Proteins of rat serum: I. Establishing a reference two-dimensional electrophoresis map by immunodetection and microbore high performance liquid chromatography-electrospray mass spectrometry

1998 ◽  
Vol 19 (8-9) ◽  
pp. 1484-1492 ◽  
Author(s):  
Paul Haynes ◽  
Ingrid Miller ◽  
Ruedi Aebersold ◽  
Manfred Gemeiner ◽  
Ivano Eberini ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Electrospray Mass Spectrometry ◽  
Two Dimensional ◽  
Rat Serum ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

scholarly journals Empowering Shotgun Mass Spectrometry with 2DE: A HepG2 Study

2020 ◽  
Vol 21 (11) ◽  
pp. 3813
Author(s):  
Olga Kiseleva ◽  
Victor Zgoda ◽  
Stanislav Naryzhny ◽  
Ekaterina Poverennaya
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Two Dimensional ◽  
Rna Seq ◽  
Two Dimensional Electrophoresis ◽  
Shotgun Mass Spectrometry ◽  
Human Proteome Project ◽  
Dimensional Electrophoresis ◽  
Hepatoblastoma Cell Line

One of the major goals of the Chromosome-Centric Human Proteome Project (C-HPP) is to catalog and annotate a myriad of heterogeneous proteoforms, produced by ca. 20 thousand genes. To achieve a detailed and personalized understanding into proteomes, we suggest using a customized RNA-seq library of potential proteoforms, which includes aberrant variants specific to certain biological samples. Two-dimensional electrophoresis coupled with high-performance liquid chromatography allowed us to downgrade the difficulty of biological mixing following shotgun mass spectrometry. To benchmark the proposed pipeline, we examined heterogeneity of the HepG2 hepatoblastoma cell line proteome. Data are available via ProteomeXchange with identifier PXD018450.

Simultaneous determination of albendazole metabolites, praziquantel and its metabolite in plasma by high-performance liquid chromatography–electrospray mass spectrometry

2007 ◽  
Vol 44 (2) ◽  
pp. 558-563 ◽  
Author(s):  
Pierina Sueli Bonato ◽  
Anderson Rodrigo Moraes de Oliveira ◽  
Fernando José Malagueño de Santana ◽  
Bruno José Dumet Fernandes ◽  
Vera Lucia Lanchote ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Electrospray Mass Spectrometry
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