thromboplastin

SummaryA method is described for the partial purification of a human plasma factor which accelerates the conversion of prothrombin to thrombin in the presence of tissue thromboplastin. This factor may be dried from the frozen state, and may be kept in stable dry form for long periods of time. The quantitative assay of this activity is done in a classical two-stage prothrombin system using tissue thromboplastin and calcium. From its properties, it is concluded that this activity corresponds to factor V, labile factor and plasma Ac-globulin.Chemical and kinetic studies reveal that human factor V is active in plasma and is destroyed by thrombin. Human serum has little or no factor V activity.These results thus fail to support the postulated activation of factor V during clotting. All of the kinetic data are consistent with an enzymatic role for factor V in the formation of tissue prothrombin activator (thromboplastin).

Trimodal Mixed Mode Chromatography That Enables Efficient Offline Two-Dimensional Peptide Fractionation for Proteome Analysis

2017 ◽  
Vol 89 (17) ◽  
pp. 8884-8891 ◽  
Author(s):  
Peng Yu ◽  
Svenja Petzoldt ◽  
Mathias Wilhelm ◽  
Daniel Paul Zolg ◽  
Runsheng Zheng ◽  
...  
Keyword(s):  
Mixed Mode ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Peptide Fractionation

Identification of Australian barley cultivars by laboratory methods: gel electrophoresis and gel isoelectric focusing of the endosperm proteins

1977 ◽  
Vol 17 (89) ◽  
pp. 1020 ◽  
Author(s):  
J McCausland ◽  
CW Wrigley
Keyword(s):  
Isoelectric Focusing ◽  
Gel Electrophoresis ◽  
Water Soluble ◽  
The Other ◽  
Starch Gel Electrophoresis ◽  
Laboratory Methods ◽  
Barley Cultivars ◽  
Starch Gel ◽  
Electrophoretic Techniques ◽  
Gel Isoelectric Focusing

A range of laboratory methods was examined for their ability to distinguish between 19 barley cultivars currently grown in Australia. Aleurone colour, revealed after mechanical or chemical dehulling, differentiated Abyssinian, Atlas, Cape and Corvette from the other cultivars. Peroxidase and phenol testing were not useful. Seven different patterns were obtained for the hordeins of lowest mobility by starch gel electrophoresis. Further distinction was provided by flat gel isoelectric focusing of the water-soluble and hordein proteins for which 13 different pattern-groupings were obtained. The two electrophoretic techniques complemented one another, so that the use of both methods left only a few cultivars that could not be distinguished.

Sign in / Sign up

Export Citation Format

Share Document