Characterization ofChlamydia trachomatis L2-induced tyrosine-phosphorylated HeLa cell proteins by two-dimensional gel electrophoresis

1997 ◽  
Vol 18 (3-4) ◽  
pp. 563-567 ◽  
Author(s):  
Svend Birkelund ◽  
Luca Bini ◽  
Vitaliano Pallini ◽  
Maria Sanchez-Campillo ◽  
Sabrina Liberatori ◽  
...  
Keyword(s):  
Hela Cell ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Ofchlamydia Trachomatis ◽  
Two Dimensional Gel Electrophoresis

scholarly journals The synthesis of ninety proteins including actin throughout the HeLa cell cycle.

1978 ◽  
Vol 79 (3) ◽  
pp. 833-838 ◽  
Author(s):  
C Milcarek ◽  
K Zahn
Keyword(s):  
Cell Cycle ◽  
Total Synthesis ◽  
Hela Cell ◽  
Gel Electrophoresis ◽  
Similar Proportion ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Time Points ◽  
Cytoplasmic Proteins ◽  
Actin Protein

Abundant cytoplasmic proteins pulse-labeled with [35S]methionine at specific times throughout the HeLa cell cycle were analyzed with two-dimensional gel electrophoresis. More than 300 proteins could be resolved in this way. The frequency of appearance of label in the most abundant 90 proteins, ranging from 4% to less than 0.1% of the total methionine incorporated, was determined at six time points in the cell cycle. 84 of these proteins were made as a similar proportion of the total at all times during the cell cycle. A nonmuscle actin protein (spot 1) identified by molecular weight and isoelectric point represented 2-4% of the total methionine incorporated at all the time points. Only six proteins were found which varied by greater than fourfold during cell division, four appearing to represent a greater proportion of the total synthesis during the period at or immediately surrounding M (spots 31b, 44, 53, and 70d). Two appear to represent a smaller percentage of total synthesis during the early (spot 78) or the total (spot 74) G2 period.

Mapping and identification of interferon gammaregulated HeLa cell proteins separated by immobilized pH gradient two-dimensional gel electrophoresis

2007 ◽  
pp. 404-413
Author(s):  
Allan Christian Shaw ◽  
Martin Rssel Larsen ◽  
Peter Roepstorff ◽  
Just Justesen ◽  
Gunna Christiansen ◽  
...  
Keyword(s):  
Hela Cell ◽  
Gel Electrophoresis ◽  
Ph Gradient ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

Mapping and identification of interferon gamma-regulated HeLa cell proteins separated by immobilized pH gradient two-dimensional gel electrophoresis

1999 ◽  
Vol 20 (4-5) ◽  
pp. 984-993 ◽  
Author(s):  
Allan Christian Shaw ◽  
Martin Røssel Larsen ◽  
Peter Roepstorff ◽  
Just Justesen ◽  
Gunna Christiansen ◽  
...  
Keyword(s):  
Hela Cell ◽  
Interferon Gamma ◽  
Gel Electrophoresis ◽  
Ph Gradient ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

Diversity of Glycoprotein Deficiencies in Glanzmann’s Thrombasthenia

1985 ◽  
Vol 54 (03) ◽  
pp. 626-629 ◽  
Author(s):  
M Meyer ◽  
F H Herrmann
Keyword(s):  
High Resolution ◽  
Gel Electrophoresis ◽  
Type Ii ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Structural Variant ◽  
Glanzmann’S Thrombasthenia ◽  
Crossed Immunoelectrophoresis ◽  
Glanzmann's Thrombasthenia ◽  
Platelet Proteins

SummaryThe platelet proteins of 9 thrombasthenic patients from 7 families were analysed by high resolution two-dimensional gel electrophoresis (HR-2DE) and crossed immunoelectrophoresis (CIE). In 7 patients both glycoproteins (GPs) IIb and Ilia were absent or reduced to roughly the same extent. In two related patients only a trace of GP Ilb-IIIa complex was detected in CIE, but HR-2DE revealed a glycopeptide in the position of GP Ilia in an amount comparable to type II thrombasthenia. This GP Ilia-like component was neither recognized normally by anti-GP Ilb-IIIa antibodies nor labeled by surface iodination. In unreduced-reduced two-dimensional gel electrophoresis two components were observed in the region of GP Ilia. The assumption of a structural variant of GP Ilia in the two related patients is discussed.

Comparative Two-Dimensional Gel Electrophoresis of Trypanosoma cruzi Mammalian-Stage Forms in an Alkaline pH Range

2015 ◽  
Vol 22 (12) ◽  
pp. 1066-1075 ◽  
Author(s):  
Adriana Magalhães ◽  
Rayner Queiroz ◽  
Izabela Bastos ◽  
Jaime Santana ◽  
Marcelo Sousa ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Alkaline Ph ◽  
Two Dimensional Gel Electrophoresis ◽  
Ph Range

Proteome Analysis of CD4+ T Cells Reveals Differentially Expressed Proteins in Infertile Polycystic Ovary Syndrome Patients

2020 ◽  
Vol 20 ◽  
Author(s):  
Fatemeh Nasri ◽  
Maryam Zare ◽  
Mehrnoosh Doroudchi ◽  
Behrouz Gharesi-Fard
Keyword(s):  
Mass Spectrometry ◽  
T Cells ◽  
T Lymphocytes ◽  
Gel Electrophoresis ◽  
Cd4 T Cells ◽  
Polycystic Ovary ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Ovary Syndrome ◽  
Two Dimensional Gel Electrophoresis

Background: Polycystic ovary syndrome (PCOS) is the most frequent endocrine disorder affecting 6–7% of premenopausal women. Recent studies revealed that the immune system especially CD4+ T helper cells are important in the context PCOS. Proteome analysis of CD4+ T lymphocytes can provide valuable information regarding the biology of these cells in the context of PCOS. Objective: To investigate immune dysregulation in CD4+ T lymphocytes at the protein level in the context of PCOS using two-dimensional gel electrophoresis (2DE) and mass spectrometry (MS). Methods: In the present study, we applied two-dimensional gel electrophoresis / mass spectrometry to identify proteins differentially expressed by peripheral blood CD4+ T cells in ten PCOS women compared with ten healthy women. Western blot technique was used to confirm the identified proteins. Results: Despite the overall proteome similarities, there were significant differences in the expression of seven spots between two groups (P <0.05). Three proteins, namely phosphatidylethanolamine-binding protein 1, proteasome activator complex subunit 1 and triosephosphate isomerase 1 were successfully identified by Mass technique and confirmed by western blot. All characterized proteins were over-expressed in CD4+ T cells from patients compared to CD4+ T cells from controls (P <0.05). In-silico analysis suggested that the over-expressed proteins interact with other proteins involved in cellular metabolism especially glycolysis and ferroptosis pathway. Conclusion: These findings suggest that metabolic adjustments in CD4+ T lymphocytes, which is in favor of increased glycolysis and Th2 differentiation are important in the context of PCOS.

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