A Novel Solid-state Electrochemiluminescent Enantioselective Sensor for Ascorbic Acid and Isoascorbic Acid

2016 ◽  
Vol 29 (2) ◽  
pp. 466-471 ◽  
Author(s):  
Shu Zhu ◽  
Xia Lin ◽  
Qinghong Wang ◽  
Qiao Xia ◽  
Peiyao Ran ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Solid State ◽  
Isoascorbic Acid ◽  
Enantioselective Sensor

Fatty acid monoesters of 1-ascorbic acid and d-isoascorbic acid

1943 ◽  
Vol 20 (11) ◽  
pp. 224-226 ◽  
Author(s):  
Daniel Swern ◽  
A. J. Stirton ◽  
J. Turer ◽  
P. A. Wells
Keyword(s):  
Ascorbic Acid ◽  
Fatty Acid ◽  
Isoascorbic Acid

Requirement for Na(+)-dependent ascorbic acid transport in osteoblast function

1995 ◽  
Vol 268 (6) ◽  
pp. C1430-C1439 ◽  
Author(s):  
R. T. Franceschi ◽  
J. X. Wilson ◽  
S. J. Dixon
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Disulfonic Acid ◽  
Acid Transport ◽  
Transport Activity ◽  
Intracellular Accumulation ◽  
Extracellular Medium ◽  
Isoascorbic Acid ◽  
Transport Inhibitors ◽  
Ascorbic Acid Transport

Ascorbic acid is necessary for expression of the osteoblast phenotype. We examined whether Na(+)-dependent transport is required for MC3T3-E1 preosteoblast cells to respond to vitamin C and investigated the role of membrane transport in the intracellular accumulation and function of ascorbate. MC3T3-E1 cells were found to possess a saturable, stereoselective, Na(+)-dependent ascorbic acid transport activity that is sensitive to the transport inhibitors sulfinpyrazone, 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid, and phloretin. Transport activity showed no competition with glucose or 2-deoxyglucose and was not inhibited by cytochalasin B, indicating that it is distinct from known hexose transporters. On addition of 100 microM ascorbic acid to the extracellular medium, intracellular concentrations of 10 mM were reached within 5-10 h and remained constant for up to 24 h. A good correlation was observed between intracellular ascorbic acid concentration and rate of hydroxyproline synthesis. Although ascorbic acid was transported preferentially compared with D-isoascorbic acid, both isomers had equivalent activity in stimulating hydroxyproline formation once they entered cells. Marked stereoselectivity for extracellular L-ascorbic acid relative to D-isoascorbic acid was also seen when alkaline phosphatase and total hydroxyproline were measured after 6 days in culture. Moreover, ascorbic acid transport inhibitors that prevented intracellular accumulation of vitamin blocked the synthesis of hydroxyproline. Thus Na(+)-dependent ascorbic acid transport is required for MC3T3-E1 cells to achieve the millimolar intracellular vitamin C concentrations necessary for maximal prolyl hydroxylase activity and expression of the osteoblast phenotype.

Weak measurement-based sensor for the rapid identification of L(+)-ascorbic acid and D(-)-isoascorbic acid

2019 ◽  
Vol 58 (31) ◽  
pp. 8583
Author(s):  
Suyi Zhong ◽  
Tian Guan ◽  
Yang Xu ◽  
Chongqi Zhou ◽  
Lixuan Shi ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Rapid Identification ◽  
Weak Measurement ◽  
Isoascorbic Acid

Determination of ascorbic acid, dehydroascorbic acid, and isoascorbic acid in blood

1986 ◽  
Vol 325 (5) ◽  
pp. 473-475 ◽  
Author(s):  
Jörg Schreiber ◽  
Wolfgang Lohmann ◽  
Dietrich Unverzagt ◽  
Albert Otten
Keyword(s):  
Ascorbic Acid ◽  
Dehydroascorbic Acid ◽  
Isoascorbic Acid

ChemInform Abstract: Studies on Dehydro-L-ascorbic Acid and Dehydro-D-isoascorbic Acid Hydrazones.

ChemInform ◽  
2010 ◽  
Vol 30 (36) ◽  
pp. no-no
Author(s):  
Mohamed A. El-Sekily ◽  
Mohamed E. Elba ◽  
Farid S. Fouad
Keyword(s):  
Ascorbic Acid ◽  
Isoascorbic Acid

Transfer of D-isoascorbic acid and L-ascorbic acid into guinea pig eyes

1969 ◽  
Vol 26 (1) ◽  
pp. 164-170 ◽  
Author(s):  
Erik Linnér ◽  
Karin Nordström
Keyword(s):  
Ascorbic Acid ◽  
Guinea Pig ◽  
Isoascorbic Acid
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