Effect of electric field switching on the electrophoretic mobility of single-stranded DNA molecules in polyacrylamide gels

1989 ◽  
Vol 10 (1) ◽  
pp. 65-67 ◽  
Author(s):  
Eric Lai ◽  
Noelle A. Davi ◽  
Leroy E. Hood
Keyword(s):  
Electric Field ◽  
Electrophoretic Mobility ◽  
Polyacrylamide Gels ◽  
Dna Molecules ◽  
Single Stranded Dna

Glycophorin expression in murine erythroleukaemia cells

1989 ◽  
Vol 92 (2) ◽  
pp. 163-171
Author(s):  
J.B. Ulmer ◽  
E.D. Dolci ◽  
G.E. Palade
Keyword(s):  
Cell Line ◽  
Electrophoretic Mobility ◽  
Normal Mouse ◽  
Fatty Acyl ◽  
Dimethyl Sulphoxide ◽  
Partial Characterization ◽  
Polyacrylamide Gels ◽  
Putative Precursor

We have identified mature and putative precursor forms of glycophorins expressed in a virus-transformed murine erythroleukaemia (MEL) cell line and compared them with their normal erythroblast counterparts. The following differences were found: (1) the two major MEL cell glycophorins (apparent Mr values 29–30 and 43(x10(3] have greater mobility on polyacrylamide gels than their normal gp-3 and gp-2 counterparts, due at least in part to differences in their oligosaccharide sidechains; (2) MEL cell gp-3 consists of two discrete proteins; and (3) there are more potential glycophorin precursors in MEL cells than in normal mouse erythroblasts. Four proteins, with apparent Mr values of 21, 23, 26 and 27(x10(3], have tentatively been identified as glycophorin precursors, based on the following findings: (1) they are immunologically related to the glycophorins; and (2) their synthesis was induced by dimethyl sulphoxide coincidentally with that of gp-3 and gp-2. They do not appear to be glycoproteins, as evidenced by their lack of incorporation of [3H]galactose, [3H]glucosamine or [3H]mannose. In contrast, gp-3 and gp-2 incorporated [3H]galactose and [3H]glucosamine but not [3H]mannose. Partial characterization of the glycan moieties of MEL cell glycophorins indicates that they consist mostly of tri- and tetrasaccharides, with no indication of any N-linked chains. Hence, the glycans of MEL cell glycophorins are mostly (if not all) O-linked. Furthermore, treatment with N-glycanase did not change their electrophoretic mobility on polyacrylamide gels. MEL cell glycophorins were also shown to be modified by phosphoryl and fatty acyl groups.

PCR-BASED SYNTHESIS OF REPETITIVE SINGLE-STRANDED DNA FOR APPLICATIONS TO NANOBIOTECHNOLOGY

2005 ◽  
Vol 04 (03) ◽  
pp. 287-294
Author(s):  
SIMA S. ZEIN ◽  
ALEXANDRE A. VETCHER ◽  
STEPHEN D. LEVENE
Keyword(s):  
Dna Sequences ◽  
Tandem Repeats ◽  
Repetitive Sequences ◽  
Practical Interest ◽  
Telomeric Sequence ◽  
Automated Synthesis ◽  
Dna Molecules ◽  
Specific Sequence ◽  
Single Stranded Dna ◽  
Pcr Method

Recent data show that assembly of repetitive-sequence, single-stranded DNA molecules (ssDNA) and carbon nanotubes (CNTs) depend on the specific sequence repeat. Therefore, it is of practical interest to assess various methods for generating single-stranded DNA molecules that contain repetitive sequences. Existing automated synthesis procedures for generating long (> 100 nt) ssDNA molecules generate ssDNA products of variable purity and yield. An alternative to automated synthesis is the polymerase chain reaction (PCR), which provides a powerful tool for the amplification of minute amounts of specific DNA sequences. Here we show that a modified asymmetric PCR method allows synthesis of long ssDNAs comprised of tandem repeats of the repetitive vertebrate telomeric sequence (TTAGGG)n, and is also applicable to arbitrary (repetitive or nonrepetitive) DNA. Long, repetitive deoxynucleotides produced by automated synthesis are surprisingly heterogeneous with respect to both length and sequence. Benefits of the method described here are that long, repetitive ssDNA sequences are generated with high sequence fidelity and yield.

Membrane-free electroextraction using an aqueous two-phase system

RSC Advances ◽  
2014 ◽  
Vol 4 (90) ◽  
pp. 49485-49490 ◽  
Author(s):  
C. D. M. Campos ◽  
J. K. Park ◽  
P. Neužil ◽  
J. A. F. da Silva ◽  
A. Manz
Keyword(s):  
Amino Acids ◽  
Electric Field ◽  
Electrophoretic Mobility ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
System P

We present a method of continuous electroextraction of amino acids using aqueous two phase system in a microchip. The separations occur due to differences in electrophoretic mobility and solvent affinity. The results suggest the possibility of high levels of purification by controlling the electric field across the liquid barrier.

scholarly journals Model Checking Temporal Logic Formulas Using Sticker Automata

2017 ◽  
Vol 2017 ◽  
pp. 1-33 ◽  
Author(s):  
Weijun Zhu ◽  
Changwei Feng ◽  
Huanmei Wu
Keyword(s):  
Model Checking ◽  
Temporal Logic ◽  
Complex Problem ◽  
Finite State Automaton ◽  
Dna Molecules ◽  
Single Stranded Dna ◽  
Finite State ◽  
Input Strings ◽  
Logic Model Checking ◽  
The Given

As an important complex problem, the temporal logic model checking problem is still far from being fully resolved under the circumstance of DNA computing, especially Computation Tree Logic (CTL), Interval Temporal Logic (ITL), and Projection Temporal Logic (PTL), because there is still a lack of approaches for DNA model checking. To address this challenge, a model checking method is proposed for checking the basic formulas in the above three temporal logic types with DNA molecules. First, one-type single-stranded DNA molecules are employed to encode the Finite State Automaton (FSA) model of the given basic formula so that a sticker automaton is obtained. On the other hand, other single-stranded DNA molecules are employed to encode the given system model so that the input strings of the sticker automaton are obtained. Next, a series of biochemical reactions are conducted between the above two types of single-stranded DNA molecules. It can then be decided whether the system satisfies the formula or not. As a result, we have developed a DNA-based approach for checking all the basic formulas of CTL, ITL, and PTL. The simulated results demonstrate the effectiveness of the new method.

scholarly journals DNA translocation to giant unilamellar vesicles during electroporation is independent of DNA size

Soft Matter ◽  
2019 ◽  
Vol 15 (45) ◽  
pp. 9187-9194
Author(s):  
Shaurya Sachdev ◽  
Aswin Muralidharan ◽  
Dipendra K. Choudhary ◽  
Dayinta L. Perrier ◽  
Lea Rems ◽  
...  
Keyword(s):  
Electrophoretic Mobility ◽  
Dna Delivery ◽  
Dna Molecules ◽  
Dna Translocation ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Dna Size

DNA delivery into GUVs during electroporation is governed by bulk electrophoretic mobility implying a mechanism in which DNA molecules enter in their coiled conformation, as opposed to stochastic threading, through electro-pores.

Deposition and Characterization of Extended Single-Stranded DNA Molecules on Surfaces

Nano Letters ◽  
2001 ◽  
Vol 1 (7) ◽  
pp. 345-348 ◽  
Author(s):  
Adam T. Woolley ◽  
Ryan T. Kelly
Keyword(s):  
Dna Molecules ◽  
Single Stranded Dna
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