Capillary electrophoresis of cationic random coil peptide standards: Effect of anionic ion-pairing reagents and comparison with reversed-phase chromatography

2004 ◽  
Vol 25 (9) ◽  
pp. 1219-1229 ◽  
Author(s):  
Traian V. Popa ◽  
Colin T. Mant ◽  
Robert S. Hodges
Keyword(s):  
Capillary Electrophoresis ◽  
Ion Pairing ◽  
Reversed Phase ◽  
Random Coil ◽  
Reversed Phase Chromatography

scholarly journals Determination of DiazaCon in Quail Feed and Quail Serum by Ion Pair Reversed-Phase Chromatography

2001 ◽  
Vol 84 (3) ◽  
pp. 634-639 ◽  
Author(s):  
John J Johnston ◽  
Margaret J Goodall ◽  
Jerome C Hurley ◽  
Christi A Yoder ◽  
Lowell A Miller
Keyword(s):  
Aqueous Solution ◽  
Ion Pairing ◽  
Reversed Phase ◽  
Ion Pair ◽  
Butyl Chloride ◽  
Reversed Phase Chromatography ◽  
Limits Of Detection ◽  
The Mean ◽  
Liquid Chromatographic

Abstract Liquid chromatographic (LC) methods were developed for quantitating the potential avian contraceptive DiazaCon in quail feed and serum. DiazaCon was extracted from ground quail feed with basic n-butyl chloride. The n-butyl chloride extract was evaporated to dryness. The DiazaCon residues were dissolved in an aqueous methanolic ion pairing solution and quantitated by LC at 206 nm. Avian sera was combined with an equal volume of a pH 4 aqueous solution of ion pairing reagent and filtered to remove interfering proteins. DiazaCon was quantitated by LC. Mean recoveries for 500 and 2000 ppm fortified feed were 89.1 and 91.0%, respectively. The mean recovery for sera fortified at 5 levels ranging from 35 to 2000 ppm was 84.9%. Method limits of detection were approximately 14 and 13 ppm for feed and sera, respectively.

The role of ion-pairing in peak deformations in overloaded reversed-phase chromatography of peptides

2010 ◽  
Vol 1217 (45) ◽  
pp. 7065-7073 ◽  
Author(s):  
Abhijit Tarafder ◽  
Lars Aumann ◽  
Massimo Morbidelli
Keyword(s):  
Ion Pairing ◽  
Reversed Phase ◽  
Reversed Phase Chromatography

Modeling of ion-pairing effect in peptide reversed-phase chromatography

2012 ◽  
Vol 1249 ◽  
pp. 92-102 ◽  
Author(s):  
David Gétaz ◽  
Subrahmaniam B. Hariharan ◽  
Alessandro Butté ◽  
Massimo Morbidelli
Keyword(s):  
Ion Pairing ◽  
Reversed Phase ◽  
Reversed Phase Chromatography ◽  
Pairing Effect

Isocratic separation of ATP and its degradation products from biological fluids by automated liquid chromatography.

1988 ◽  
Vol 34 (5) ◽  
pp. 925-932 ◽  
Author(s):  
K K Tekkanat ◽  
I H Fox
Keyword(s):  
Liquid Chromatography ◽  
Degradation Products ◽  
Biological Fluids ◽  
Ion Pairing ◽  
Reversed Phase ◽  
Reversed Phase Chromatography ◽  
Group A ◽  
Group B

Abstract Two groups of metabolites (a) IMP, AMP, ADP, ATP, and cAMP in extracts of fibroblasts and erythrocytes and (b) hypoxanthine, xanthine, adenosine, and inosine in plasma and urine have been separated by ion-pairing reversed-phase chromatography on a microBondapak C18 column, with use of the following reagents: 60 mmol/L KH2PO4, 0.45 mmol/L tetrabutylammonium phosphate, and 1.26 mol/L acetonitrile, pH 3.2 (at 23 degrees C) (group a) and 20 mmol/L KH2PO4, 0.45 mmol/L tetrabutylammonium phosphate, and 0.35 mol/L acetonitrile, pH 2.70 (at 24 degrees C) (group b). Under both sets of conditions, the compounds are completely separated in less than 15 min. The separation is isocratic, so the method is easily adaptable to automation.

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