Affinity capillary electrophoresis study of the linkage existing between proton and zinc ion binding to bacitracin A1

2003 ◽  
Vol 24 (5) ◽  
pp. 801-807 ◽  
Author(s):  
Massimo Castagnola ◽  
Diana Valeria Rossetti ◽  
Rosanna Inzitari ◽  
Alberto Vitali ◽  
Alessandro Lupi ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Zinc Ion ◽  
Ion Binding ◽  
Affinity Capillary Electrophoresis ◽  
Zinc Ion Binding

scholarly journals Crystal structure of the DENR-MCT-1 complex revealed zinc-binding site essential for heterodimer formation

2018 ◽  
Vol 116 (2) ◽  
pp. 528-533 ◽  
Author(s):  
Ivan B. Lomakin ◽  
Sergey E. Dmitriev ◽  
Thomas A. Steitz
Keyword(s):  
Crystal Structure ◽  
Binding Site ◽  
Translation Initiation ◽  
Zinc Ion ◽  
Zinc Binding ◽  
Ion Binding ◽  
Binding Interface ◽  
Zinc Binding Site ◽  
Reading Frames ◽  
Zinc Ion Binding

The density-regulated protein (DENR) and the malignant T cell-amplified sequence 1 (MCT-1/MCTS1) oncoprotein support noncanonical translation initiation, promote translation reinitiation on a specific set of mRNAs with short upstream reading frames, and regulate ribosome recycling. DENR and MCT-1 form a heterodimer, which binds to the ribosome. We determined the crystal structure of the heterodimer formed by human MCT-1 and the N-terminal domain of DENR at 2.0-Å resolution. The structure of the heterodimer reveals atomic details of the mechanism of DENR and MCT-1 interaction. Four conserved cysteine residues of DENR (C34, C37, C44, C53) form a classical tetrahedral zinc ion-binding site, which preserves the structure of the DENR’s MCT-1–binding interface that is essential for the dimerization. Substitution of all four cysteines by alanine abolished a heterodimer formation. Our findings elucidate further the mechanism of regulation of DENR-MCT-1 activities in unconventional translation initiation, reinitiation, and recycling.

Models for protein-zinc ion binding sites. II. The catalytic sites

2001 ◽  
Vol 83 (3-4) ◽  
pp. 150-165 ◽  
Author(s):  
David W. Deerfield ◽  
Charles W. Carter ◽  
Lee G. Pedersen
Keyword(s):  
Binding Sites ◽  
Zinc Ion ◽  
Ion Binding ◽  
Catalytic Sites ◽  
Ion Binding Sites ◽  
Zinc Ion Binding

Preparation, Properties and Analysis of a Zinc Ion-Binding Protein from the Benign Hypertrophic Human Prostate Gland

1973 ◽  
Vol 1 (1) ◽  
pp. 104-105
Author(s):  
M. JOAN REED ◽  
S. R. STITCH ◽  
J. G. HEATHCOTE ◽  
R. J. WASHINGTON
Keyword(s):  
Binding Protein ◽  
Prostate Gland ◽  
Zinc Ion ◽  
Human Prostate ◽  
Ion Binding ◽  
Zinc Ion Binding

scholarly journals A ratiometric fluorescent molecular probe with enhanced two-photon response upon Zn2+ binding for in vitro and in vivo bioimaging

2014 ◽  
Vol 5 (9) ◽  
pp. 3469-3474 ◽  
Author(s):  
Kizhmuri P. Divya ◽  
Sivaramapanicker Sreejith ◽  
Pichandi Ashokkumar ◽  
Kang Yuzhan ◽  
Qiwen Peng ◽  
...  
Keyword(s):  
Zinc Ion ◽  
Molecular Probe ◽  
Ion Binding ◽  
Ion Imaging ◽  
Two Photon ◽  
Zinc Ion Binding

The ratiometric two-photon (2P) probe GBC shows enhanced 2P activity upon zinc ion binding and has been used for zinc ion imaging in vitro and in vivo.

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