Electrochemical Signal Enhancer Fabricated Using Lysine‐rich Peptide for Ultrasensitive Electrochemical DNA Biosensor Analysis

2019 ◽  
Vol 31 (4) ◽  
pp. 607-610
Author(s):  
Ying Yuan ◽  
Zhe Xun ◽  
Bin Zhang ◽  
Yifu Guan
Keyword(s):  
Dna Biosensor ◽  
Electrochemical Dna Biosensor ◽  
Electrochemical Signal ◽  
Biosensor Analysis

scholarly journals A DNA Electrochemical Sensor via Terminal Protection of Small-Molecule-Linked DNA for Highly Sensitive Protein Detection

Biosensors ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 451
Author(s):  
Ping Ouyang ◽  
Chenxin Fang ◽  
Jialun Han ◽  
Jingjing Zhang ◽  
Yuxing Yang ◽  
...  
Keyword(s):  
Small Molecule ◽  
Gold Electrode ◽  
Folate Receptor ◽  
Protein Detection ◽  
Disease Diagnosis ◽  
Dna Biosensor ◽  
Target Protein ◽  
Receptor Protein ◽  
Electrochemical Dna Biosensor ◽  
Electrochemical Signal

The qualitative and quantitative determination of marker protein is of great significance in the life sciences and in medicine. Here, we developed an electrochemical DNA biosensor for protein detection based on DNA self-assembly and the terminal protecting effects of small-molecule-linked DNA. This strategy is demonstrated using the small molecule biotin and its receptor protein streptavidin (SA). We immobilized DNA with a designed structure and sequence on the surface of the gold electrode, and we named it M1-Biotin DNA. M1-Biotin DNA selectively combines with SA to generate M1-Biotin-SA DNA and protects M1-Biotin DNA from digestion by EXO III; therefore, M1-Biotin DNA remains intact on the electrode surface. M1-Biotin-SA DNA was modified with methylene blue (MB); the MB reporter molecule is located near the surface of the gold electrode, which generates a substantial electrochemical signal during the detection of SA. Through this strategy, we can exploit the presence or absence of an electrochemical signal to provide qualitative target protein determination as well as the strength of the electrochemical signal to quantitatively analyze the target protein concentration. This strategy has been proven to be used for the quantitative analysis of the interaction between biotin and streptavidin (SA). Under optimal conditions, the detection limit of the proposed biosensor is as low as 18.8 pM, and the linear range is from 0.5 nM to 5 μM, showing high sensitivity. The detection ability of this DNA biosensor in complex serum samples has also been studied. At the same time, we detected the folate receptor (FR) to confirm that this strategy can be used to detect other proteins. Therefore, this electrochemical DNA biosensor provides a sensitive, low-cost, and fast target protein detection platform, which may provide a reliable and powerful tool for early disease diagnosis.

Electrochemical DNA biosensor for the detection of specific gene related to Microcystis species

2001 ◽  
Vol 3 (5) ◽  
pp. 224-228 ◽  
Author(s):  
Fei Yan ◽  
Arzum Erdem ◽  
Burcu Meric ◽  
Kagan Kerman ◽  
Mehmet Ozsoz ◽  
...  
Keyword(s):  
Dna Biosensor ◽  
Specific Gene ◽  
Electrochemical Dna Biosensor

scholarly journals Identification of Chinese Herbs Using a Sequencing-Free Nanostructured Electrochemical DNA Biosensor

Sensors ◽  
2015 ◽  
Vol 15 (12) ◽  
pp. 29882-29892 ◽  
Author(s):  
Yan Lei ◽  
Fan Yang ◽  
Lina Tang ◽  
Keli Chen ◽  
Guo-Jun Zhang
Keyword(s):  
Dna Biosensor ◽  
Chinese Herbs ◽  
Electrochemical Dna Biosensor

A Sensitive Nanoporous Gold-Based Electrochemical DNA Biosensor forEscherichia coliDetection

2011 ◽  
Vol 44 (16) ◽  
pp. 2559-2570 ◽  
Author(s):  
Kang Li ◽  
Jingjing Huang ◽  
Guoyue Shi ◽  
Wen Zhang ◽  
Litong Jin
Keyword(s):  
Dna Biosensor ◽  
Nanoporous Gold ◽  
Electrochemical Dna Biosensor

Electrochemical DNA biosensor for the study of ciprofloxacin–DNA interaction

2006 ◽  
Vol 354 (1) ◽  
pp. 28-34 ◽  
Author(s):  
Haq Nawaz ◽  
Sakandar Rauf ◽  
Kalsoom Akhtar ◽  
Ahmad M. Khalid
Keyword(s):  
Dna Interaction ◽  
Dna Biosensor ◽  
Electrochemical Dna Biosensor

Rapid and label-free electrochemical DNA biosensor for detecting hepatitis A virus

2018 ◽  
Vol 100 ◽  
pp. 89-95 ◽  
Author(s):  
Marisa Manzano ◽  
Sara Viezzi ◽  
Sandra Mazerat ◽  
Robert S. Marks ◽  
Jasmina Vidic
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Dna Biosensor ◽  
Label Free ◽  
Electrochemical Dna Biosensor
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