Practical integration of polymerase chain reaction amplification and electrophoretic analysis in microfluidic devices for genetic analysis

2003 ◽  
Vol 24 (12) ◽  
pp. 172-178 ◽  
Author(s):  
Isabel Rodriguez ◽  
Marie Lesaicherre ◽  
Yan Tie ◽  
Quanbo Zou ◽  
Chen Yu ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Genetic Analysis ◽  
Polymerase Chain Reaction Amplification ◽  
Microfluidic Devices ◽  
Electrophoretic Analysis ◽  
Chain Reaction ◽  
Reaction Amplification ◽  
Polymerase Chain

Polymerase chain reaction amplification and typing of rotavirus in environmental samples

1995 ◽  
Vol 31 (5-6) ◽  
pp. 371-374 ◽  
Author(s):  
R. Gajardo ◽  
R. M. Pintó ◽  
A. Bosch
Keyword(s):  
Polymerase Chain Reaction ◽  
Environmental Samples ◽  
Polymerase Chain Reaction Amplification ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Group A ◽  
Reaction Amplification ◽  
Stool Specimens ◽  
Polymerase Chain

A reverse transcription polymerase chain reaction (RT-PCR) assay is described that has been developed for the detection and serotyping of group A rotavirus in stool specimens and concentrated and non-concentrated sewage specimens.

Molecular Basis for Functional Differences of AMPA-Subtype Glutamate Receptors

Physiology ◽  
1996 ◽  
Vol 11 (2) ◽  
pp. 77-82 ◽  
Author(s):  
S Ozawa ◽  
J Rossier
Keyword(s):  
Polymerase Chain Reaction ◽  
Ampa Receptors ◽  
Molecular Basis ◽  
Hippocampal Neurons ◽  
Polymerase Chain Reaction Amplification ◽  
Inward Rectification ◽  
Chain Reaction ◽  
Functional Differences ◽  
Reaction Amplification ◽  
Polymerase Chain

To trace the molecular basis of functional properties of native a-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) receptors, we have coupled patch-clamp recordings and reverse transcription followed by polymerase chain reaction amplification. AMPA receptors lacking the GluR2 subunit in a population of hippocampal neurons exhibited a strong inward rectification and were highly permeable to Ca2+.

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