Molecular epidemiology of respiratory syncytial virus: A review of the use of reverse transcription-polymerase chain reaction in the analysis of genetic variability

1995 ◽  
Vol 16 (1) ◽  
pp. 329-333 ◽  
Author(s):  
Patricia A. Cane ◽  
Craig R. Pringle
Keyword(s):  
Polymerase Chain Reaction ◽  
Respiratory Syncytial Virus ◽  
Genetic Variability ◽  
Molecular Epidemiology ◽  
Reverse Transcription ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Syncytial Virus

Midturbinate Swabs Are Comparable to Nasopharyngeal Swabs for Quantitative Detection of Respiratory Syncytial Virus in Infants

2018 ◽  
Vol 8 (6) ◽  
pp. 554-558 ◽  
Author(s):  
Anne J Blaschke ◽  
Matt McKevitt ◽  
Krow Ampofo ◽  
Tammi Lewis ◽  
Hao Chai ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Respiratory Syncytial Virus ◽  
Real Time ◽  
Reverse Transcription ◽  
Quantitative Polymerase Chain Reaction ◽  
Quantitative Detection ◽  
Chain Reaction ◽  
Viral Loads ◽  
Polymerase Chain ◽  
Syncytial Virus

Abstract Nasopharyngeal (NP) swabs are generally used to detect respiratory syncytial virus (RSV) in infants. However, midturbinate (MT) swabs may provide comparable results. In this study, we enrolled hospitalized infants aged <24 months with RSV and collected NP and MT swabs. The resulting viral loads measured by real-time reverse-transcription quantitative polymerase chain reaction were similar. Most parents preferred MT swabs over NP swabs.

Detection of Genomic Sequences of Respiratory Syncytial Virus in Otitis Media with Effusion in Children

1992 ◽  
Vol 101 (10_suppl) ◽  
pp. 7-10 ◽  
Author(s):  
Yoshitaka Okamoto ◽  
Kazuo Kudo ◽  
Koji Shirotori ◽  
Misao Nakazawa ◽  
Eiko Ito ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Respiratory Syncytial Virus ◽  
Otitis Media ◽  
Otitis Media With Effusion ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Rsv Infection ◽  
Polymerase Chain ◽  
Syncytial Virus ◽  
Viral Sequences

The reverse transcriptase—polymerase chain reaction and the nested polymerase chain reaction were used for detection of respiratory syncytial virus (RSV) sequences in middle ear effusions collected from children with otitis media. Sequences of RSV were detected in 21 of 34 samples tested. These samples were collected during and/or after natural outbreaks of RSV infection in the community. In those patients from whose nasopharynges RSV was isolated, the viral sequences were highly detectable (75%) in the effusions. These observations suggest RSV as an important factor in the pathogenesis of otitis media with effusion.

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