Electrophoretic analysis of biliary proteins: Application of high resolution two-dimensional polyacrylamide gel electrphoresis with immobilized pH gradient in the first dimension

1996 ◽  
Vol 17 (3) ◽  
pp. 617-619 ◽  
Author(s):  
Chang He ◽  
Dieter Jüngst
Keyword(s):  
High Resolution ◽  
Electrophoretic Analysis ◽  
Polyacrylamide Gel ◽  
Ph Gradient ◽  
Two Dimensional

Two-dimensional electrophoretic analysis of cytosols from human breast tumors: optimal migration conditions.

1984 ◽  
Vol 30 (12) ◽  
pp. 1947-1949 ◽  
Author(s):  
P Motté ◽  
J M Bidart ◽  
J C Delarue ◽  
E Comoy ◽  
P Moingeon ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Breast Tumors ◽  
Electrophoretic Analysis ◽  
Ph Gradient ◽  
Two Dimensional ◽  
Human Breast ◽  
Cytosolic Proteins ◽  
Optimal Migration ◽  
Dodecyl Sulfate

Abstract In an examination of cytosols from human breast tumors, we performed two-dimensional electrophoretic analysis. Several migration conditions were tried in a search for a homogeneous repartition of cytosolic proteins. The most workable gels were obtained with a 4.40 to 8.05 pH gradient in the first dimension and a homogeneous 125 g/L acrylamide/sodium dodecyl sulfate gel, 1 mm in thickness, in the second dimension. The practicability of this method is discussed.

Nonreducing two-dimensional polyacrylamide gel electrophoretic analysis of human colonic proteins

1995 ◽  
Vol 16 (1) ◽  
pp. 1120-1130 ◽  
Author(s):  
Gavin E. Reid ◽  
Hong Ji ◽  
James S. Eddes ◽  
Robert L. Moritz ◽  
Richard J. Simpson
Keyword(s):  
Electrophoretic Analysis ◽  
Polyacrylamide Gel ◽  
Two Dimensional
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