Cationic detergent in agarose for improved electrophoresis of cationic proteins

1991 ◽  
Vol 12 (1) ◽  
pp. 96-99 ◽  
Author(s):  
Claus M. Reimert ◽  
Thorkild C. Bøg-Hansen
Keyword(s):  
Cationic Proteins ◽  
Cationic Detergent

Application of RT-PCR for the Detection of Enteric Viruses in Oysters

1993 ◽  
Vol 27 (3-4) ◽  
pp. 49-53 ◽  
Author(s):  
L. A. Jaykus ◽  
R. De Leon ◽  
M. D. Sobsey
Keyword(s):  
Enteric Viruses ◽  
Rt Pcr ◽  
Chloroform Extraction ◽  
Cationic Detergent

Oyster samples processed by adsorption-elution-precipitation were seeded with poliovirus 1 and HAV, and cleaned and concentrated by Freon extraction (2X), PEG precipitation and chloroform extraction. Freon extraction resulted in recoveries of 63-76% for polio and 42-52% for HAV. PEG precipitation/chloroform extraction gave recoveries of 47-50% for polio and 15-19% for HAV. Treated extracts inhibited RT-PCR at 10−2 dilutions. Inhibitors were removed by treatment with the cationic detergent CTAB or Pro-Cipitate/UF adsorption-elution-concentration. Both treatments resulted in samples on which direct RT-PCR was possible. The CTAB procedure was able to detect 78 pfu of polio and 295 pfu of HAV. The Pro-Cipitate procedure was able to detect 70 pfu polio and 2.1×103 pfu HAV.

scholarly journals POLYMORPHONUCLEAR LEUKOCYTES IN IMMUNOLOGIC REACTIONS

1966 ◽  
Vol 124 (4) ◽  
pp. 733-752 ◽  
Author(s):  
Charles G. Cochrane ◽  
Barbara S. Aikin
Keyword(s):  
Basement Membrane ◽  
Polymorphonuclear Leukocytes ◽  
Electron Microscopic Observation ◽  
Severe Damage ◽  
Electron Microscopic ◽  
Cationic Proteins ◽  
Vascular Basement Membrane ◽  
Arthus Phenomenon ◽  
Immunologic Reactions

Vascular basement membrane was disrupted in the presence of polymorphonuclear leukocytes (PMN's) during two immunologic reactions: The Arthus phenomenon and the reaction to locally injected antibody to vascular basement membrane. This disruption was evidenced by (a) the inability of the basement membrane to retain circulating carbon, by (b) loss of antigenic constituents, and by (c) electron microscopic observation showing actual gaps in the structure of the vascular basement membrane. The factors within PMN's responsible for damage to isolated glomerular basement membrane in vitro were found by isolation procedures to be cathepsins D and E. Cationic proteins of PMN's were separable from the cathepsins. While inducing vascular permeability upon injection, these basic proteins failed to inflict the severe damage to the basement membrane observed in Arthus and antibasement membrane reactions. It is concluded that the full expression of these immunologic lesions requires destruction of the basement membrane possibly brought about by cathepsins D and E. Some of the physicochemical properties of these pathologically active leukocytic factors are given.

scholarly journals Effect of Albumin and Detergent on the Reduction of NBT by 1-Deoxy-1-Morpholinofructose

1989 ◽  
Vol 26 (6) ◽  
pp. 496-499 ◽  
Author(s):  
E Podzorski ◽  
F E Wells
Keyword(s):  
Molecular Weight ◽  
Glycated Albumin ◽  
Low Molecular Weight ◽  
Albumin Concentration ◽  
Protein Matrix ◽  
Detergent Concentration ◽  
Low Concentration ◽  
Cationic Detergent ◽  
Kinetic Effects

The effect of matrix albumin concentration on reduction of NBT by DMF has been investigated together with the effect of cationic additives. Decreased albumin concentration and increased cationic detergent concentration both increased the sensitivity of the assay due to kinetic effects, but addition of a low molecular weight cation had no effect. The reduction of NBT by glycated albumin was not increased in sensitivity by a protein matrix of low concentration. Due to the complexity and poorly understood nature of interaction between DMF and albumin in the reduction of NBT, the authors do not consider DMF to be a suitable primary calibrant for the fructosamine assay.

Interaction of Cationic Proteins and Polypeptides with Biocompatible Cationically-Anchored PEG Brushes

2011 ◽  
Vol 44 (20) ◽  
pp. 8161-8168 ◽  
Author(s):  
S. Gon ◽  
B. Fang ◽  
M. M. Santore
Keyword(s):  
Cationic Proteins
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