Optimized determination of carbohydrate-deficient transferrin isoforms in serum by capillary zone electrophoresis

1998 ◽  
Vol 19 (16-17) ◽  
pp. 3033-3039 ◽  
Author(s):  
Franco Tagliaro ◽  
Federica Crivellente ◽  
Giulia Manetto ◽  
Ivana Puppi ◽  
Zdenek Deyl ◽  
...  
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Zone Electrophoresis ◽  
Carbohydrate Deficient Transferrin ◽  
Capillary Zone ◽  
Transferrin Isoforms

Capillary zone electrophoresis for determination of carbohydrate-deficient transferrin in human serum

2004 ◽  
Vol 25 (3) ◽  
pp. 469-475 ◽  
Author(s):  
Isabella Fermo ◽  
Luca Germagnoli ◽  
Armando Soldarini ◽  
Fernanda Dorigatti ◽  
Rita Paroni
Keyword(s):  
Human Serum ◽  
Capillary Zone Electrophoresis ◽  
Zone Electrophoresis ◽  
Carbohydrate Deficient Transferrin ◽  
Capillary Zone

scholarly journals Determination of carbohydrate-deficient transferrin in human serum with capillary zone electrophoresis

2008 ◽  
Vol 1206 (1) ◽  
pp. 33-40 ◽  
Author(s):  
Christian Lanz ◽  
Jean-Bernard Falmagne ◽  
François de l’Escaille ◽  
Ulrich Marti ◽  
Wolfgang Thormann
Keyword(s):  
Human Serum ◽  
Capillary Zone Electrophoresis ◽  
Zone Electrophoresis ◽  
Carbohydrate Deficient Transferrin ◽  
Capillary Zone

scholarly journals Carbohydrate-deficient Transferrin Isoforms Measured by Capillary Zone Electrophoresis for Detection of Alcohol Abuse

2002 ◽  
Vol 48 (12) ◽  
pp. 2177-2186 ◽  
Author(s):  
Franz J Legros ◽  
Vincent Nuyens ◽  
Eddy Minet ◽  
Philippe Emonts ◽  
Karim Zouaoui Boudjeltia ◽  
...  
Keyword(s):  
Alcohol Abuse ◽  
Capillary Zone Electrophoresis ◽  
Fused Silica ◽  
Alcohol Abusers ◽  
Zone Electrophoresis ◽  
Carbohydrate Deficient Transferrin ◽  
Capillary Zone ◽  
Roc Area ◽  
Improving Accuracy ◽  
Transferrin Isoforms

Abstract Background: Measurements of carbohydrate-deficient transferrin (CDT) are used as markers of alcohol abuse. We developed a capillary zone electrophoresis (CZE) method aimed at improving accuracy of CDT testing. Methods: We studied 111 alcohol abusers with Alcohol Use Disorders Identification Test scores >11 and 50 teetotalers. CZE was performed with a P/ACE 5500, fused-silica capillaries, and a CEofix CDT reagent set. After iron saturation, sera were loaded by low-pressure injection, separated at 28 kV, and monitored at 214 nm. We identified the transferrin isoforms by migration times, treatment with 100 U/L neuraminidase, and immunosubtraction with anti-human transferrin and anti-C-reactive protein antibodies. We compared CZE results with current biological markers of alcohol abuse, including the %CDT turbidimetric immunoassay. Results: Migration times of the isoforms were identical in both populations. Asialotransferrin was missing in teetotalers but present in 92% of alcohol abusers. Disialotransferrin was higher in those who consumed excessive amounts of alcohol, whereas mean trisialotransferrin concentration was not affected by alcohol abuse. At cutoffs to maximize sensitivity and specificity, these values were 0.92 and 1 [mean ROC area (MRa), 0.96; 95% confidence interval (CI), 0.93–0.99] for asialotransferrin; 0.84 and 0.94 for the sum of asialo- + disialotransferrin (MRa, 0.94; 95% CI, 0.91–0.98); 0.79 and 0.94 for disialotransferrin (MRa, 0.89; 95% CI, 0.84–0.94); 0.62 and 0.53 for trisialotransferrin (MRa, 0.58; 95% CI, 0.49–0.68); 0.79 and 0.82 for a 3% %CDT; and 0.83 and 0.69 for a 2.6% cutoff (MRa, 0.87; 95% CI, 0.81–0.92). Current markers lack sensitivity (<0.65). Transferrins were not significantly correlated with serum enzymes and mean erythrocyte volume. Conclusions: CZE-isolated desialylated transferrin isoforms allowed differentiation between chronic alcohol abusers and teetotalers.

Transferrin immunoextraction for determination of carbohydrate-deficient transferrin in human serum by capillary zone electrophoresis

2012 ◽  
Vol 35 (24) ◽  
pp. 3521-3528 ◽  
Author(s):  
Jitka Caslavska ◽  
Jeannine Joneli ◽  
Ursula Wanzenried ◽  
Jeannette Schiess ◽  
Wolfgang Thormann
Keyword(s):  
Human Serum ◽  
Capillary Zone Electrophoresis ◽  
Zone Electrophoresis ◽  
Carbohydrate Deficient Transferrin ◽  
Capillary Zone
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