Two-dimensional electrophoretic patterns of acute-phase human serum proteins in the course of bacterial and viral diseases

1996 ◽  
Vol 17 (3) ◽  
pp. 612-616 ◽  
Author(s):  
Luca Bini ◽  
Barbara Magi ◽  
Barbara Marzocchi ◽  
Carla Cellesi ◽  
Brunilde Berti ◽  
...  
Keyword(s):  
Human Serum ◽  
Acute Phase ◽  
Serum Proteins ◽  
Viral Diseases ◽  
Two Dimensional ◽  
Electrophoretic Patterns ◽  
Human Serum Proteins

Two-dimensional electrophoresis analysis of human serum proteins during the acute-phase response

1992 ◽  
Vol 13 (1) ◽  
pp. 743-746 ◽  
Author(s):  
Luca Bini ◽  
Barbara Magi ◽  
Carla Cellesi ◽  
Aldo Rossolini ◽  
Vitaliano Pallini
Keyword(s):  
Human Serum ◽  
Acute Phase ◽  
Acute Phase Response ◽  
Serum Proteins ◽  
Phase Response ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis ◽  
Human Serum Proteins

Two-dimensional electrophoresis of human serum proteins modified by ampicillin during therapeutic treatment

1995 ◽  
Vol 16 (1) ◽  
pp. 1190-1192 ◽  
Author(s):  
Barbara Magi ◽  
Barbara Marzocchi ◽  
Luca Bini ◽  
Carla Cellesi ◽  
Aldo Rossolini ◽  
...  
Keyword(s):  
Human Serum ◽  
Serum Proteins ◽  
Two Dimensional ◽  
Therapeutic Treatment ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis ◽  
Human Serum Proteins

Direct chemiluminescent imaging detection of human serum proteins in two-dimensional polyacrylamide gel electrophoresis

PROTEOMICS ◽  
2007 ◽  
Vol 7 (19) ◽  
pp. 3481-3490 ◽  
Author(s):  
Qian Tao ◽  
Zhenzhen Wang ◽  
Heping Zhao ◽  
Willy R. G. Baeyens ◽  
Joris R. Delanghe ◽  
...  
Keyword(s):  
Human Serum ◽  
Gel Electrophoresis ◽  
Serum Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Two Dimensional ◽  
Imaging Detection ◽  
Human Serum Proteins

Rapid Two-Dimensional Immunoelectrophoresis of Human Serum Proteins

1974 ◽  
Vol 20 (1) ◽  
pp. 30-35 ◽  
Author(s):  
G L Wright ◽  
Louis Pollack ◽  
D B Roberts
Keyword(s):  
Human Serum ◽  
Serum Proteins ◽  
Clinical Laboratory ◽  
Rapid Screening ◽  
Two Dimensional ◽  
Special Apparatus ◽  
Time Required ◽  
Laboratory Time ◽  
Sensitive Method ◽  
Human Serum Proteins

Abstract We describe two versions of a two-dimensional immunoelectrophoretic system and illustrate their use for separating and estimating human serum proteins. Each is sensitive, rapid, and simple to perform, and requires no special apparatus and only 0.1-0.6 ml of antiserum. Both procedures appear to be potentially useful in the clinical laboratory. Time required to obtain a stained pattern varies from 4.5 to 7 h, depending on the intended purpose and sensitivity (number of possible precipitin peaks) desired. With the faster method ("rapid screening") 25 to 35 precipitin peaks are detected. With the slower but more sensitive method 40 to > 50 peaks are detected. The sensitivity of either version is a function of the titer of the polyvalent antiserum to whole human serum. Of the precipitin peaks thus far detected, 35 have been identified.

Sign in / Sign up

Export Citation Format

Share Document