Enantioselective strong cation-exchange molecular recognition materials: Design of novel chiral stationary phases and their application for enantioseparation of chiral bases by nonaqueous capillary electrochromatography

2003 ◽  
Vol 24 (10) ◽  
pp. 1668-1679 ◽  
Author(s):  
Stéphane Constantin ◽  
Wolfgang Bicker ◽  
Elfriede Zarbl ◽  
Michael Lämmerhofer ◽  
Wolfgang Lindner
Keyword(s):  
Molecular Recognition ◽  
Cation Exchange ◽  
Capillary Electrochromatography ◽  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Materials Design ◽  
Strong Cation Exchange

Macroporous monolithic chiral stationary phases for capillary electrochromatography: New chiral monomer derived from cinchona alkaloid with enhanced enantioselectivity

2003 ◽  
Vol 24 (17) ◽  
pp. 2986-2999 ◽  
Author(s):  
Michael Lämmerhofer ◽  
Ernst Tobler ◽  
Elfriede Zarbl ◽  
Wolfgang Lindner ◽  
Frantisek Svec ◽  
...  
Keyword(s):  
Capillary Electrochromatography ◽  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Cinchona Alkaloid

scholarly journals Comparison of Various Chromatographic Systems for Analysis of Cytisine in Human Serum, Saliva and Pharmaceutical Formulation by HPLC with Diode Array, Fluorescence or Mass Spectrometry Detection

Molecules ◽  
2019 ◽  
Vol 24 (14) ◽  
pp. 2580 ◽  
Author(s):  
Karol Wróblewski ◽  
Anna Petruczynik ◽  
Tomasz Tuzimski ◽  
Dominika Przygodzka ◽  
Grzegorz Buszewicz ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Cation Exchange ◽  
Biological Samples ◽  
Stationary Phases ◽  
Reversed Phase ◽  
Pharmaceutical Formulation ◽  
Diode Array ◽  
Strong Cation Exchange ◽  
Pre Treatment

Background: Identification and quantitative determination of cytisine, especially in biological samples and pharmaceutical formulations, is still a difficult analytical task. Cytisine is an alkaloid with a small and very polar molecule. For this reason, it is very weakly retained on reversed phase (RP) stationary phases, such as commonly used alkyl-bonded phases. The very weak retention of cytisine causes it to be eluted together with the components of biological matrices. Objective: Comparison and evaluation of various chromatographic systems for analysis of cytisine in different matrices—serum, saliva and pharmaceutical formulation—by high performance liquid chromatography (HPLC) with diode array (DAD), fluorescence (FLD) and mass spectrometry (MS) detection. Methods: The analyses were performed using HPLC in reversed phase (RP), hydrophilic interaction liquid chromatography (HILIC) and ion exchange chromatography (IEC) modes. Different sample pre-treatment methods were tested: Protein precipitation (with acetone, methanol (MeOH) or acetonitrile (ACN), and solid phase extraction (SPE) using cartridges with octadecyl (C18), hydrophilic-lipophilic balanced copolymer (HLB) or strong cation exchange sorbents (Strata X-C). Conclusion: Significant differences were observed in retention parameters with a change of the used chromatographic system. The various properties of stationary phases resulted in differences in analyte retention, peaks’ shape and systems’ efficiency. The weakest retention was observed using RP systems; however, the use of the Polar RP phase can be an alternative for application in green chromatography. In the strongest retention was observed using a strong cation exchange (SCX) phase. The most optimal systems were chosen for the analysis of cytisine in the pharmaceutical preparation, serum and saliva after sample pre-treatment with the new SPE procedure. Due to the sensitivity, the use of HPLC-DAD or HPLC-FLD is the most optimal for drug analysis in pharmaceutical preparations, whereas HPLC-MS is suitable for analysis of cytisine in biological samples.

scholarly journals Electrochromatographic retention of peptides on strong cation-exchange stationary phases

2010 ◽  
Vol 31 (5) ◽  
pp. 933-943 ◽  
Author(s):  
Ivo Nischang ◽  
Alexandra Höltzel ◽  
Ulrich Tallarek
Keyword(s):  
Cation Exchange ◽  
Stationary Phases ◽  
Strong Cation Exchange

Synthesis of cyclam-capped β-cyclodextrin-bonded silica particles for use as chiral stationary phases in capillary electrochromatography

2002 ◽  
Vol 43 (13) ◽  
pp. 2463-2466 ◽  
Author(s):  
Yinhan Gong ◽  
Guoping Xue ◽  
Yanqiao Xiang ◽  
Jerald S Bradshaw ◽  
Milton L Lee ◽  
...  
Keyword(s):  
Capillary Electrochromatography ◽  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Silica Particles
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