Ultrasensitive Genosensor Based on Minor Grove Binding (MGB) Probe forIL28BSingle Nucleotide Polymorphism (SNP) Detection Using SYBR Green as Electrochemical Indicator

2018 ◽  
Vol 30 (12) ◽  
pp. 2847-2852
Author(s):  
Luydson R. S. Vasconcelos ◽  
Patricia Moura ◽  
Rosa F. Dutra
Keyword(s):  
Sybr Green ◽  
Nucleotide Polymorphism ◽  
Snp Detection ◽  
Mgb Probe

scholarly journals Donor Chimerism Study by Single Nucleotide Polymorphism using SYBR green based Real Time PCR

2021 ◽  
Vol 37 (7) ◽  
Author(s):  
Ayesha Nayyar ◽  
Suhaib Ahmed
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Real Time ◽  
Real Time Pcr ◽  
Sybr Green ◽  
Haematopoietic Stem Cell ◽  
Nucleotide Polymorphism ◽  
Green Method ◽  
Single Nucleotide ◽  
Donor Chimerism ◽  
Haematological Disorders

Objective: To optimize and evaluate a real time PCR of Single Nucleotide Polymorphism by SYBR Green method for detection of donor chimerism after haematopoietic stem cell transplantation. Methods: This descriptive study was conducted at Genetic Resource Centre (GRC) Lab Rawalpindi from Oct 2017 - Dec 2019. A total of twenty patients of post haematopoietic stem cell transplant with various haematological disorders were studied to see the status of donor chimerism by using SNP real time PCR using SYBR Green method and short tandem repeat PCR. These patients had undergone allogeneic HSCT from HLA-matched sibling donors at Pakistan Institute of Medical Science and Armed Forces Bone Marrow Transplant Centre. Results: Real time PCR using SYBR Green was able to detect significant amount of chimerism in all 20 patients having undergone HSCT. Regarding precision of the real time PCR assay the mean value of donor chimerism was 94.1% (SD 3.96) and by STR PCR it was 95.1% (SD 1.41). The assay was found to be sensitive with a detection limit of <1%. Conclusion: Our results demonstrate that SNP analysis by SYBR Green real time PCR may be used for the evaluation of chimerism status in patients having undergone HSCT with a sensitivity of <1%. Hence donor chimerism by this sensitive method can be used in monitoring of chimerism in post-transplant patients with various haematological disorders. doi: https://doi.org/10.12669/pjms.37.7.4203 How to cite this:Nayyar A, Ahmed S. Donor Chimerism Study by Single Nucleotide Polymorphism using SYBR green based Real Time PCR. Pak J Med Sci. 2021;37(7):---------. doi: https://doi.org/10.12669/pjms.37.7.4203 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

A bead-based single nucleotide polymorphism (SNP) detection using melting temperature on a microchip

2014 ◽  
Vol 17 (3) ◽  
pp. 477-488 ◽  
Author(s):  
Pei-Chun Kao ◽  
Shih-Torng Ding ◽  
En-Chung Lin ◽  
Kan-Chien Li ◽  
Lon Wang ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Melting Temperature ◽  
Nucleotide Polymorphism ◽  
Snp Detection ◽  
Single Nucleotide

scholarly journals Pre-earthquake non-epidemic Vibrio cholerae in Haiti

2014 ◽  
Vol 8 (01) ◽  
pp. 120-122 ◽  
Author(s):  
Jie Liu ◽  
Christopher Winstead-Derlega ◽  
Eric Houpt ◽  
Rebecca Heidkamp ◽  
Jean Pape ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Sybr Green ◽  
Molecular Assays ◽  
Nutrition Study ◽  
Stool Samples ◽  
Pediatric Nutrition ◽  
Mgb Probe ◽  
Taqman Mgb Probe

Introduction: To our knowledge, there was no record of Vibrio cholerae in Haiti until the 2010 post earthquake outbreak. Methodology: This study describes the analysis of 301 stool samples from 117 infants in Port-au-Prince, Haiti, who participated in a pediatric nutrition study between July 2008 and October 2009. Results: Nine samples were identified positive with both SYBR Green and Taqman-MGB probe based molecular assays targeting V. cholerae hlyA and toxR, respectively (Ct = 33 – 40), but none were O1 or O139. Conclusions: Our results from multiple molecular assays demonstrate the presence of non-O1/O139 V. cholerae DNA in stools collected from nine asymptomatic Haitian infants two years prior to the 2010 earthquake.

A method for SNP detection using MoS2@AuNPs and SYBR Green I in combination with enzyme digestion

2019 ◽  
Vol 43 (47) ◽  
pp. 18571-18574
Author(s):  
Lirong Yan ◽  
Zebin Deng ◽  
Haixia Shi ◽  
Bing Xie ◽  
Li Gao
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Gene Sequence ◽  
Enzyme Digestion ◽  
Sybr Green ◽  
Sybr Green I ◽  
Human Diseases ◽  
Nucleotide Polymorphisms ◽  
Snp Detection ◽  
Single Nucleotide

Single-nucleotide polymorphisms (SNPs) in a gene sequence are markers for a variety of human diseases.

High-throughput ultra-sensitive discrimination of single nucleotide polymorphism via click chemical ligation

The Analyst ◽  
2020 ◽  
Vol 145 (1) ◽  
pp. 172-176
Author(s):  
Qian-Yu Zhou ◽  
Xin-Ying Zhong ◽  
Ling-Li Zhao ◽  
Li-Juan Wang ◽  
Ying-Lin Zhou ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
High Throughput ◽  
Nucleotide Polymorphism ◽  
Snp Detection ◽  
Single Nucleotide ◽  
Chemical Ligation ◽  
Ligation Reaction

CuAAC-based ligation-assisted assays: the CuAAC chemical ligation reaction for SNP detection.

Sign in / Sign up

Export Citation Format

Share Document